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• W2124883150 abstract "Transforming growth factor (TGF) β1 facilitates FSH-induced differentiation of rat ovarian granulosa cells. The signaling crosstalk between follicle stimulating hormone (FSH) and TGFβ receptors remains unclear. This study was to investigate the interplay of cAMP/protein kinase A (PKA) and phosphatidylinositol-3-kinase (PI3K) signaling including mammalian target of rapamycin (mTOR)C1 dependence in FSH- and TGFβ1-stimulated steroidogenesis in rat granulosa cells. To achieve this aim, inhibitors of PKA (PKAI), PI3K (wortmannin), and mTORC1 (rapamycin) were employed. PKAI and wortmannin suppressions of the FSH-increased progesterone production were partly attributed to decreased level of 3β-HSD, and their suppression of the FSH plus TGFβ1 effect was attributed to the reduction of all the three key players, steroidogenic acute regulatory (StAR) protein, P450scc, and 3β-HSD. Further, FSH activated the PI3K pathway including increased integrin-linked kinase (ILK) activity and phosphorylation of Akt(S473), mTOR(S2481), S6K(T389), and transcription factors particularly FoxO1(S256) and FoxO3a(S253), which were reduced by wortmannin treatment but not by PKAI. Interestingly, PKAI suppression of FSH-induced phosphorylation of cAMP regulatory element-binding protein (CREB(S133)) disappeared in the presence of wortmannin, suggesting that wortmannin may affect intracellular compartmentalization of signaling molecule(s). In addition, TGFβ1 had no effect on FSH-activated CREB and PI3K signaling mediators. We further found that rapamycin reduced the TGFβ1-enhancing effect of FSH-stimulated steroidogenesis, yet it exhibited no effect on FSH action. Surprisingly, rapamycin displayed a suppressive effect at concentrations that had no effect on mTORC1 activity. Together, this study demonstrates a delicate interplay between cAMP/PKA and PI3K signaling in FSH and TGFβ1 regulation of steroidogenesis in rat granulosa cells. Furthermore, we demonstrate for the first time that TGFβ1 acts in a rapamycin-hypersensitive and mTORC1-independent manner in augmenting FSH-stimulated steroidogenesis in rat granulosa cells." @default.
• W2124883150 created "2016-06-24" @default.
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• W2124883150 date "2007-02-01" @default.
• W2124883150 modified "2023-09-30" @default.
• W2124883150 title "Interplay of PI3K and cAMP/PKA signaling, and rapamycin-hypersensitivity in TGFβ1 enhancement of FSH-stimulated steroidogenesis in rat ovarian granulosa cells" @default.
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• W2124883150 cites W1580323249 @default.
• W2124883150 cites W1610204701 @default.
• W2124883150 cites W1964737314 @default.
• W2124883150 cites W1965980703 @default.
• W2124883150 cites W1969054713 @default.
• W2124883150 cites W1970907948 @default.
• W2124883150 cites W1974955986 @default.
• W2124883150 cites W1976320711 @default.
• W2124883150 cites W1983628734 @default.
• W2124883150 cites W1984860957 @default.
• W2124883150 cites W1989608005 @default.
• W2124883150 cites W1991088949 @default.
• W2124883150 cites W1992506198 @default.
• W2124883150 cites W2000099153 @default.
• W2124883150 cites W2002572737 @default.
• W2124883150 cites W2007530728 @default.
• W2124883150 cites W2008827433 @default.
• W2124883150 cites W2010580307 @default.
• W2124883150 cites W2010637356 @default.
• W2124883150 cites W2010980536 @default.
• W2124883150 cites W2014840069 @default.
• W2124883150 cites W2016324935 @default.
• W2124883150 cites W2017882961 @default.
• W2124883150 cites W2019788712 @default.
• W2124883150 cites W2021283573 @default.
• W2124883150 cites W2024774077 @default.
• W2124883150 cites W2034691869 @default.
• W2124883150 cites W2037217477 @default.
• W2124883150 cites W2037341437 @default.
• W2124883150 cites W2038988941 @default.
• W2124883150 cites W2041926656 @default.
• W2124883150 cites W2043447724 @default.
• W2124883150 cites W2051338141 @default.
• W2124883150 cites W2052583546 @default.
• W2124883150 cites W2053428839 @default.
• W2124883150 cites W2056891806 @default.
• W2124883150 cites W2063840368 @default.
• W2124883150 cites W2064647513 @default.
• W2124883150 cites W2064738912 @default.
• W2124883150 cites W2076103389 @default.
• W2124883150 cites W2088359631 @default.
• W2124883150 cites W2090558875 @default.
• W2124883150 cites W2102112763 @default.
• W2124883150 cites W2105963133 @default.
• W2124883150 cites W2111031460 @default.
• W2124883150 cites W2113002082 @default.
• W2124883150 cites W2115593116 @default.
• W2124883150 cites W2118120822 @default.
• W2124883150 cites W2124080367 @default.
• W2124883150 cites W2134138119 @default.
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• W2124883150 doi "https://doi.org/10.1677/joe-06-0076" @default.
• W2124883150 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17283241" @default.
• W2124883150 hasPublicationYear "2007" @default.
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