Matches in SemOpenAlex for { <https://semopenalex.org/work/W2124902009> ?p ?o ?g. }
- W2124902009 endingPage "5138" @default.
- W2124902009 startingPage "5130" @default.
- W2124902009 abstract "In the present study, we have used single chicken blastoderms of defined early developmental stages, beginning with the prestreak stage, stage 1 (V. Hamburger and H. L. Hamilton, J. Morphol. 88:49-92, 1951), to analyze the onset of cardiac myogenesis by monitoring the appearance of selected cardiac muscle tissue-specific gene transcripts and the functional expression of the myocyte enhancer factor 2 (MEF-2) proteins. Using gene-specific oligonucleotide primers in reverse transcriptase PCR assay, we have demonstrated that the cardiac myosin light-chain 2 (MLC2) and alpha-actin gene transcripts appear as early as stage 5, i.e., immediately after the cardiogenic fate assignment at stage 4. Consistent with this observation is the developmental expression pattern of DNA-binding activity of BBF-1, a cardiac muscle-specific member of the MEF-2 protein family, which also begins at stage 5 prior to MEF-2. Differential expression of DNA-binding complexes is also observed with another AT-rich DNA sequence (CArG box) as probe, but the binding pattern with the ubiquitous TATA-binding proteins remains unchanged during the same developmental period. Thus, the cardiogenic commitment and differentiation of the precardiac mesoderm, as exemplified by the appearance of cardiac MEF-2, MLC2, and alpha-actin gene products, occur earlier than previously thought and appear to be closely linked. The onset of skeletal myogenic program follows that of the cardiogenic program with the appearance of skeletal MLC2 at stage 8. We also observed that mRNA for the MEF-2 family of proteins appears as early as stage 2 and that for CMD-1, the chicken counterpart of MyoD, appears at stage 5. The temporal separation of activation of cardiac and skeletal MLC2 genes, which appears immediately after the respective fate assignments, and those of cardiac MEF-2 and CMD-1, which occur before, are consistent with the established appearance of the myogenic programs and with the acquisition pattern of the two tissue-specific morphological characteristics in the early embryo. The preferential appearance of BBF-1 activity in precardiac moesderm, relative to that of MEF-2, indicates that these two protein factors are distinct members of the MEF-2 family and provides a compelling argument in support of the potential role of BBF-1 as a regulator of the cardiogenic cell lineage determination, while cardiac MEF-2 might be involved in maintenance of the cardiac differentiative state." @default.
- W2124902009 created "2016-06-24" @default.
- W2124902009 creator A5001195126 @default.
- W2124902009 creator A5004967550 @default.
- W2124902009 creator A5023408129 @default.
- W2124902009 creator A5025787914 @default.
- W2124902009 creator A5037579723 @default.
- W2124902009 creator A5061797233 @default.
- W2124902009 creator A5073778401 @default.
- W2124902009 date "1994-08-01" @default.
- W2124902009 modified "2023-09-25" @default.
- W2124902009 title "Differential expression of the myocyte enhancer factor 2 family of transcription factors in development: the cardiac factor BBF-1 is an early marker for cardiogenesis" @default.
- W2124902009 cites W1482998738 @default.
- W2124902009 cites W1525121830 @default.
- W2124902009 cites W157094490 @default.
- W2124902009 cites W1595752904 @default.
- W2124902009 cites W1617868157 @default.
- W2124902009 cites W1810995470 @default.
- W2124902009 cites W1867816903 @default.
- W2124902009 cites W1877288892 @default.
- W2124902009 cites W1878061663 @default.
- W2124902009 cites W1964733862 @default.
- W2124902009 cites W1972711239 @default.
- W2124902009 cites W1978995496 @default.
- W2124902009 cites W1981344985 @default.
- W2124902009 cites W1982726291 @default.
- W2124902009 cites W1982744028 @default.
- W2124902009 cites W1983438549 @default.
- W2124902009 cites W1984143972 @default.
- W2124902009 cites W1984536688 @default.
- W2124902009 cites W1992879938 @default.
- W2124902009 cites W1993243592 @default.
- W2124902009 cites W2002974644 @default.
- W2124902009 cites W2003708679 @default.
- W2124902009 cites W2017765584 @default.
- W2124902009 cites W2020522024 @default.
- W2124902009 cites W2029172751 @default.
- W2124902009 cites W2037427273 @default.
- W2124902009 cites W2042752855 @default.
- W2124902009 cites W2047926713 @default.
- W2124902009 cites W2057249507 @default.
- W2124902009 cites W2070293940 @default.
- W2124902009 cites W2070388090 @default.
- W2124902009 cites W2074404562 @default.
- W2124902009 cites W2087363523 @default.
- W2124902009 cites W2088769654 @default.
- W2124902009 cites W2088956217 @default.
- W2124902009 cites W2106438297 @default.
- W2124902009 cites W2107844605 @default.
- W2124902009 cites W2113420985 @default.
- W2124902009 cites W2115994363 @default.
- W2124902009 cites W2118554742 @default.
- W2124902009 cites W2122967431 @default.
- W2124902009 cites W2140034535 @default.
- W2124902009 cites W2145844911 @default.
- W2124902009 cites W2159814562 @default.
- W2124902009 cites W2164340322 @default.
- W2124902009 cites W2177035195 @default.
- W2124902009 cites W2297659775 @default.
- W2124902009 cites W2299104974 @default.
- W2124902009 cites W2385672755 @default.
- W2124902009 cites W2474443891 @default.
- W2124902009 cites W4248096216 @default.
- W2124902009 doi "https://doi.org/10.1128/mcb.14.8.5130-5138.1994" @default.
- W2124902009 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/359032" @default.
- W2124902009 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8035795" @default.
- W2124902009 hasPublicationYear "1994" @default.
- W2124902009 type Work @default.
- W2124902009 sameAs 2124902009 @default.
- W2124902009 citedByCount "8" @default.
- W2124902009 crossrefType "journal-article" @default.
- W2124902009 hasAuthorship W2124902009A5001195126 @default.
- W2124902009 hasAuthorship W2124902009A5004967550 @default.
- W2124902009 hasAuthorship W2124902009A5023408129 @default.
- W2124902009 hasAuthorship W2124902009A5025787914 @default.
- W2124902009 hasAuthorship W2124902009A5037579723 @default.
- W2124902009 hasAuthorship W2124902009A5061797233 @default.
- W2124902009 hasAuthorship W2124902009A5073778401 @default.
- W2124902009 hasConcept C104317684 @default.
- W2124902009 hasConcept C111936080 @default.
- W2124902009 hasConcept C145103041 @default.
- W2124902009 hasConcept C150194340 @default.
- W2124902009 hasConcept C153911025 @default.
- W2124902009 hasConcept C207200792 @default.
- W2124902009 hasConcept C2776657240 @default.
- W2124902009 hasConcept C2780915607 @default.
- W2124902009 hasConcept C3763915 @default.
- W2124902009 hasConcept C51183755 @default.
- W2124902009 hasConcept C54355233 @default.
- W2124902009 hasConcept C6997183 @default.
- W2124902009 hasConcept C86339819 @default.
- W2124902009 hasConcept C86803240 @default.
- W2124902009 hasConcept C95444343 @default.
- W2124902009 hasConceptScore W2124902009C104317684 @default.
- W2124902009 hasConceptScore W2124902009C111936080 @default.
- W2124902009 hasConceptScore W2124902009C145103041 @default.
- W2124902009 hasConceptScore W2124902009C150194340 @default.
- W2124902009 hasConceptScore W2124902009C153911025 @default.