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- W2124930417 abstract "Abstract Recently, a number of two LIM-domain containing proteins (LIMs) have been reported to trigger the formation of actin bundles, a major higher-order cytoskeletal assembly. Here, we analyzed the six Arabidopsis thaliana LIM proteins. Promoter-β-glucuronidase reporter studies revealed that WLIM1, WLIM2a, and WLIM2b are widely expressed, whereas PLIM2a, PLIM2b, and PLIM2c are predominantly expressed in pollen. LIM-green fluorescent protein (GFP) fusions all decorated the actin cytoskeleton and increased actin bundle thickness in transgenic plants and in vitro, although with different affinities and efficiencies. Remarkably, the activities of WLIMs were calcium and pH independent, whereas those of PLIMs were inhibited by high pH and, in the case of PLIM2c, by high [Ca2+]. Domain analysis showed that the C-terminal domain is key for the responsiveness of PLIM2c to pH and calcium. Regulation of LIM by pH was further analyzed in vivo by tracking GFP-WLIM1 and GFP-PLIM2c during intracellular pH modifications. Cytoplasmic alkalinization specifically promoted release of GFP-PLIM2c but not GFP-WLIM1, from filamentous actin. Consistent with these data, GFP-PLIM2c decorated long actin bundles in the pollen tube shank, a region of relatively low pH. Together, our data support a prominent role of Arabidopsis LIM proteins in the regulation of actin cytoskeleton organization and dynamics in sporophytic tissues and pollen." @default.
- W2124930417 created "2016-06-24" @default.
- W2124930417 creator A5000853855 @default.
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- W2124930417 date "2010-09-01" @default.
- W2124930417 modified "2023-10-03" @default.
- W2124930417 title "<i>Arabidopsis</i>LIM Proteins: A Family of Actin Bundlers with Distinct Expression Patterns and Modes of Regulation " @default.
- W2124930417 cites W1508867022 @default.
- W2124930417 cites W1538490065 @default.
- W2124930417 cites W1542867093 @default.
- W2124930417 cites W1923070859 @default.
- W2124930417 cites W1969409986 @default.
- W2124930417 cites W1971213954 @default.
- W2124930417 cites W1977551299 @default.
- W2124930417 cites W1977669212 @default.
- W2124930417 cites W1989108940 @default.
- W2124930417 cites W1989675697 @default.
- W2124930417 cites W1992633813 @default.
- W2124930417 cites W1996060069 @default.
- W2124930417 cites W2000318548 @default.
- W2124930417 cites W2005182674 @default.
- W2124930417 cites W2016180548 @default.
- W2124930417 cites W2020716393 @default.
- W2124930417 cites W2021812865 @default.
- W2124930417 cites W2030482324 @default.
- W2124930417 cites W2037424747 @default.
- W2124930417 cites W2038942260 @default.
- W2124930417 cites W2053388193 @default.
- W2124930417 cites W2060007367 @default.
- W2124930417 cites W2063193443 @default.
- W2124930417 cites W2063373328 @default.
- W2124930417 cites W2066276302 @default.
- W2124930417 cites W2068412323 @default.
- W2124930417 cites W2068671266 @default.
- W2124930417 cites W2071044193 @default.
- W2124930417 cites W2071330534 @default.
- W2124930417 cites W2071390801 @default.
- W2124930417 cites W2071775402 @default.
- W2124930417 cites W2072951880 @default.
- W2124930417 cites W2081950746 @default.
- W2124930417 cites W2084796550 @default.
- W2124930417 cites W2090287625 @default.
- W2124930417 cites W2091365889 @default.
- W2124930417 cites W2097098401 @default.
- W2124930417 cites W2105981507 @default.
- W2124930417 cites W2109304139 @default.
- W2124930417 cites W2110424522 @default.
- W2124930417 cites W2119463379 @default.
- W2124930417 cites W2121133562 @default.
- W2124930417 cites W2122113175 @default.
- W2124930417 cites W2122195365 @default.
- W2124930417 cites W2122249824 @default.
- W2124930417 cites W2122814701 @default.
- W2124930417 cites W2123058210 @default.
- W2124930417 cites W2129325793 @default.
- W2124930417 cites W2130420613 @default.
- W2124930417 cites W2133511144 @default.
- W2124930417 cites W2135188449 @default.
- W2124930417 cites W2136790795 @default.
- W2124930417 cites W2137688663 @default.
- W2124930417 cites W2138895739 @default.
- W2124930417 cites W2141143796 @default.
- W2124930417 cites W2142027485 @default.
- W2124930417 cites W2144591087 @default.
- W2124930417 cites W2145130198 @default.
- W2124930417 cites W2147170044 @default.
- W2124930417 cites W2150385698 @default.
- W2124930417 cites W2150729808 @default.
- W2124930417 cites W2151010232 @default.
- W2124930417 cites W2151131789 @default.
- W2124930417 cites W2155686789 @default.
- W2124930417 cites W2156919989 @default.
- W2124930417 cites W2157990737 @default.
- W2124930417 cites W2159586783 @default.
- W2124930417 cites W2159778631 @default.
- W2124930417 cites W2161742799 @default.
- W2124930417 cites W2162712339 @default.
- W2124930417 cites W2163448850 @default.
- W2124930417 cites W2163799721 @default.
- W2124930417 cites W2164434529 @default.
- W2124930417 cites W2165803331 @default.
- W2124930417 cites W2166460930 @default.
- W2124930417 cites W2169581687 @default.
- W2124930417 cites W2181487555 @default.
- W2124930417 cites W2332170386 @default.
- W2124930417 cites W4206803503 @default.
- W2124930417 cites W4238544418 @default.
- W2124930417 doi "https://doi.org/10.1105/tpc.110.075960" @default.
- W2124930417 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2965535" @default.
- W2124930417 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20817848" @default.
- W2124930417 hasPublicationYear "2010" @default.
- W2124930417 type Work @default.