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• W2125014734 abstract "Starfish oocyte maturation was blocked by the addition of 100 microM MG115, a potent proteasome inhibitor, whereas no inhibition was observed by membrane permeable cysteine protease inhibitor, E-64-d. The inhibition by MG115 was diminished by adding at a time corresponding to the half time required for germinal vesicle breakdown. Potent inhibition of germinal vesicle breakdown was also observed by microinjection of anti-proteasome-a-subunit antibodies. The antibody-injected oocytes failed to activate pre-maturation promoting factor (pre-MPF), since the dephosphorylation of phospho-Tyr15 in cdc2 kinase was not observed even in the presence of 1-methyadenine, a maturation-inducing hormone. These results indicate that the proteasome triggers the activation of pre-MPF via the dephosphorylation of cdc2 kinase in the signal transduction pathway in response to the hormonal stimulus during starfish oocyte maturation." @default.
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• W2125014734 date "1997-07-01" @default.
• W2125014734 modified "2023-09-25" @default.
• W2125014734 title "The Proteasome Is an Essential Mediator of the Activation of Pre-MPF during Starfish Oocyte Maturation" @default.
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• W2125014734 doi "https://doi.org/10.1006/bbrc.1997.6900" @default.
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