Matches in SemOpenAlex for { <https://semopenalex.org/work/W2125055963> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2125055963 endingPage "472" @default.
- W2125055963 startingPage "466" @default.
- W2125055963 abstract "식품 중 P. mirifica 원료 함유여부에 대한 판별법 마련을 위하여 식물의 종 동정에 일반적으로 사용되는 ribulose bisphosphate carboxylase (rbcL), RNA polymerase C (rpoC1), intergenic spacer (psbA-trnH) 및 second internal transcribed spacer (ITS2) 유전자부위를 선정하였다. 선정된 유전자부위를 증폭하기 위하여 일반 프라이머를 이용하였으며 각각 719 bp, 520 bp, 348 bp 및 507 bp의 PCR 산물을 확인하였다. 그리고 염기서열을 결정하고 유전자은행에 등록되어있는 염기서열과 유사성에 대한 분석한 결과 rbcL, rpoC1 및 psbA-trnH 부위는 상동성이 매우 높아 프라이머를 설계하기는 어려웠다. 그러나 ITS2의 경우 염기서열의 차이점이 있어 4종류의 프라이머를 설계하였다. 설계된 프라이머를 이용하여 P. mirifica, P. lobata, B. superba에 대한 PCR을 실시한 결과 SFI12-miri-6F/SFI12-miri-7R 및 SFI12-miri-6F/SFI12-miri-8R의 경우 P. lobata 와 B. superba에서 비 특이적 밴드가 없으며 P. mirifica에서 예상크기인 137 bp 및 216 bp를 확인할 수 있었다. 따라서 본 연구에서 개발된 P. mirifica을 판별할 수 있는 종 특이 프라이머는 식품가능원료 및 가공식품에 대한 적용할 수 있어 인터넷 쇼핑몰 등 시중에 불법적으로 유통되는 제품에 대한 안전관리에 활용도가 매우 클 것으로 기대된다. In this study, ribulose bisphosphate carboxylase (rbcL), RNApolymeraseC (rpoC1), intergenic spacer (psbA-trnH), and second internal transcribed spacer (ITS2) as identification markers for discrimination of P. mirifica in foods were selected. To be primer design, we obtained 719 bp, 520 bp, 348 bp, and 507 bp amplicon using universal primers from selected regions of P. mirifica. The regions of rbcL, rpoC1, and psbA-trnH were not proper for design primers because of high homology about P. mirifica, P. lobata, and B. superba. But, we had designed 4 pairs of oligonucleotide primers from ITS2 gene. Predicted amplicon from P. mirifica were obtained 137 bp and 216 bp using finally designed primers SFI12-miri-6F/SFI12-miri-7R and SFI12-miri-6F/SFI12-miri-8R, respectively. The species-specific primers distinguished P. mirifica from related species were able to apply food materials and processed foods. The developed PCR method would be applicable to food safety management for illegally distributed products in markets and internet shopping malls." @default.
- W2125055963 created "2016-06-24" @default.
- W2125055963 creator A5021479204 @default.
- W2125055963 creator A5037204409 @default.
- W2125055963 creator A5053222940 @default.
- W2125055963 creator A5057066324 @default.
- W2125055963 creator A5061666247 @default.
- W2125055963 creator A5068318933 @default.
- W2125055963 creator A5083556911 @default.
- W2125055963 creator A5089093274 @default.
- W2125055963 creator A5089229546 @default.
- W2125055963 date "2012-12-31" @default.
- W2125055963 modified "2023-10-16" @default.
- W2125055963 title "Detection Method for Identification of Pueraria mirifica (Thai kudzu) in Processed Foods" @default.
- W2125055963 cites W1037878827 @default.
- W2125055963 cites W1525730410 @default.
- W2125055963 cites W1527548437 @default.
- W2125055963 cites W1981662554 @default.
- W2125055963 cites W1997789531 @default.
- W2125055963 cites W1999520178 @default.
- W2125055963 cites W2012346381 @default.
- W2125055963 cites W2023356179 @default.
- W2125055963 cites W2030039555 @default.
- W2125055963 cites W2045500657 @default.
- W2125055963 cites W2059017897 @default.
- W2125055963 cites W2068626323 @default.
- W2125055963 cites W2069634931 @default.
- W2125055963 cites W2085525886 @default.
- W2125055963 cites W2099901798 @default.
- W2125055963 cites W2112762122 @default.
- W2125055963 cites W2129547418 @default.
- W2125055963 cites W2146320430 @default.
- W2125055963 cites W2171114940 @default.
- W2125055963 cites W2172820226 @default.
- W2125055963 cites W2329730978 @default.
- W2125055963 cites W2333855215 @default.
- W2125055963 cites W857519267 @default.
- W2125055963 cites W160379992 @default.
- W2125055963 doi "https://doi.org/10.13103/jfhs.2012.27.4.466" @default.
- W2125055963 hasPublicationYear "2012" @default.
- W2125055963 type Work @default.
- W2125055963 sameAs 2125055963 @default.
- W2125055963 citedByCount "3" @default.
- W2125055963 countsByYear W21250559632013 @default.
- W2125055963 countsByYear W21250559632015 @default.
- W2125055963 countsByYear W21250559632017 @default.
- W2125055963 crossrefType "journal-article" @default.
- W2125055963 hasAuthorship W2125055963A5021479204 @default.
- W2125055963 hasAuthorship W2125055963A5037204409 @default.
- W2125055963 hasAuthorship W2125055963A5053222940 @default.
- W2125055963 hasAuthorship W2125055963A5057066324 @default.
- W2125055963 hasAuthorship W2125055963A5061666247 @default.
- W2125055963 hasAuthorship W2125055963A5068318933 @default.
- W2125055963 hasAuthorship W2125055963A5083556911 @default.
- W2125055963 hasAuthorship W2125055963A5089093274 @default.
- W2125055963 hasAuthorship W2125055963A5089229546 @default.
- W2125055963 hasBestOaLocation W21250559631 @default.
- W2125055963 hasConcept C104317684 @default.
- W2125055963 hasConcept C155794727 @default.
- W2125055963 hasConcept C49105822 @default.
- W2125055963 hasConcept C54355233 @default.
- W2125055963 hasConcept C67905577 @default.
- W2125055963 hasConcept C8185291 @default.
- W2125055963 hasConcept C86803240 @default.
- W2125055963 hasConceptScore W2125055963C104317684 @default.
- W2125055963 hasConceptScore W2125055963C155794727 @default.
- W2125055963 hasConceptScore W2125055963C49105822 @default.
- W2125055963 hasConceptScore W2125055963C54355233 @default.
- W2125055963 hasConceptScore W2125055963C67905577 @default.
- W2125055963 hasConceptScore W2125055963C8185291 @default.
- W2125055963 hasConceptScore W2125055963C86803240 @default.
- W2125055963 hasIssue "4" @default.
- W2125055963 hasLocation W21250559631 @default.
- W2125055963 hasOpenAccess W2125055963 @default.
- W2125055963 hasPrimaryLocation W21250559631 @default.
- W2125055963 hasRelatedWork W2036955054 @default.
- W2125055963 hasRelatedWork W2039611121 @default.
- W2125055963 hasRelatedWork W2053368601 @default.
- W2125055963 hasRelatedWork W2060550909 @default.
- W2125055963 hasRelatedWork W2076950677 @default.
- W2125055963 hasRelatedWork W2139350041 @default.
- W2125055963 hasRelatedWork W2148003631 @default.
- W2125055963 hasRelatedWork W2154850300 @default.
- W2125055963 hasRelatedWork W2177665131 @default.
- W2125055963 hasRelatedWork W4240472420 @default.
- W2125055963 hasVolume "27" @default.
- W2125055963 isParatext "false" @default.
- W2125055963 isRetracted "false" @default.
- W2125055963 magId "2125055963" @default.
- W2125055963 workType "article" @default.