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- W2125301848 abstract "RATIONALE: The cockroach allergens that have been identified do not appear to account for the full repertoire of IgE responses. We investigated the importance of other Blattella germanica allergens that could contribute to cockroach allergy. METHODS: A B. germanica cDNA library was screened with a pool of sera from cockroach allergic patients. Three isoallergens were cloned, expressed in Pichia pastoris and purified by ion exchange and gel filtration chromatography. IgE responses to purified allergens were simultaneously measured in 104 sera using a fluorescent multiplex array system. The effect of calcium on IgE binding was investigated by ELISA. Homology modelling of the allergens was performed using Swiss-Model. RESULTS: Three isoallergens were identified and designated Bla g 6.0101, Bla g 6.0201 and Bla g 6.0301. The last two allergens shared 93 and 68% amino acid identity to Bla g 6.0101, respectively. The three isoallergens were 61-78% identical to insect troponins-C, 42-44% identical to vertebrate calmodulins, and contained two EF hand calcium binding domains. The prevalence of IgE binding to the recombinant isoallergens was ∼15%. Calcium depletion by 10mM EGTA reduced IgE binding to Bla-g-6.0101 and Bla-g-6.0301 by 40-60%. In most cases, addition of 10mM CaCl2 increased IgE binding. No effect was seen for the controls Der-p-1 and Bla-g-1. Bla-g-6 models, based on the structures of vertebrate troponin-C, showed two structurally homologous lobes mainly constituted by α-helices and loops and connected by a flexible linker. CONCLUSIONS: Bla g 6 is a minor cockroach allergen with a calcium dependent IgE reactivity, which may be involved in muscle contraction." @default.
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- W2125301848 date "2006-02-01" @default.
- W2125301848 modified "2023-09-27" @default.
- W2125301848 title "Bla g 6: A Calcium Binding German Cockroach Allergen Involved in Muscle Contraction" @default.
- W2125301848 doi "https://doi.org/10.1016/j.jaci.2005.12.470" @default.
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