Matches in SemOpenAlex for { <https://semopenalex.org/work/W2125332806> ?p ?o ?g. }
- W2125332806 endingPage "54" @default.
- W2125332806 startingPage "45" @default.
- W2125332806 abstract "The binding of low-molecular-weight heparin to an amino-terminal-truncated, 132-amino-acid, human acidic fibroblast growth factor form has been studied by isothermal titration calorimetry. This technique yields values for the enthalpy change and equilibrium constant, from which the Gibbs energy and entropy change are also calculated. Experiments in different buffers and pH values show that the protonic balance during the reaction is negligible. Experiments made at pH 7.0 with NaCl concentrations ranging from 0.20 to 0.60 M revealed changes in enthalpy and Gibbs energy in the range of -30- -17 and -27- -24 kJ x mol(-1), respectively. Isothermal titration calorimetry was also performed at different temperatures to obtain a value for the heat-capacity change at pH 7.0 and 0.4 M NaCl concentration of -96 J K- x mol(-1). A change in the length of heparin brought about no change in the thermodynamic parameters at 25 degrees C under the same experimental conditions. Changes upon ligand binding in the range of -50- -200 A2 in both polar and non-polar solvent-accessible surface areas were calculated from thermodynamic data by using different parametric equations taken from the literature. These values suggest a negligible overall conformational change in the protein when it binds to heparin and no formation of any protein-protein interface." @default.
- W2125332806 created "2016-06-24" @default.
- W2125332806 creator A5053440683 @default.
- W2125332806 creator A5058892478 @default.
- W2125332806 creator A5070228801 @default.
- W2125332806 creator A5071010355 @default.
- W2125332806 creator A5088957320 @default.
- W2125332806 date "2002-12-01" @default.
- W2125332806 modified "2023-09-25" @default.
- W2125332806 title "Energetics of heparin binding to human acidic fibroblast growth factor" @default.
- W2125332806 cites W1499963171 @default.
- W2125332806 cites W1508671389 @default.
- W2125332806 cites W1563465184 @default.
- W2125332806 cites W1591861600 @default.
- W2125332806 cites W1849896198 @default.
- W2125332806 cites W1963993009 @default.
- W2125332806 cites W1964384643 @default.
- W2125332806 cites W1964610252 @default.
- W2125332806 cites W1965127482 @default.
- W2125332806 cites W1965156165 @default.
- W2125332806 cites W1965220690 @default.
- W2125332806 cites W1975187576 @default.
- W2125332806 cites W1984302570 @default.
- W2125332806 cites W2011345555 @default.
- W2125332806 cites W2012374953 @default.
- W2125332806 cites W2013377318 @default.
- W2125332806 cites W2020357919 @default.
- W2125332806 cites W2029305252 @default.
- W2125332806 cites W2030223544 @default.
- W2125332806 cites W2030750487 @default.
- W2125332806 cites W2033702973 @default.
- W2125332806 cites W2050082215 @default.
- W2125332806 cites W2052870719 @default.
- W2125332806 cites W2063765502 @default.
- W2125332806 cites W2066936269 @default.
- W2125332806 cites W2081522451 @default.
- W2125332806 cites W2102834112 @default.
- W2125332806 cites W2117865231 @default.
- W2125332806 cites W2122892477 @default.
- W2125332806 cites W2151516954 @default.
- W2125332806 cites W2167544720 @default.
- W2125332806 cites W2171074980 @default.
- W2125332806 cites W4251144546 @default.
- W2125332806 cites W4376596386 @default.
- W2125332806 doi "https://doi.org/10.1016/s0141-8130(02)00065-x" @default.
- W2125332806 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12559426" @default.
- W2125332806 hasPublicationYear "2002" @default.
- W2125332806 type Work @default.
- W2125332806 sameAs 2125332806 @default.
- W2125332806 citedByCount "9" @default.
- W2125332806 countsByYear W21253328062013 @default.
- W2125332806 crossrefType "journal-article" @default.
- W2125332806 hasAuthorship W2125332806A5053440683 @default.
- W2125332806 hasAuthorship W2125332806A5058892478 @default.
- W2125332806 hasAuthorship W2125332806A5070228801 @default.
- W2125332806 hasAuthorship W2125332806A5071010355 @default.
- W2125332806 hasAuthorship W2125332806A5088957320 @default.
- W2125332806 hasConcept C10710636 @default.
- W2125332806 hasConcept C121332964 @default.
- W2125332806 hasConcept C146477669 @default.
- W2125332806 hasConcept C147789679 @default.
- W2125332806 hasConcept C156911925 @default.
- W2125332806 hasConcept C184866935 @default.
- W2125332806 hasConcept C185592680 @default.
- W2125332806 hasConcept C202270520 @default.
- W2125332806 hasConcept C3288061 @default.
- W2125332806 hasConcept C60205243 @default.
- W2125332806 hasConcept C97355855 @default.
- W2125332806 hasConceptScore W2125332806C10710636 @default.
- W2125332806 hasConceptScore W2125332806C121332964 @default.
- W2125332806 hasConceptScore W2125332806C146477669 @default.
- W2125332806 hasConceptScore W2125332806C147789679 @default.
- W2125332806 hasConceptScore W2125332806C156911925 @default.
- W2125332806 hasConceptScore W2125332806C184866935 @default.
- W2125332806 hasConceptScore W2125332806C185592680 @default.
- W2125332806 hasConceptScore W2125332806C202270520 @default.
- W2125332806 hasConceptScore W2125332806C3288061 @default.
- W2125332806 hasConceptScore W2125332806C60205243 @default.
- W2125332806 hasConceptScore W2125332806C97355855 @default.
- W2125332806 hasIssue "1-3" @default.
- W2125332806 hasLocation W21253328061 @default.
- W2125332806 hasLocation W21253328062 @default.
- W2125332806 hasOpenAccess W2125332806 @default.
- W2125332806 hasPrimaryLocation W21253328061 @default.
- W2125332806 hasRelatedWork W2064394461 @default.
- W2125332806 hasRelatedWork W2087359404 @default.
- W2125332806 hasRelatedWork W2125332806 @default.
- W2125332806 hasRelatedWork W2261038175 @default.
- W2125332806 hasRelatedWork W2295430905 @default.
- W2125332806 hasRelatedWork W2336584709 @default.
- W2125332806 hasRelatedWork W2386285247 @default.
- W2125332806 hasRelatedWork W2796429190 @default.
- W2125332806 hasRelatedWork W2811055826 @default.
- W2125332806 hasRelatedWork W4281566221 @default.
- W2125332806 hasVolume "31" @default.
- W2125332806 isParatext "false" @default.
- W2125332806 isRetracted "false" @default.
- W2125332806 magId "2125332806" @default.