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- W2125353358 abstract "Plants and algae contain the FtsZ1 and FtsZ2 protein families that perform specific, non-redundant functions in plastid division. In vitro studies of chloroplast division have been hampered by the lack of a suitable expression system. Here we report the expression and purification of FtsZ1-1 and FtsZ2-1 from Arabidopsis thaliana using a eukaryotic host. Specific GTPase activities were determined and found to be different for FtsZ1-1 vs. FtsZ2-1. The purified proteins readily assembled into previously unreported assembly products named type-I and -II filaments. In contrast to bacterial FtsZ, the Arabidopsis proteins do not form bundled sheets in the presence of Ca(2+)." @default.
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- W2125353358 date "2009-11-16" @default.
- W2125353358 modified "2023-09-25" @default.
- W2125353358 title "Plant FtsZ1 and FtsZ2 expressed in a eukaryotic host: GTPase activity and self-assembly" @default.
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- W2125353358 doi "https://doi.org/10.1016/j.febslet.2009.11.044" @default.
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