Matches in SemOpenAlex for { <https://semopenalex.org/work/W2125367002> ?p ?o ?g. }
- W2125367002 endingPage "2203" @default.
- W2125367002 startingPage "2191" @default.
- W2125367002 abstract "Hypoxia plays a crucial role in many pathophysiological conditions, including cancer biology, and hypoxia-inducible factor (HIF) regulates transcriptional responses under hypoxia. To elucidate the cellular responses to hypoxia, we performed chromatin immunoprecipitation with deep sequencing in combination with microarray analysis and identified HIF-1 targets. We focused on one of the novel targets, sperm-associated antigen 4 (SPAG4), whose function was unknown. SPAG4, an HIF-1-specific target, is up-regulated in various cultured cells under hypoxia. Examination of SPAG4 expression using a tissue microarray consisting of 190 human renal cell carcinoma (RCC) samples revealed that SPAG4 is an independent prognostic factor of cancer-specific mortality. Live-cell imaging revealed localization of SPAG4 at the intercellular bridge in telophase. We also studied cells in which SPAG4 was knocked down. Hypoxia enhances tetraploidy, which disturbs cell proliferation, and knockdown of SPAG4 increased tetraploid formation and decreased cell proliferation under both normoxic and hypoxic conditions. Studies using deletion mutants of SPAG4 also suggested the involvement of SPAG4 in cytokinesis. Microarray analysis confirmed dysregulation of cytokinesis-related genes by knockdown of SPAG4. In conclusion, SPAG4 is an independent prognostic factor in RCC and plays a crucial role in cytokinesis to defend against hypoxia-induced tetraploid formation. This defensive mechanism may promote survival of cancer cells under hypoxic conditions, thus leading to poor prognosis." @default.
- W2125367002 created "2016-06-24" @default.
- W2125367002 creator A5001413231 @default.
- W2125367002 creator A5005708651 @default.
- W2125367002 creator A5007057754 @default.
- W2125367002 creator A5007970106 @default.
- W2125367002 creator A5014010927 @default.
- W2125367002 creator A5020999430 @default.
- W2125367002 creator A5022342891 @default.
- W2125367002 creator A5030554556 @default.
- W2125367002 creator A5035573141 @default.
- W2125367002 creator A5036113498 @default.
- W2125367002 creator A5048262927 @default.
- W2125367002 creator A5059943171 @default.
- W2125367002 creator A5066677506 @default.
- W2125367002 creator A5066803512 @default.
- W2125367002 creator A5069750933 @default.
- W2125367002 creator A5070428340 @default.
- W2125367002 creator A5071062016 @default.
- W2125367002 creator A5090549511 @default.
- W2125367002 date "2013-06-01" @default.
- W2125367002 modified "2023-10-13" @default.
- W2125367002 title "Sperm-Associated Antigen 4, a Novel Hypoxia-Inducible Factor 1 Target, Regulates Cytokinesis, and Its Expression Correlates with the Prognosis of Renal Cell Carcinoma" @default.
- W2125367002 cites W1568426705 @default.
- W2125367002 cites W1896930635 @default.
- W2125367002 cites W1902994240 @default.
- W2125367002 cites W1965505970 @default.
- W2125367002 cites W1977922577 @default.
- W2125367002 cites W1991736202 @default.
- W2125367002 cites W1998522030 @default.
- W2125367002 cites W1998668334 @default.
- W2125367002 cites W2010749771 @default.
- W2125367002 cites W2011649455 @default.
- W2125367002 cites W2011810742 @default.
- W2125367002 cites W2014245863 @default.
- W2125367002 cites W2017575850 @default.
- W2125367002 cites W2018258698 @default.
- W2125367002 cites W2022433397 @default.
- W2125367002 cites W2027798436 @default.
- W2125367002 cites W2033731029 @default.
- W2125367002 cites W2045934831 @default.
- W2125367002 cites W2048414438 @default.
- W2125367002 cites W2063593969 @default.
- W2125367002 cites W2064980447 @default.
- W2125367002 cites W2065728197 @default.
- W2125367002 cites W2068321379 @default.
- W2125367002 cites W2069698188 @default.
- W2125367002 cites W2070413250 @default.
- W2125367002 cites W2076668534 @default.
- W2125367002 cites W2080374319 @default.
- W2125367002 cites W2082169879 @default.
- W2125367002 cites W2095341087 @default.
- W2125367002 cites W2097220641 @default.
- W2125367002 cites W2099517948 @default.
- W2125367002 cites W2102526923 @default.
- W2125367002 cites W2124111730 @default.
- W2125367002 cites W2128683837 @default.
- W2125367002 cites W2133465414 @default.
- W2125367002 cites W2139252323 @default.
- W2125367002 cites W2144938444 @default.
- W2125367002 cites W2148572322 @default.
- W2125367002 cites W2150409292 @default.
- W2125367002 cites W2156182604 @default.
- W2125367002 cites W2158217645 @default.
- W2125367002 cites W2171117253 @default.
- W2125367002 cites W2072883588 @default.
- W2125367002 doi "https://doi.org/10.1016/j.ajpath.2013.02.024" @default.
- W2125367002 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23602831" @default.
- W2125367002 hasPublicationYear "2013" @default.
- W2125367002 type Work @default.
- W2125367002 sameAs 2125367002 @default.
- W2125367002 citedByCount "26" @default.
- W2125367002 countsByYear W21253670022013 @default.
- W2125367002 countsByYear W21253670022014 @default.
- W2125367002 countsByYear W21253670022015 @default.
- W2125367002 countsByYear W21253670022016 @default.
- W2125367002 countsByYear W21253670022017 @default.
- W2125367002 countsByYear W21253670022018 @default.
- W2125367002 countsByYear W21253670022019 @default.
- W2125367002 countsByYear W21253670022020 @default.
- W2125367002 countsByYear W21253670022021 @default.
- W2125367002 countsByYear W21253670022022 @default.
- W2125367002 countsByYear W21253670022023 @default.
- W2125367002 crossrefType "journal-article" @default.
- W2125367002 hasAuthorship W2125367002A5001413231 @default.
- W2125367002 hasAuthorship W2125367002A5005708651 @default.
- W2125367002 hasAuthorship W2125367002A5007057754 @default.
- W2125367002 hasAuthorship W2125367002A5007970106 @default.
- W2125367002 hasAuthorship W2125367002A5014010927 @default.
- W2125367002 hasAuthorship W2125367002A5020999430 @default.
- W2125367002 hasAuthorship W2125367002A5022342891 @default.
- W2125367002 hasAuthorship W2125367002A5030554556 @default.
- W2125367002 hasAuthorship W2125367002A5035573141 @default.
- W2125367002 hasAuthorship W2125367002A5036113498 @default.
- W2125367002 hasAuthorship W2125367002A5048262927 @default.
- W2125367002 hasAuthorship W2125367002A5059943171 @default.
- W2125367002 hasAuthorship W2125367002A5066677506 @default.
- W2125367002 hasAuthorship W2125367002A5066803512 @default.