FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2125372209> ?p ?o ?g. }

• W2125372209 endingPage "21" @default.
• W2125372209 startingPage "14" @default.
• W2125372209 abstract "Abstract Background The fluorescent probe Syto16 has been used successfully to measure P‐glycoprotein (Pgp) function and, separately, early apoptosis and cell death. The present study was designed to evaluate whether the combined use of Syto16, the Pgp blocker PSC833, and 7‐AAD allows simultaneous detection of all parameters, with emphasis on applications in acute myeloid leukemia (AML). Methods Pgp negative/positive KB cell lines treated with tumor necrosis factor α/hyperthermia and frozen–thawed AML samples were used as apoptosis/Pgp models. Results For the accurate assessment of apoptosis in samples with unknown Pgp status, it was essential to include a sample with PSC833: in such samples, viable cells always show a Syto16 high and apoptotic cells a Syto16 low fluorescence. Apoptotic cells loose their Pgp activity early on; in Pgp‐positive cells, the Syto16 low apoptotic cells then colocalize with the Syto16 low viable cells in the situation minus PSC833. We have developed a gating strategy that, apart from quantifying apoptosis, allowed gating out these apoptotic cells for proper Pgp assessment. By using this strategy, no differences in Pgp activity were found in the treated versus the untreated samples (KB cells: P = 0.779, n = 10; AML cells: P = 0.525, n = 45). Conclusions The use of the combination Syto16/PSC833/7‐AAD provides a sensitive multiparameter flow cytometry method that enables accurate assessment of both apoptosis, cell death, and Pgp function in clinical samples. Cytometry Part B (Clin. Cytometry) 55B:14–21, 2003. © 2003 Wiley‐Liss, Inc." @default.
• W2125372209 created "2016-06-24" @default.
• W2125372209 creator A5011171028 @default.
• W2125372209 creator A5018592613 @default.
• W2125372209 creator A5069798378 @default.
• W2125372209 creator A5071977094 @default.
• W2125372209 creator A5080912335 @default.
• W2125372209 date "2003-08-22" @default.
• W2125372209 modified "2023-10-17" @default.
• W2125372209 title "Novel multiparameter flow cytometry assay using Syto16 for the simultaneous detection of early apoptosis and apoptosis‐corrected P‐glycoprotein function in clinical samples" @default.
• W2125372209 cites W102593176 @default.
• W2125372209 cites W1490418776 @default.
• W2125372209 cites W1965565653 @default.
• W2125372209 cites W1966101727 @default.
• W2125372209 cites W1972150957 @default.
• W2125372209 cites W1975785759 @default.
• W2125372209 cites W1983266647 @default.
• W2125372209 cites W1990309210 @default.
• W2125372209 cites W1991253583 @default.
• W2125372209 cites W1994700879 @default.
• W2125372209 cites W2013194808 @default.
• W2125372209 cites W2013995823 @default.
• W2125372209 cites W2038512880 @default.
• W2125372209 cites W2052804212 @default.
• W2125372209 cites W2053380672 @default.
• W2125372209 cites W2054787711 @default.
• W2125372209 cites W2057772226 @default.
• W2125372209 cites W2058108603 @default.
• W2125372209 cites W2060274926 @default.
• W2125372209 cites W2065201141 @default.
• W2125372209 cites W2071025146 @default.
• W2125372209 cites W2076750960 @default.
• W2125372209 cites W2078532650 @default.
• W2125372209 cites W2081641246 @default.
• W2125372209 cites W2138265677 @default.
• W2125372209 cites W2167564928 @default.
• W2125372209 cites W2319622120 @default.
• W2125372209 cites W3149097951 @default.
• W2125372209 doi "https://doi.org/10.1002/cyto.b.10024" @default.
• W2125372209 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12949955" @default.
• W2125372209 hasPublicationYear "2003" @default.
• W2125372209 type Work @default.
• W2125372209 sameAs 2125372209 @default.
• W2125372209 citedByCount "33" @default.
• W2125372209 countsByYear W21253722092012 @default.
• W2125372209 countsByYear W21253722092013 @default.
• W2125372209 countsByYear W21253722092014 @default.
• W2125372209 countsByYear W21253722092015 @default.
• W2125372209 countsByYear W21253722092019 @default.
• W2125372209 countsByYear W21253722092022 @default.
• W2125372209 crossrefType "journal-article" @default.
• W2125372209 hasAuthorship W2125372209A5011171028 @default.
• W2125372209 hasAuthorship W2125372209A5018592613 @default.
• W2125372209 hasAuthorship W2125372209A5069798378 @default.
• W2125372209 hasAuthorship W2125372209A5071977094 @default.
• W2125372209 hasAuthorship W2125372209A5080912335 @default.
• W2125372209 hasBestOaLocation W21253722091 @default.
• W2125372209 hasConcept C133936738 @default.
• W2125372209 hasConcept C153911025 @default.
• W2125372209 hasConcept C190283241 @default.
• W2125372209 hasConcept C2778707650 @default.
• W2125372209 hasConcept C2780339063 @default.
• W2125372209 hasConcept C31573885 @default.
• W2125372209 hasConcept C501593827 @default.
• W2125372209 hasConcept C502942594 @default.
• W2125372209 hasConcept C553184892 @default.
• W2125372209 hasConcept C55493867 @default.
• W2125372209 hasConcept C86803240 @default.
• W2125372209 hasConceptScore W2125372209C133936738 @default.
• W2125372209 hasConceptScore W2125372209C153911025 @default.
• W2125372209 hasConceptScore W2125372209C190283241 @default.
• W2125372209 hasConceptScore W2125372209C2778707650 @default.
• W2125372209 hasConceptScore W2125372209C2780339063 @default.
• W2125372209 hasConceptScore W2125372209C31573885 @default.
• W2125372209 hasConceptScore W2125372209C501593827 @default.
• W2125372209 hasConceptScore W2125372209C502942594 @default.
• W2125372209 hasConceptScore W2125372209C553184892 @default.
• W2125372209 hasConceptScore W2125372209C55493867 @default.
• W2125372209 hasConceptScore W2125372209C86803240 @default.
• W2125372209 hasIssue "1" @default.
• W2125372209 hasLocation W21253722091 @default.
• W2125372209 hasLocation W21253722092 @default.
• W2125372209 hasOpenAccess W2125372209 @default.
• W2125372209 hasPrimaryLocation W21253722091 @default.
• W2125372209 hasRelatedWork W2030130210 @default.
• W2125372209 hasRelatedWork W2160904476 @default.
• W2125372209 hasRelatedWork W2176701861 @default.
• W2125372209 hasRelatedWork W2253642286 @default.
• W2125372209 hasRelatedWork W2351699250 @default.
• W2125372209 hasRelatedWork W2361962797 @default.
• W2125372209 hasRelatedWork W2361980913 @default.
• W2125372209 hasRelatedWork W2363178760 @default.
• W2125372209 hasRelatedWork W3009159775 @default.
• W2125372209 hasRelatedWork W4251194061 @default.
• W2125372209 hasVolume "55B" @default.
• W2125372209 isParatext "false" @default.
• W2125372209 isRetracted "false" @default.
• W2125372209 magId "2125372209" @default.

• next