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- W2125461580 abstract "In Tetrahymena thermophila, peptides secreted via dense-core granules, called mucocysts, are generated by proprotein processing. We used expression profiling to identify candidate processing enzymes, which localized as cyan fluorescent protein fusions to mucocysts. Of note, the aspartyl cathepsin Cth3p plays a key role in mucocyst-based secretion, since knockdown of this gene blocked proteolytic maturation of the entire set of mucocyst proproteins and dramatically reduced mucocyst accumulation. The activity of Cth3p was eliminated by mutation of two predicted active-site mutations, and overexpression of the wild-type gene, but not the catalytic-site mutant, partially rescued a Mendelian mutant defective in mucocyst proprotein processing. Our results provide the first direct evidence for the role of proprotein processing in this system. Of interest, both localization and the CTH3 disruption phenotype suggest that the enzyme provides non–mucocyst-related functions. Phylogenetic analysis of the T. thermophila cathepsins, combined with prior work on the role of sortilin receptors in mucocyst biogenesis, suggests that repurposing of lysosomal enzymes was an important step in the evolution of secretory granules in ciliates." @default.
- W2125461580 created "2016-06-24" @default.
- W2125461580 creator A5041970526 @default.
- W2125461580 creator A5050000749 @default.
- W2125461580 creator A5080678140 @default.
- W2125461580 date "2014-08-15" @default.
- W2125461580 modified "2023-09-26" @default.
- W2125461580 title "An aspartyl cathepsin,<i>CTH3</i>, is essential for proprotein processing during secretory granule maturation in<i>Tetrahymena thermophila</i>" @default.
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- W2125461580 doi "https://doi.org/10.1091/mbc.e14-03-0833" @default.
- W2125461580 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4142616" @default.
- W2125461580 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24943840" @default.
- W2125461580 hasPublicationYear "2014" @default.
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