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- W2125536596 endingPage "229" @default.
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- W2125536596 abstract "We report, exogenous addition of ceramide significantly increases 12-hydroxyeicosatetraenoic acid [12-(S)-HETE] levels, in a dose-dependent manner. 12-(S)-HETE levels, in 20, 30 and 40 μM ceramide exposed rat primary hippocampal cell cultures containing predominantly astrocytes and few neurons and other glial cells (the cultured hippocampal cells were predominantly astrocytes amounting to over 99% of total cells with few neurons and other glial cells) amounted to 207, 260 and 408% of the controls, respectively. However, dihydroceramide, an inactive analog of ceramide did not alter the levels of 12-(S)-HETE. Ceramide also increased the mRNA and protein expression, and activity of 12-lipoxygease (12-LOX) needed for the synthesis of 12(S)-HETE. These results indicate a possible link between ceramide and 12-LOX pathway. However, ceramide did not alter expression of 5-lipoxygenase (5-LOX), another member of the lipoxygenase family. However, ceramide upregulated expression of cytosolic phospholipase-A2 (cPLA2) and cyclooxygenase-2 (COX-2). Further, ceramide caused a significant increase in the levels of reactive oxygen species (ROS). Ceramide-mediated generation of ROS was inhibited by baicalien but not by indomethacin. In addition, ceramide treated cells exhibited increased mRNA expression of DNA damage induced transcript3 (Ddit3). This report which demonstrate induction of pro-carcinogenic 12-LOX pathway by an anticancer ceramide, may be relevant to cancer biologists studying drug resistant tumors and devising potent anticancer therapeutic strategies to treat drug resistant tumors. These results indicate possibility of 12-LOX involvement in ceramide-mediated generation of ROS and cellular oxidative stress. Induction of 12-LOX pathway by ceramide may have implications in understanding pathophysiology of neurodegenerative diseases involving ROS generation and inflammation." @default.
- W2125536596 created "2016-06-24" @default.
- W2125536596 creator A5008000109 @default.
- W2125536596 creator A5053222370 @default.
- W2125536596 creator A5086278625 @default.
- W2125536596 date "2008-12-01" @default.
- W2125536596 modified "2023-10-16" @default.
- W2125536596 title "Ceramide elevates 12-hydroxyeicosatetraenoic acid levels and upregulates 12-lipoxygenase in rat primary hippocampal cell cultures containing predominantly astrocytes" @default.
- W2125536596 cites W1529434345 @default.
- W2125536596 cites W1536767595 @default.
- W2125536596 cites W1540472115 @default.
- W2125536596 cites W1547508081 @default.
- W2125536596 cites W1567681172 @default.
- W2125536596 cites W1576988825 @default.
- W2125536596 cites W1735728105 @default.
- W2125536596 cites W1843461492 @default.
- W2125536596 cites W1870141733 @default.
- W2125536596 cites W1905538010 @default.
- W2125536596 cites W1964545403 @default.
- W2125536596 cites W1967042188 @default.
- W2125536596 cites W1976168084 @default.
- W2125536596 cites W1977644244 @default.
- W2125536596 cites W1978813781 @default.
- W2125536596 cites W1979536578 @default.
- W2125536596 cites W1981947454 @default.
- W2125536596 cites W1982533264 @default.
- W2125536596 cites W1983305695 @default.
- W2125536596 cites W1983346143 @default.
- W2125536596 cites W1985265962 @default.
- W2125536596 cites W1985818898 @default.
- W2125536596 cites W1987621344 @default.
- W2125536596 cites W1987856385 @default.
- W2125536596 cites W1990368145 @default.
- W2125536596 cites W1994662389 @default.
- W2125536596 cites W1995221636 @default.
- W2125536596 cites W1995783979 @default.
- W2125536596 cites W1998702692 @default.
- W2125536596 cites W1998796501 @default.
- W2125536596 cites W1999160838 @default.
- W2125536596 cites W2000684668 @default.
- W2125536596 cites W2001125080 @default.
- W2125536596 cites W2001454826 @default.
- W2125536596 cites W2001649345 @default.
- W2125536596 cites W2003302931 @default.
- W2125536596 cites W2005071794 @default.
- W2125536596 cites W2006216249 @default.
- W2125536596 cites W2008590915 @default.
- W2125536596 cites W2011716403 @default.
- W2125536596 cites W2012660133 @default.
- W2125536596 cites W2014923090 @default.
- W2125536596 cites W2017191741 @default.
- W2125536596 cites W2017354884 @default.
- W2125536596 cites W2017998100 @default.
- W2125536596 cites W2018548034 @default.
- W2125536596 cites W2019095153 @default.
- W2125536596 cites W2020014389 @default.
- W2125536596 cites W2023599526 @default.
- W2125536596 cites W2025622702 @default.
- W2125536596 cites W2027619123 @default.
- W2125536596 cites W2028434936 @default.
- W2125536596 cites W2028652974 @default.
- W2125536596 cites W2029315686 @default.
- W2125536596 cites W2029530057 @default.
- W2125536596 cites W2030639205 @default.
- W2125536596 cites W2031983485 @default.
- W2125536596 cites W2032315776 @default.
- W2125536596 cites W2037112744 @default.
- W2125536596 cites W2039050258 @default.
- W2125536596 cites W2039400966 @default.
- W2125536596 cites W2039531779 @default.
- W2125536596 cites W2041241051 @default.
- W2125536596 cites W2041619581 @default.
- W2125536596 cites W2041860844 @default.
- W2125536596 cites W2042789574 @default.
- W2125536596 cites W2044122230 @default.
- W2125536596 cites W2044236402 @default.
- W2125536596 cites W2044740167 @default.
- W2125536596 cites W2045881139 @default.
- W2125536596 cites W2048619099 @default.
- W2125536596 cites W2052271156 @default.
- W2125536596 cites W2052938737 @default.
- W2125536596 cites W2053869318 @default.
- W2125536596 cites W2055063817 @default.
- W2125536596 cites W2055329023 @default.
- W2125536596 cites W2055340939 @default.
- W2125536596 cites W2056402816 @default.
- W2125536596 cites W2061511599 @default.
- W2125536596 cites W2062051904 @default.
- W2125536596 cites W2062188366 @default.
- W2125536596 cites W2062780075 @default.
- W2125536596 cites W2065766712 @default.
- W2125536596 cites W2067496178 @default.
- W2125536596 cites W2067674980 @default.
- W2125536596 cites W2069924169 @default.
- W2125536596 cites W2070620348 @default.
- W2125536596 cites W2073754684 @default.
- W2125536596 cites W2074144178 @default.
- W2125536596 cites W2075286695 @default.