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• W2125717542 endingPage "1556" @default.
• W2125717542 startingPage "1548" @default.
• W2125717542 abstract "The cyclic AMP (cAMP)-protein kinase A (PKA) signaling activates virulence expression during hyphal development in the fungal human pathogen Candida albicans. The hyphal growth is characterized by Golgi polarization toward the hyphal tips, which is thought to enhance directional vesicle transport. However, how the hypha-induction signal regulates Golgi polarization is unknown. Gyp1, a Golgi-associated protein and the first GTPase-activating protein (GAP) in the Rab GAP cascade, critically regulates membrane trafficking from the endoplasmic reticulum to the plasma membrane. Here, we report a novel pathway by which the cAMP-PKA signaling triggers Golgi polarization during hyphal growth. We demonstrate that Gyp1 plays a crucial role in actin-dependent Golgi polarization. Hyphal induction activates PKA, which in turn phosphorylates Gyp1. Phosphomimetic mutation of four PKA sites identified by mass spectrometry (Gyp1(4E)) caused strong Gyp1 polarization to hyphal tips, whereas nonphosphorylatable mutations (Gyp1(4A)) abolished it. Gyp1(4E) exhibited enhanced association with the actin motor Myo2, while Gyp1(4A) showed the opposite effect, providing a possible mechanism for Golgi polarization. A GAP-dead Gyp1 (Gyp1(R292K)) showed strong polarization similar to that seen with Gyp1(4E), indicating a role for the GAP activity. Mutating the PKA sites on Gyp1 also impaired the recruitment of a late Golgi marker, Sec7. Furthermore, proper PKA phosphorylation and GAP activity of Gyp1 are required for virulence in mice. We propose that the cAMP-PKA signaling directly targets Gyp1 to promote Golgi polarization in the yeast-to-hypha transition, an event crucial for C. albicans infection." @default.
• W2125717542 created "2016-06-24" @default.
• W2125717542 creator A5033632697 @default.
• W2125717542 creator A5044620494 @default.
• W2125717542 creator A5056381737 @default.
• W2125717542 creator A5087404367 @default.
• W2125717542 date "2014-12-01" @default.
• W2125717542 modified "2023-10-12" @default.
• W2125717542 title "Novel Mechanism Coupling Cyclic AMP-Protein Kinase A Signaling and Golgi Trafficking via Gyp1 Phosphorylation in Polarized Growth" @default.
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• W2125717542 doi "https://doi.org/10.1128/ec.00231-14" @default.
• W2125717542 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4248690" @default.
• W2125717542 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25326521" @default.
• W2125717542 hasPublicationYear "2014" @default.
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