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- W2125893251 abstract "Abstract Metarhizium anisopliae spores were produced on nutrient‐impregnated membranes (NIMs). The NIM system involved wetting the membrane with a spore and nutrient suspension, followed by harvesting the spores produced after incubation. The cost efficiency of spore production was assessed for a range of nutrient sources and membrane types. Skim milk powder (20 g l‐1) was found to be the most cost‐effective nutrient source of the nine nutrients examined. Yield was 5.7 × 106 spores/cm2 after 28 days incubation on a paper membrane. Supplementation of the skim milk with either sucrose (2 g l‐1) or dextrose + KNO3 maximized yield. Superwipe, an absorbent fibrous material, was the most efficient of 16 membranes tested which ranged from fibreglass mesh to paper and cloth. A series of small pilot plants were built, but the cost efficiency of spore production decreased as the size of the membrane increased from 24 × 24 cm to 270 × 15 cm and up to 100 × 80 cm. Yield on the two smaller pilot plants was over 107spores/cm2, but the cost (nutrient and membrane only) of producing 1013 spores (standard dose required per hectare) was around $A37 and was found not to be competitive with spore production on grain. Keywords: Metarhizium anisopliaespore productionconidiabiological controlcostsshelf‐life" @default.
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- W2125893251 title "Production of<i>Metarhizium anisopliae</i>spores using nutrient‐impregnated membranes and its economic analysis" @default.
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- W2125893251 doi "https://doi.org/10.1080/09583159409355338" @default.
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