FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2125987764> ?p ?o ?g. }

• W2125987764 abstract "1. The abilities of the four diastereoisomers of 1-aminocyclopentane-1,3-dicarboxylic acid (ACPD) to stimulate, and the metabotropic glutamate receptor (mGluR) antagonist (+/-)-alpha-methylcarboxyphenylglycine (MCPG) to inhibit, phosphoinositide turnover in neonatal rat cerebral cortex have been studied. Two indices of phosphoinositide cycle activity were assessed; inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) mass accumulation, and total inositol phosphate [3H]-InsPx accumulation (in the presence of Li+) in myo-[3H]-inositol prelabelled slices. 2. The diastereoisomers of ACPD stimulated each response with a rank order of potency of 1S, 3R > 1R, 3R > 1S, 3S >> 1R, 3S. The response to 1R, 3R-ACPD was largely prevented by pre-addition of the NMDA-receptor antagonist, MK-801, or omission of extracellular Ca2+, suggesting that this isomer acts indirectly on phosphoinositide responses through activation of NMDA-type ionotropic glutamate receptors. In contrast, the responses to 1S, 3R- and 1S, 3S-ACPD were unaffected by prior addition of MK-801, but were blocked by MCPG. 3. The concentration of 1S, 3R-ACPD required to half-maximally stimulate the Ins(1,4,5)P3 response (-log EC50 (M), -4.09 +/- 0.10) was significantly higher than that required to exert a similar effect on [3H]-InsPx accumulation (-log EC50 (M), -4.87 +/- 0.07; P < 0.01; n = 4). A similar marked 8-9 fold discrepancy between these two values was observed for the 1S, 3S isomer, which elicited similar maximal responses to those caused by 1S, 3R-ACPD. 4. Significant differences were also observed with respect to the ability of (+/-)-MCPG (1 mM) to cause a rightward shift in the concentration-response relationships for 1S, 3R-ACPD-stimulated Ins(1,4,5)P3 (5.59 +/- 0.24 fold shift) and [3H]-InsPx (3.04 +/- 0.34 fold shift; P < 0.01; n = 4) responses, giving rise to Kd values of 218 and 490 microM for (+/-)-MCPG antagonism of the respective responses. 5. The potency difference between the 1S, 3R-ACPD-stimulated Ins(1,4,5)P3 and [3H]-InsPx responses was reduced when experiments were performed in nominally calcium-free medium ([Ca2+]e = 2 - 5 microM) and EC50 values were almost identical when extracellular calcium was reduced further by EGTA addition ([Ca2+]e < or = 100 nM). Similarly, the Kd value for (+/-)-MCPG antagonism of the 1S, 3R-ACPD-stimulated [3H]-InsPx response decreased under [Ca2+]e-free conditions, approaching those obtained for the 1S, 3R-ACPD-stimulated Ins(1,4,5)P3 response in the presence of normal [Ca2+]e. 6. These data suggest that estimates of the activities of mGluR agonists and antagonists, derived by measuring phosphoinositide turnover, can differ significantly depending on whether Ins(1,4,5)P3 mass or [3H]-InsPx responses are measured. In particular, the possibility that the mGluR-mediated [3H]-InsPx response may not simply reflect direct receptor/G protein/phosphoinositidase C (PIC) activation, but may also be the consequence of stimulation of a facilitatory Ca2+-influx pathway is discussed." @default.
• W2125987764 created "2016-06-24" @default.
• W2125987764 creator A5044018474 @default.
• W2125987764 creator A5062164148 @default.
• W2125987764 date "1996-04-01" @default.
• W2125987764 modified "2023-10-14" @default.
• W2125987764 title "Differences in agonist and antagonist activities for two indices of metabotropic glutamate receptor-stimulated phosphoinositide turnover" @default.
• W2125987764 cites W1509785349 @default.
• W2125987764 cites W1563289295 @default.
• W2125987764 cites W1775749144 @default.
• W2125987764 cites W1825542739 @default.
• W2125987764 cites W1851047213 @default.
• W2125987764 cites W1948280938 @default.
• W2125987764 cites W1950417202 @default.
• W2125987764 cites W1981115167 @default.
• W2125987764 cites W1982340263 @default.
• W2125987764 cites W1992347253 @default.
• W2125987764 cites W1992904711 @default.
• W2125987764 cites W1995243710 @default.
• W2125987764 cites W1999508322 @default.
• W2125987764 cites W2003760589 @default.
• W2125987764 cites W2007870596 @default.
• W2125987764 cites W2011114281 @default.
• W2125987764 cites W2016233464 @default.
• W2125987764 cites W2018137034 @default.
• W2125987764 cites W2022975988 @default.
• W2125987764 cites W2035115219 @default.
• W2125987764 cites W2043166209 @default.
• W2125987764 cites W2046208200 @default.
• W2125987764 cites W2047107569 @default.
• W2125987764 cites W2048376323 @default.
• W2125987764 cites W2050419480 @default.
• W2125987764 cites W2058205481 @default.
• W2125987764 cites W2059501084 @default.
• W2125987764 cites W2065166861 @default.
• W2125987764 cites W2066439059 @default.
• W2125987764 cites W2066935607 @default.
• W2125987764 cites W2068761941 @default.
• W2125987764 cites W2078935283 @default.
• W2125987764 cites W2085210285 @default.
• W2125987764 cites W2086894916 @default.
• W2125987764 cites W2088301595 @default.
• W2125987764 cites W2090006256 @default.
• W2125987764 cites W2095239151 @default.
• W2125987764 cites W2121431393 @default.
• W2125987764 cites W2137376734 @default.
• W2125987764 cites W2140142911 @default.
• W2125987764 cites W2161584310 @default.
• W2125987764 cites W2432868173 @default.
• W2125987764 cites W2471637005 @default.
• W2125987764 doi "https://doi.org/10.1111/j.1476-5381.1996.tb15347.x" @default.
• W2125987764 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1909577" @default.
• W2125987764 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8732284" @default.
• W2125987764 hasPublicationYear "1996" @default.
• W2125987764 type Work @default.
• W2125987764 sameAs 2125987764 @default.
• W2125987764 citedByCount "7" @default.
• W2125987764 countsByYear W21259877642014 @default.
• W2125987764 countsByYear W21259877642015 @default.
• W2125987764 crossrefType "journal-article" @default.
• W2125987764 hasAuthorship W2125987764A5044018474 @default.
• W2125987764 hasAuthorship W2125987764A5062164148 @default.
• W2125987764 hasBestOaLocation W21259877641 @default.
• W2125987764 hasConcept C115955781 @default.
• W2125987764 hasConcept C126322002 @default.
• W2125987764 hasConcept C134018914 @default.
• W2125987764 hasConcept C170493617 @default.
• W2125987764 hasConcept C185592680 @default.
• W2125987764 hasConcept C26702167 @default.
• W2125987764 hasConcept C2776885963 @default.
• W2125987764 hasConcept C2777427919 @default.
• W2125987764 hasConcept C2777603759 @default.
• W2125987764 hasConcept C2778618036 @default.
• W2125987764 hasConcept C2778938600 @default.
• W2125987764 hasConcept C49051014 @default.
• W2125987764 hasConcept C55493867 @default.
• W2125987764 hasConcept C61174792 @default.
• W2125987764 hasConcept C67018056 @default.
• W2125987764 hasConcept C71924100 @default.
• W2125987764 hasConcept C86803240 @default.
• W2125987764 hasConceptScore W2125987764C115955781 @default.
• W2125987764 hasConceptScore W2125987764C126322002 @default.
• W2125987764 hasConceptScore W2125987764C134018914 @default.
• W2125987764 hasConceptScore W2125987764C170493617 @default.
• W2125987764 hasConceptScore W2125987764C185592680 @default.
• W2125987764 hasConceptScore W2125987764C26702167 @default.
• W2125987764 hasConceptScore W2125987764C2776885963 @default.
• W2125987764 hasConceptScore W2125987764C2777427919 @default.
• W2125987764 hasConceptScore W2125987764C2777603759 @default.
• W2125987764 hasConceptScore W2125987764C2778618036 @default.
• W2125987764 hasConceptScore W2125987764C2778938600 @default.
• W2125987764 hasConceptScore W2125987764C49051014 @default.
• W2125987764 hasConceptScore W2125987764C55493867 @default.
• W2125987764 hasConceptScore W2125987764C61174792 @default.
• W2125987764 hasConceptScore W2125987764C67018056 @default.
• W2125987764 hasConceptScore W2125987764C71924100 @default.
• W2125987764 hasConceptScore W2125987764C86803240 @default.
• W2125987764 hasLocation W21259877641 @default.
• W2125987764 hasLocation W21259877642 @default.
• W2125987764 hasLocation W21259877643 @default.

• next