Matches in SemOpenAlex for { <https://semopenalex.org/work/W2126075306> ?p ?o ?g. }
- W2126075306 endingPage "265" @default.
- W2126075306 startingPage "255" @default.
- W2126075306 abstract "The exit (E) site has been implicated in several ribosomal activities, including translocation, decoding, and maintenance of the translational reading frame. Here, we target the 30S subunit E site by introducing a deletion in rpsG that truncates the β-hairpin of ribosomal protein S7. This mutation (S7ΔR77–Y84) increases both −1 and +1 frameshifting but does not increase miscoding, providing evidence that the 30S E site plays a specific role in frame maintenance. Mutation S7ΔR77–Y84 also stimulates +1 programmed frameshifting during prfB′-lacZ translation in many synthetic contexts. However, no effect is seen when the E codon of the frameshift site corresponds to those found in nature, suggesting that E-tRNA release does not normally limit the rate of prfB frameshifting. Ribosomes containing S7ΔR77–Y84 exhibit an elevated rate of spontaneous reverse translocation and an increased K 1/2 for E-tRNA. These effects are of similar magnitude, suggesting that both result from destabilization of E-tRNA. Finally, this mutation of the 30S E site does not inhibit EF-G-dependent translocation, consistent with a primary role for the 50S E site in the mechanism." @default.
- W2126075306 created "2016-06-24" @default.
- W2126075306 creator A5031416408 @default.
- W2126075306 creator A5036392168 @default.
- W2126075306 creator A5043010132 @default.
- W2126075306 creator A5054018140 @default.
- W2126075306 date "2008-12-17" @default.
- W2126075306 modified "2023-10-01" @default.
- W2126075306 title "A role for the 30S subunit E site in maintenance of the translational reading frame" @default.
- W2126075306 cites W1483334916 @default.
- W2126075306 cites W1557848130 @default.
- W2126075306 cites W1974100775 @default.
- W2126075306 cites W1975085160 @default.
- W2126075306 cites W1976067034 @default.
- W2126075306 cites W1976328208 @default.
- W2126075306 cites W1986381105 @default.
- W2126075306 cites W1994587909 @default.
- W2126075306 cites W2001660333 @default.
- W2126075306 cites W2007242362 @default.
- W2126075306 cites W201174390 @default.
- W2126075306 cites W2012983065 @default.
- W2126075306 cites W2022754910 @default.
- W2126075306 cites W2031580496 @default.
- W2126075306 cites W2038864352 @default.
- W2126075306 cites W2039557462 @default.
- W2126075306 cites W2042700625 @default.
- W2126075306 cites W2050329965 @default.
- W2126075306 cites W2051449352 @default.
- W2126075306 cites W2052163027 @default.
- W2126075306 cites W2053520514 @default.
- W2126075306 cites W2054511300 @default.
- W2126075306 cites W2054685372 @default.
- W2126075306 cites W2062997771 @default.
- W2126075306 cites W2068720171 @default.
- W2126075306 cites W2072710015 @default.
- W2126075306 cites W2077382897 @default.
- W2126075306 cites W2078079856 @default.
- W2126075306 cites W2080720176 @default.
- W2126075306 cites W2080739894 @default.
- W2126075306 cites W2081667755 @default.
- W2126075306 cites W2082475735 @default.
- W2126075306 cites W2083147729 @default.
- W2126075306 cites W2086899951 @default.
- W2126075306 cites W2093041571 @default.
- W2126075306 cites W2093253527 @default.
- W2126075306 cites W2093351149 @default.
- W2126075306 cites W2093471887 @default.
- W2126075306 cites W2094811968 @default.
- W2126075306 cites W2095598198 @default.
- W2126075306 cites W2099029809 @default.
- W2126075306 cites W2104604761 @default.
- W2126075306 cites W2105212067 @default.
- W2126075306 cites W2107447510 @default.
- W2126075306 cites W2114403312 @default.
- W2126075306 cites W2123934971 @default.
- W2126075306 cites W2125957234 @default.
- W2126075306 cites W2137206793 @default.
- W2126075306 cites W2141997506 @default.
- W2126075306 cites W2152001964 @default.
- W2126075306 cites W2153540299 @default.
- W2126075306 cites W2158157849 @default.
- W2126075306 cites W2171933385 @default.
- W2126075306 cites W32286231 @default.
- W2126075306 doi "https://doi.org/10.1261/rna.1320109" @default.
- W2126075306 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2648707" @default.
- W2126075306 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19095617" @default.
- W2126075306 hasPublicationYear "2008" @default.
- W2126075306 type Work @default.
- W2126075306 sameAs 2126075306 @default.
- W2126075306 citedByCount "63" @default.
- W2126075306 countsByYear W21260753062012 @default.
- W2126075306 countsByYear W21260753062013 @default.
- W2126075306 countsByYear W21260753062014 @default.
- W2126075306 countsByYear W21260753062015 @default.
- W2126075306 countsByYear W21260753062016 @default.
- W2126075306 countsByYear W21260753062017 @default.
- W2126075306 countsByYear W21260753062018 @default.
- W2126075306 countsByYear W21260753062019 @default.
- W2126075306 countsByYear W21260753062020 @default.
- W2126075306 countsByYear W21260753062021 @default.
- W2126075306 countsByYear W21260753062022 @default.
- W2126075306 countsByYear W21260753062023 @default.
- W2126075306 crossrefType "journal-article" @default.
- W2126075306 hasAuthorship W2126075306A5031416408 @default.
- W2126075306 hasAuthorship W2126075306A5036392168 @default.
- W2126075306 hasAuthorship W2126075306A5043010132 @default.
- W2126075306 hasAuthorship W2126075306A5054018140 @default.
- W2126075306 hasBestOaLocation W21260753061 @default.
- W2126075306 hasConcept C104317684 @default.
- W2126075306 hasConcept C105580179 @default.
- W2126075306 hasConcept C106774688 @default.
- W2126075306 hasConcept C107824862 @default.
- W2126075306 hasConcept C149364088 @default.
- W2126075306 hasConcept C153957851 @default.
- W2126075306 hasConcept C166253671 @default.
- W2126075306 hasConcept C167625842 @default.
- W2126075306 hasConcept C29906990 @default.
- W2126075306 hasConcept C47289529 @default.