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• W2126084722 abstract "No AccessJournal of UrologyAdult urology1 Jun 2006Multiplex Polymerase Chain Reaction for Microsatellite Analysis of Urine Sediment Cells: A Rapid and Inexpensive Method for Diagnosing and Monitoring Superficial Transitional Bladder Cell Carcinomais accompanied byEvaluation of Survivin Reverse Transcriptase-Polymerase Chain Reaction for Noninvasive Detection of Bladder Cancer Riccardo Bartoletti, Tommaso Cai, Maurizio Dal Canto, Vieri Boddi, Gabriella Nesi, and Mauro Piazzini Riccardo BartolettiRiccardo Bartoletti Department of Urology, University of Florence, Florence, Italy More articles by this author , Tommaso CaiTommaso Cai Department of Urology, University of Florence, Florence, Italy More articles by this author , Maurizio Dal CantoMaurizio Dal Canto Department of Urology, University of Florence, Florence, Italy More articles by this author , Vieri BoddiVieri Boddi Department of Public Health and Epidemiology, University of Florence, Florence, Italy More articles by this author , Gabriella NesiGabriella Nesi Department of Pathology, University of Florence, Florence, Italy More articles by this author , and Mauro PiazziniMauro Piazzini Medical Genetics, University of Florence, Florence, Italy More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(06)00283-7AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Several urinary markers have been recently introduced in clinical practice for improving the noninvasive diagnosis of transitional cell carcinoma. Although microsatellite analysis must be considered the best method in terms of results, its cost and method time are unacceptable for daily use. We validated a more rapid and inexpensive method of determination using rapid DNA extraction and automatic multiplex polymerase chain reaction amplification. Materials and Methods: A total of 120 patients who presented consecutively to a urological office, including 73 with transitional cell carcinoma and 43 who served as controls, were selected for study. Microsatellite analysis was performed in the blood/urine pair using 3 multiplex polymerase chain reactions per patient. Urine sediment inflammatory cells were assessed by urine dipstick test. Ten microsatellite loci were investigated. Numerical data collected during electrophoresis of the amplified segment in an ABI Prism® 310 Genetic Analyzer were used to calculate the cutoff for allelic imbalance. Method sensitivity, specificity, and positive and negative predictive values were calculated. Results: A total of 66 patients had microsatellite analysis alterations in urine sediment, of whom 59 had transitional cell carcinoma, while 7 had other urological diseases. Test sensitivity and specificity were 80.8% and 85.1%, respectively. Statistical analysis did not indicate any significant influence of inflammatory status on microsatellite analysis diagnostic performance. In the control group the allelic imbalance on chromosome 9 was significantly lower than on other chromosomes (p = 0.0143). This could confirm that chromosome 9 has a specific role in transitional cell carcinoma. The multiplex microsatellite analysis method was low cost and not time-consuming. Conclusions: Multiplex microsatellite analysis is a noninvasive, rapid, inexpensive and reproducible method for screening for and monitoring superficial transitional cell carcinoma. It should be considered an alternative method to urinary cytology and it should also be considered in the presence of urine sediment inflammatory cells. References 1 : Urine based markers of urological malignancy. J Urol2001; 165: 600. Link, Google Scholar 2 : Microsatellite analysis of urine sediment versus urine cytology for diagnosing transitional cell tumors of the urinary bladder. APMIS2004; 112: 148. Google Scholar 3 : Role of uCyt+ in the detection and surveillance of urothelial carcinoma. Urology2003; 61: 243. Google Scholar 4 : Exclusion criteria enhance the specificity and positive predictive value of NMP22 and BTA stat. J Urol1999; 162: 53. Link, Google Scholar 5 : Cytology, flow cytometry, image analysis, and interphase cytogenetics by fluorescence in situ hybridization in the diagnosis of transitional cell carcinoma in bladder washes: a comparative study. Diagn Cytopathol1995; 13: 214. 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Google Scholar © 2006 by American Urological AssociationFiguresReferencesRelatedDetailsCited byRosser C, Ross S, Chang M, Dai Y, Mengual L, Zhang G, Kim J, Urquidi V, Alcaraz A and Goodison S (2018) Multiplex Protein Signature for the Detection of Bladder Cancer in Voided Urine SamplesJournal of Urology, VOL. 190, NO. 6, (2257-2262), Online publication date: 1-Dec-2013.Cai T, Nesi G, Dal Canto M, Tinacci G, Mondaini N, Piazzini M, Geppetti P and Bartoletti R (2018) Loss of Heterozygosis on IFN-α Locus is a Prognostic Indicator of Bacillus Calmette-Guerin Response for Nonmuscle Invasive Bladder CancerJournal of Urology, VOL. 183, NO. 5, (1738-1743), Online publication date: 1-May-2010.Cai T, Mazzoli S, Meacci F, Tinacci G, Nesi G, Zini E and Bartoletti R (2018) Interleukin-6/10 Ratio as a Prognostic Marker of Recurrence in Patients With Intermediate Risk Urothelial Bladder CarcinomaJournal of Urology, VOL. 178, NO. 5, (1906-1912), Online publication date: 1-Nov-2007.Related articlesJournal of Urology9 Nov 2018Evaluation of Survivin Reverse Transcriptase-Polymerase Chain Reaction for Noninvasive Detection of Bladder Cancer Volume 175Issue 6June 2006Page: 2032-2037 Advertisement Copyright & Permissions© 2006 by American Urological AssociationKeywordscarcinomaloss of heterozygositygenomic instabilitybladdermicrosatellite repeatsAcknowledgmentsJohn Denton assisted with manuscript language revision.MetricsAuthor Information Riccardo Bartoletti Department of Urology, University of Florence, Florence, Italy More articles by this author Tommaso Cai Department of Urology, University of Florence, Florence, Italy More articles by this author Maurizio Dal Canto Department of Urology, University of Florence, Florence, Italy More articles by this author Vieri Boddi Department of Public Health and Epidemiology, University of Florence, Florence, Italy More articles by this author Gabriella Nesi Department of Pathology, University of Florence, Florence, Italy More articles by this author Mauro Piazzini Medical Genetics, University of Florence, Florence, Italy More articles by this author Expand All Advertisement PDF downloadLoading ..." @default.
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• W2126084722 title "Multiplex Polymerase Chain Reaction for Microsatellite Analysis of Urine Sediment Cells: A Rapid and Inexpensive Method for Diagnosing and Monitoring Superficial Transitional Bladder Cell Carcinoma" @default.
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