FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2126203831> ?p ?o ?g. }

• W2126203831 endingPage "5392" @default.
• W2126203831 startingPage "5384" @default.
• W2126203831 abstract "We mapped the in vivo phosphorylation sites for the matrix (M) protein of the Orsay and San Juan strains of vesicular stomatitis virus, Indiana serotype, using limited proteolysis and phosphoamino acid analysis. M protein was solubilized from 32P-labeled virions by using detergent and high-salt conditions, then treated with either trypsin or Staphylococcus aureus V8 protease, and analyzed by polyacrylamide gel electrophoresis and autoradiography to determine which fragments contained phosphate residues. The M protein fragment extending from amino acid 20 to the carboxy terminus contained approximately 70% of the control 32P label, while the fragment extending from amino acid 35 to the carboxy terminus had only trace amounts of label. These data indicate that the major phosphorylation site was between amino acids 20 and 34 in the Orsay strain M protein. Phosphoamino acid analysis of M protein by thin-layer electrophoresis showed the presence of phosphothreonine and phosphoserine and that phosphothreonine continued to be released after prolonged vapor-phase acid hydrolysis. These data identify Thr-31 as the primary in vivo phosphate acceptor for M protein of the Orsay strain of vesicular stomatitis virus. The San Juan strain M protein has serine at position 32, which may also be an important phosphate acceptor. In addition, phosphorylation at Ser-2, -3, or -17 occurs to a greater extent in the San Juan strain M protein than in the Orsay strain M protein. The subcellular distribution of phosphorylated M protein was investigated to determine a probable intracellular site(s) of phosphorylation. Phosphorylated M protein was associated primarily with cellular membranes, suggesting phosphorylation by a membrane-associated kinase. Virion M protein was phosphorylated to a greater extent than membrane-bound M protein, indicating that M protein phosphorylation occurs at a late stage in virus assembly. Phosphorylation of wild-type and temperature-sensitive mutant M protein was studied in vivo at the nonpermissive temperature. The data show that phosphorylated M protein was detected only in wild-type virus-infected cells and virions, suggesting that association with nucleocapsids may be required for M protein phosphorylation or that misfolding of mutant M protein at the nonpermissive temperature prevents phosphorylation." @default.
• W2126203831 created "2016-06-24" @default.
• W2126203831 creator A5042758683 @default.
• W2126203831 creator A5043678851 @default.
• W2126203831 creator A5067622298 @default.
• W2126203831 date "1992-09-01" @default.
• W2126203831 modified "2023-10-16" @default.
• W2126203831 title "Sites of in vivo phosphorylation of vesicular stomatitis virus matrix protein" @default.
• W2126203831 cites W1002836587 @default.
• W2126203831 cites W1499775870 @default.
• W2126203831 cites W1513827055 @default.
• W2126203831 cites W1532844378 @default.
• W2126203831 cites W1546363523 @default.
• W2126203831 cites W1555247430 @default.
• W2126203831 cites W1593142949 @default.
• W2126203831 cites W1596441602 @default.
• W2126203831 cites W1600904956 @default.
• W2126203831 cites W1616609814 @default.
• W2126203831 cites W1629956025 @default.
• W2126203831 cites W1772719730 @default.
• W2126203831 cites W1775749144 @default.
• W2126203831 cites W1884088573 @default.
• W2126203831 cites W1919175732 @default.
• W2126203831 cites W1920895567 @default.
• W2126203831 cites W1978002317 @default.
• W2126203831 cites W1984958007 @default.
• W2126203831 cites W1987103453 @default.
• W2126203831 cites W1988376512 @default.
• W2126203831 cites W2004917770 @default.
• W2126203831 cites W2007187604 @default.
• W2126203831 cites W2037375741 @default.
• W2126203831 cites W2045363450 @default.
• W2126203831 cites W2087847210 @default.
• W2126203831 cites W2088125974 @default.
• W2126203831 cites W2093840510 @default.
• W2126203831 cites W2105430517 @default.
• W2126203831 cites W2125266742 @default.
• W2126203831 cites W2155360072 @default.
• W2126203831 cites W981365008 @default.
• W2126203831 doi "https://doi.org/10.1128/jvi.66.9.5384-5392.1992" @default.
• W2126203831 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/289094" @default.
• W2126203831 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1323702" @default.
• W2126203831 hasPublicationYear "1992" @default.
• W2126203831 type Work @default.
• W2126203831 sameAs 2126203831 @default.
• W2126203831 citedByCount "11" @default.
• W2126203831 countsByYear W21262038312012 @default.
• W2126203831 countsByYear W21262038312018 @default.
• W2126203831 crossrefType "journal-article" @default.
• W2126203831 hasAuthorship W2126203831A5042758683 @default.
• W2126203831 hasAuthorship W2126203831A5043678851 @default.
• W2126203831 hasAuthorship W2126203831A5067622298 @default.
• W2126203831 hasBestOaLocation W21262038311 @default.
• W2126203831 hasConcept C104317684 @default.
• W2126203831 hasConcept C11960822 @default.
• W2126203831 hasConcept C153911025 @default.
• W2126203831 hasConcept C159047783 @default.
• W2126203831 hasConcept C167625842 @default.
• W2126203831 hasConcept C2522874641 @default.
• W2126203831 hasConcept C2776414213 @default.
• W2126203831 hasConcept C2779057330 @default.
• W2126203831 hasConcept C2780307904 @default.
• W2126203831 hasConcept C515207424 @default.
• W2126203831 hasConcept C55493867 @default.
• W2126203831 hasConcept C68434959 @default.
• W2126203831 hasConcept C86803240 @default.
• W2126203831 hasConcept C87325107 @default.
• W2126203831 hasConcept C97029542 @default.
• W2126203831 hasConceptScore W2126203831C104317684 @default.
• W2126203831 hasConceptScore W2126203831C11960822 @default.
• W2126203831 hasConceptScore W2126203831C153911025 @default.
• W2126203831 hasConceptScore W2126203831C159047783 @default.
• W2126203831 hasConceptScore W2126203831C167625842 @default.
• W2126203831 hasConceptScore W2126203831C2522874641 @default.
• W2126203831 hasConceptScore W2126203831C2776414213 @default.
• W2126203831 hasConceptScore W2126203831C2779057330 @default.
• W2126203831 hasConceptScore W2126203831C2780307904 @default.
• W2126203831 hasConceptScore W2126203831C515207424 @default.
• W2126203831 hasConceptScore W2126203831C55493867 @default.
• W2126203831 hasConceptScore W2126203831C68434959 @default.
• W2126203831 hasConceptScore W2126203831C86803240 @default.
• W2126203831 hasConceptScore W2126203831C87325107 @default.
• W2126203831 hasConceptScore W2126203831C97029542 @default.
• W2126203831 hasIssue "9" @default.
• W2126203831 hasLocation W21262038311 @default.
• W2126203831 hasLocation W21262038312 @default.
• W2126203831 hasLocation W21262038313 @default.
• W2126203831 hasLocation W21262038314 @default.
• W2126203831 hasOpenAccess W2126203831 @default.
• W2126203831 hasPrimaryLocation W21262038311 @default.
• W2126203831 hasRelatedWork W109096009 @default.
• W2126203831 hasRelatedWork W1844240163 @default.
• W2126203831 hasRelatedWork W1972191247 @default.
• W2126203831 hasRelatedWork W1973859009 @default.
• W2126203831 hasRelatedWork W2055289853 @default.
• W2126203831 hasRelatedWork W2071600423 @default.
• W2126203831 hasRelatedWork W2087945599 @default.
• W2126203831 hasRelatedWork W2606921482 @default.

• next