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• W2126216011 abstract "Though exogenous nerve growth factor (NGF) has been implicated in spinal cord plasticity, whether endogenous NGF plays a crucial role has not been established in vivo. This study investigated first the role of endogenous NGF in spinal dorsal horn (DH) plasticity following removal of L1-L5 and L7-S2 dorsal root ganglions (DRGs) in cats. Co-culture of chick embryo DRG with DH condition media, protein band fishing by cells as well as western blot showed that NGF could promote neurite growth in vitro. Immunohistochemistry and in situ hybridization technique revealed an increase in the NGF and NGF mRNA immunoreactive cells in the DH after partial deafferentation. Lastly, after blocking with NGF antibody, choleragen subunit B horseradish peroxidase (CB-HRP) tracing showed a reduction in the neuronal sprouting observed in the DH. Our results demonstrated that in the cat, endogenous NGF plays a crucial role in DH plasticity after partial deafferentation." @default.
• W2126216011 created "2016-06-24" @default.
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• W2126216011 date "2011-02-04" @default.
• W2126216011 modified "2023-10-10" @default.
• W2126216011 title "Crucial roles of NGF in dorsal horn plasticity in partially deafferentated cats" @default.
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• W2126216011 doi "https://doi.org/10.3109/08977194.2010.549129" @default.
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• W2126216011 hasPublicationYear "2011" @default.
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