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• W2126301092 abstract "Factors influencing apoptosis of vertebrate eggs and early embryos have been studied in cell-free systems and in intact embryos by analyzing individual apoptotic regulators or caspase activation in static samples. A novel method for monitoring caspase activity in living Xenopus oocytes and early embryos is described here. The approach, using microinjection of a near-infrared caspase substrate that emits fluorescence only after its proteolytic cleavage by active effector caspases, has enabled the elucidation of otherwise cryptic aspects of apoptotic regulation. In particular, we show that brief caspase activity (10 min) is sufficient to cause apoptotic death in this system. We illustrate a cytochrome c dose threshold in the oocyte, which is lowered by Smac, a protein that binds thereby neutralizing the inhibitor of apoptosis proteins. We show that meiotic oocytes develop resistance to cytochrome c, and that the eventual death of oocytes arrested in meiosis is caspase-independent. Finally, data acquired through imaging caspase activity in the Xenopus embryo suggest that apoptosis in very early development is not cell-autonomous. These studies both validate this assay as a useful tool for apoptosis research and reveal subtleties in the cell death program during early development. Moreover, this method offers a potentially valuable screening modality for identifying novel apoptotic regulators." @default.
• W2126301092 created "2016-06-24" @default.
• W2126301092 creator A5053532303 @default.
• W2126301092 creator A5061967975 @default.
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• W2126301092 date "2009-09-04" @default.
• W2126301092 modified "2023-10-14" @default.
• W2126301092 title "Features of programmed cell death in intact Xenopus oocytes and early embryos revealed by near-infrared fluorescence and real-time monitoring" @default.
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• W2126301092 doi "https://doi.org/10.1038/cdd.2009.120" @default.
• W2126301092 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2794955" @default.
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• W2126301092 hasPublicationYear "2009" @default.
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