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- W2126340602 abstract "In order to evaluate the functions of putative transcriptional regulatory regions of cotton genes, the upstream regulatory sequences (promoters) of two cotton genes, Gh-sp (seed protein) and Gh-rbcS (ribulose-1,5-bisphosphate carboxylase small subunit), were fused with a bb-glucuronidase (GUS) reporter gene and the cauliflower mosaic virus (CaMV) 35S terminator. These two gene constructs were transferred into cotton (Gossypium hirsutum L. cv. Coker 312) by Agrobacterium-mediated transformation. Transgenic plants from the T 0 generation were analyzed for expression of the GUS reporter gene in different tissues and developmental stages of cotton. Qualitative and quantitative analyses indicated the GUS gene driven by the Gh-sp promoter were expressed only during seed maturation, beginning approximately 25 d postanthesis. Expression of the GUS reporter gene driven by the Gh-rbcS promoter was detected primarily in leaf tissue from transgenic plants. Levels of GUS expression in leaves of Gh-rbcS/GUS transgenic plants was comparable to that in transgenic cotton plants containing a GUS gene construct controlled by the CaMV 35S promoter. These results indicate that these two tissue-specific promoters have the potential to be used to differentially express interesting genes in developing seeds or leaves of transgenic cotton." @default.
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- W2126340602 date "2000-01-01" @default.
- W2126340602 modified "2023-09-23" @default.
- W2126340602 title "Molecular Biology Expression of Two Tissue-Specific Promoters in Transgenic Cotton Plants" @default.
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