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• W2126342492 abstract "While cell-free systems are increasingly used for protein expression in structural and functional studies, several proteins are difficult to express or expressed only at low levels in cell-free lysates. Here, we report that fusion of the human immunoglobulin κ light chain constant domain (Cκ) at the C terminus of four representative proteins dramatically improved their production in the Escherichia coli S30 system, suggesting that enhancement of cell-free protein expression by Cκ fusion will be widely applicable." @default.
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• W2126342492 date "2006-12-01" @default.
• W2126342492 modified "2023-09-27" @default.
• W2126342492 title "Enhanced cell-free protein expression by fusion with immunoglobulin Cκ domain" @default.
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• W2126342492 doi "https://doi.org/10.1110/ps.062429906" @default.
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