FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W212634719> ?p ?o ?g. }

• W212634719 endingPage "861" @default.
• W212634719 startingPage "853" @default.
• W212634719 abstract "NAD+-dependent L-valine dehydrogenase was purified 180-fold from Streptomyces cinnamonensis, and to homogeneity, as judged by gel electrophoresis. The enzyme has an Mr of 88,000, and appears to be composed of subunits of Mr 41,200. The enzyme catalyses the oxidative deamination of L-valine, L-leucine, L-2-aminobutyric acid, L-norvaline and L-isoleucine, as well as the reductive amination of their 2-oxo analogues. The enzyme requires NAD+ as the only cofactor, which cannot be replaced by NADP+. The enzyme activity is significantly decreased by thiol-reactive reagents, although purine and pyrimidine bases, and nucleotides, do not affect activity. Initial-velocity and product-inhibition studies show that the reductive amination proceeds through a sequential ordered ternary-binary mechanism; NADH binds to the enzyme first, followed by 2-oxoisovalerate and NH3, and valine is released first, followed by NAD+. The Michaelis constants are as follows; L-valine, 1.3 mM; NAD+, 0.18 mM; NADH, 74 microM; 2-oxoisovalerate, 0.81 mM; and NH3, 55 mM. The pro-S hydrogen at C-4' of NADH is transferred to the substrate; the enzyme is B-stereospecific. It is proposed that the enzyme catalyses the first step of valine catabolism in this organism." @default.
• W212634719 created "2016-06-24" @default.
• W212634719 creator A5029640432 @default.
• W212634719 creator A5041549776 @default.
• W212634719 date "1989-08-01" @default.
• W212634719 modified "2023-10-13" @default.
• W212634719 title "Purification and catalytic properties of <scp>l</scp>-valine dehydrogenase from <i>Streptomyces cinnamonensis</i>" @default.
• W212634719 cites W1481914754 @default.
• W212634719 cites W1486899617 @default.
• W212634719 cites W1507088915 @default.
• W212634719 cites W1517356485 @default.
• W212634719 cites W1518238285 @default.
• W212634719 cites W1527159949 @default.
• W212634719 cites W1527204342 @default.
• W212634719 cites W1543819219 @default.
• W212634719 cites W1544878549 @default.
• W212634719 cites W1548124641 @default.
• W212634719 cites W1553335616 @default.
• W212634719 cites W1793869430 @default.
• W212634719 cites W1924867606 @default.
• W212634719 cites W1999954425 @default.
• W212634719 cites W2014321075 @default.
• W212634719 cites W2018289835 @default.
• W212634719 cites W2024019603 @default.
• W212634719 cites W2031963558 @default.
• W212634719 cites W2059574907 @default.
• W212634719 cites W2075118772 @default.
• W212634719 cites W2100837269 @default.
• W212634719 cites W2117338481 @default.
• W212634719 cites W2126944225 @default.
• W212634719 cites W2128635872 @default.
• W212634719 cites W2132011614 @default.
• W212634719 cites W2152957250 @default.
• W212634719 cites W2168293121 @default.
• W212634719 cites W2403788130 @default.
• W212634719 cites W2969273703 @default.
• W212634719 cites W39338687 @default.
• W212634719 doi "https://doi.org/10.1042/bj2610853" @default.
• W212634719 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1138909" @default.
• W212634719 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2803248" @default.
• W212634719 hasPublicationYear "1989" @default.
• W212634719 type Work @default.
• W212634719 sameAs 212634719 @default.
• W212634719 citedByCount "29" @default.
• W212634719 countsByYear W2126347192012 @default.
• W212634719 countsByYear W2126347192014 @default.
• W212634719 countsByYear W2126347192017 @default.
• W212634719 countsByYear W2126347192020 @default.
• W212634719 countsByYear W2126347192022 @default.
• W212634719 crossrefType "journal-article" @default.
• W212634719 hasAuthorship W212634719A5029640432 @default.
• W212634719 hasAuthorship W212634719A5041549776 @default.
• W212634719 hasBestOaLocation W2126347192 @default.
• W212634719 hasConcept C161790260 @default.
• W212634719 hasConcept C181199279 @default.
• W212634719 hasConcept C185592680 @default.
• W212634719 hasConcept C197957613 @default.
• W212634719 hasConcept C197974221 @default.
• W212634719 hasConcept C2776317432 @default.
• W212634719 hasConcept C2777573094 @default.
• W212634719 hasConcept C2779938440 @default.
• W212634719 hasConcept C515207424 @default.
• W212634719 hasConcept C55493867 @default.
• W212634719 hasConcept C71240020 @default.
• W212634719 hasConcept C75520062 @default.
• W212634719 hasConceptScore W212634719C161790260 @default.
• W212634719 hasConceptScore W212634719C181199279 @default.
• W212634719 hasConceptScore W212634719C185592680 @default.
• W212634719 hasConceptScore W212634719C197957613 @default.
• W212634719 hasConceptScore W212634719C197974221 @default.
• W212634719 hasConceptScore W212634719C2776317432 @default.
• W212634719 hasConceptScore W212634719C2777573094 @default.
• W212634719 hasConceptScore W212634719C2779938440 @default.
• W212634719 hasConceptScore W212634719C515207424 @default.
• W212634719 hasConceptScore W212634719C55493867 @default.
• W212634719 hasConceptScore W212634719C71240020 @default.
• W212634719 hasConceptScore W212634719C75520062 @default.
• W212634719 hasIssue "3" @default.
• W212634719 hasLocation W2126347191 @default.
• W212634719 hasLocation W2126347192 @default.
• W212634719 hasLocation W2126347193 @default.
• W212634719 hasLocation W2126347194 @default.
• W212634719 hasOpenAccess W212634719 @default.
• W212634719 hasPrimaryLocation W2126347191 @default.
• W212634719 hasRelatedWork W2003327451 @default.
• W212634719 hasRelatedWork W2037702679 @default.
• W212634719 hasRelatedWork W2049048597 @default.
• W212634719 hasRelatedWork W2080331064 @default.
• W212634719 hasRelatedWork W2081009067 @default.
• W212634719 hasRelatedWork W2103643682 @default.
• W212634719 hasRelatedWork W2405176976 @default.
• W212634719 hasRelatedWork W2429842276 @default.
• W212634719 hasRelatedWork W4206172903 @default.
• W212634719 hasRelatedWork W4290012698 @default.
• W212634719 hasVolume "261" @default.
• W212634719 isParatext "false" @default.
• W212634719 isRetracted "false" @default.
• W212634719 magId "212634719" @default.

• next