FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2126392252> ?p ?o ?g. }

• W2126392252 endingPage "1971" @default.
• W2126392252 startingPage "1962" @default.
• W2126392252 abstract "ABSTRACT A green fluorescent protein-based Pseudomonas fluorescens strain A506 biosensor was constructed and characterized for its potential to measure benzene, toluene, ethylbenzene, and related compounds in aqueous solutions. The biosensor is based on a plasmid carrying the toluene-benzene utilization ( tbu ) pathway transcriptional activator TbuT from Ralstonia pickettii PKO1 and a transcriptional fusion of its promoter PtbuA1 with a promoterless gfp gene on a broad-host-range promoter probe vector. TbuT was not limiting, since it was constitutively expressed by being fused to the neomycin phosphotransferase ( nptII ) promoter. The biosensor cells were readily induced, and fluorescence emission after induction periods of 3 h correlated well with toluene, benzene, ethylbenzene, and trichloroethylene concentrations. Our experiments using flow cytometry show that intermediate levels of gfp expression in response to toluene reflect uniform induction of cells. As the toluene concentration increases, the level of gfp expression per cell increases until saturation kinetics of the TbuT-PtbuA1 system are observed. Each inducer had a unique minimum concentration that was necessary for induction, with K app values that ranged from 3.3 ± 1.8 μM for toluene to 35.6 ± 16.6 μM for trichloroethylene (means ± standard errors of the means), and maximal fluorescence response. The fluorescence response was specific for alkyl-substituted benzene derivatives and branched alkenes (di- and trichloroethylene, 2-methyl-2-butene). The biosensor responded in an additive fashion to the presence of multiple inducers and was unaffected by the presence of compounds that were not inducers, such as those present in gasoline. Flow cytometry revealed that, in response to toxic concentrations of gasoline, there was a small uninduced population and another larger fully induced population whose levels of fluorescence corresponded to the amount of effectors present in the sample. These results demonstrate the potential for green fluorescent protein-based bacterial biosensors to measure environmental contaminants." @default.
• W2126392252 created "2016-06-24" @default.
• W2126392252 creator A5035405968 @default.
• W2126392252 creator A5082469862 @default.
• W2126392252 date "2002-04-01" @default.
• W2126392252 modified "2023-10-01" @default.
• W2126392252 title "Development and Characterization of a Green Fluorescent Protein-Based Bacterial Biosensor for Bioavailable Toluene and Related Compounds" @default.
• W2126392252 cites W1212215159 @default.
• W2126392252 cites W1530712533 @default.
• W2126392252 cites W1530879838 @default.
• W2126392252 cites W1610085996 @default.
• W2126392252 cites W1624099298 @default.
• W2126392252 cites W1649997932 @default.
• W2126392252 cites W1748737364 @default.
• W2126392252 cites W1794095044 @default.
• W2126392252 cites W1810581208 @default.
• W2126392252 cites W1836904192 @default.
• W2126392252 cites W1869278765 @default.
• W2126392252 cites W1972823382 @default.
• W2126392252 cites W1974690608 @default.
• W2126392252 cites W1992644222 @default.
• W2126392252 cites W2001196337 @default.
• W2126392252 cites W2009557363 @default.
• W2126392252 cites W2025160765 @default.
• W2126392252 cites W2036742051 @default.
• W2126392252 cites W2040986334 @default.
• W2126392252 cites W2049789822 @default.
• W2126392252 cites W2062221023 @default.
• W2126392252 cites W2070599701 @default.
• W2126392252 cites W2073403577 @default.
• W2126392252 cites W2105934377 @default.
• W2126392252 cites W2110844641 @default.
• W2126392252 cites W2113643142 @default.
• W2126392252 cites W2115563473 @default.
• W2126392252 cites W2127068865 @default.
• W2126392252 cites W2132922134 @default.
• W2126392252 cites W2136153667 @default.
• W2126392252 cites W2142896209 @default.
• W2126392252 cites W2155833324 @default.
• W2126392252 cites W2158937196 @default.
• W2126392252 cites W2160463209 @default.
• W2126392252 cites W4231749887 @default.
• W2126392252 cites W4254298129 @default.
• W2126392252 doi "https://doi.org/10.1128/aem.68.4.1962-1971.2002" @default.
• W2126392252 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/123894" @default.
• W2126392252 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11916719" @default.
• W2126392252 hasPublicationYear "2002" @default.
• W2126392252 type Work @default.
• W2126392252 sameAs 2126392252 @default.
• W2126392252 citedByCount "131" @default.
• W2126392252 countsByYear W21263922522012 @default.
• W2126392252 countsByYear W21263922522013 @default.
• W2126392252 countsByYear W21263922522014 @default.
• W2126392252 countsByYear W21263922522015 @default.
• W2126392252 countsByYear W21263922522016 @default.
• W2126392252 countsByYear W21263922522017 @default.
• W2126392252 countsByYear W21263922522018 @default.
• W2126392252 countsByYear W21263922522019 @default.
• W2126392252 countsByYear W21263922522020 @default.
• W2126392252 countsByYear W21263922522021 @default.
• W2126392252 countsByYear W21263922522022 @default.
• W2126392252 countsByYear W21263922522023 @default.
• W2126392252 crossrefType "journal-article" @default.
• W2126392252 hasAuthorship W2126392252A5035405968 @default.
• W2126392252 hasAuthorship W2126392252A5082469862 @default.
• W2126392252 hasBestOaLocation W21263922521 @default.
• W2126392252 hasConcept C104317684 @default.
• W2126392252 hasConcept C142613039 @default.
• W2126392252 hasConcept C153911025 @default.
• W2126392252 hasConcept C160756335 @default.
• W2126392252 hasConcept C178790620 @default.
• W2126392252 hasConcept C185592680 @default.
• W2126392252 hasConcept C2225953 @default.
• W2126392252 hasConcept C2777691172 @default.
• W2126392252 hasConcept C2778142098 @default.
• W2126392252 hasConcept C2779533485 @default.
• W2126392252 hasConcept C2780734198 @default.
• W2126392252 hasConcept C43617362 @default.
• W2126392252 hasConcept C553184892 @default.
• W2126392252 hasConcept C55493867 @default.
• W2126392252 hasConcept C86803240 @default.
• W2126392252 hasConceptScore W2126392252C104317684 @default.
• W2126392252 hasConceptScore W2126392252C142613039 @default.
• W2126392252 hasConceptScore W2126392252C153911025 @default.
• W2126392252 hasConceptScore W2126392252C160756335 @default.
• W2126392252 hasConceptScore W2126392252C178790620 @default.
• W2126392252 hasConceptScore W2126392252C185592680 @default.
• W2126392252 hasConceptScore W2126392252C2225953 @default.
• W2126392252 hasConceptScore W2126392252C2777691172 @default.
• W2126392252 hasConceptScore W2126392252C2778142098 @default.
• W2126392252 hasConceptScore W2126392252C2779533485 @default.
• W2126392252 hasConceptScore W2126392252C2780734198 @default.
• W2126392252 hasConceptScore W2126392252C43617362 @default.
• W2126392252 hasConceptScore W2126392252C553184892 @default.
• W2126392252 hasConceptScore W2126392252C55493867 @default.
• W2126392252 hasConceptScore W2126392252C86803240 @default.
• W2126392252 hasIssue "4" @default.
• W2126392252 hasLocation W21263922521 @default.

• next