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• W2126427023 endingPage "1505" @default.
• W2126427023 startingPage "1496" @default.
• W2126427023 abstract "Triticum mosaic virus (TriMV) (genus Poacevirus, family Potyviridae) is a recently described eriophyid mite-transmitted wheat virus. In vitro RNA transcripts generated from full-length cDNA clones of TriMV proved infectious on wheat. Wheat seedlings inoculated with in vitro transcripts elicited mosaic and mottling symptoms similar to the wild-type virus, and the progeny virus was efficiently transmitted by wheat curl mites, indicating that the cloned virus retained pathogenicity, movement, and wheat curl mite transmission characteristics. A series of TriMV-based expression vectors was constructed by engineering a green fluorescent protein (GFP) or red fluorescent protein (RFP) open reading frame with homologous NIa-Pro cleavage peptides between the P1 and HC-Pro cistrons. We found that GFP-tagged TriMV with seven or nine amino acid cleavage peptides efficiently processed GFP from HC-Pro. TriMV-GFP vectors were stable in wheat for more than 120 days and for six serial passages at 14-day intervals by mechanical inoculation and were transmitted by wheat curl mites similarly to the wild-type virus. Fluorescent protein-tagged TriMV was observed in wheat leaves, stems, and crowns. The availability of fluorescent protein-tagged TriMV will facilitate the examination of virus movement and distribution in cereal hosts and the mechanisms of cross protection and synergistic interactions between TriMV and Wheat streak mosaic virus." @default.
• W2126427023 created "2016-06-24" @default.
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• W2126427023 date "2015-11-01" @default.
• W2126427023 modified "2023-10-03" @default.
• W2126427023 title "In Vitro Transcripts of Wild-Type and Fluorescent Protein-Tagged <i>Triticum mosaic virus</i> (Family <i>Potyviridae</i>) are Biologically Active in Wheat" @default.
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• W2126427023 doi "https://doi.org/10.1094/phyto-06-15-0138-r" @default.
• W2126427023 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26214124" @default.
• W2126427023 hasPublicationYear "2015" @default.
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