FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2126649249> ?p ?o ?g. }

• W2126649249 abstract "ABSTRACT The bacterial RecA protein and its eukaryotic homologue Rad51 play a central role in the homologous DNA strand exchange reaction during recombination and DNA repair. Previously, our lab has shown that PfRad51, the Plasmodium falciparum homologue of Rad51, exhibited ATPase activity and promoted DNA strand exchange in vitro . In this study, we evaluated the catalytic functions of PfRad51 in the presence of putative interacting partners, especially P. falciparum homologues of Rad54 and replication protein A. PfRad54 accelerated PfRad51-mediated pairing between single-stranded DNA (ssDNA) and its homologous linear double-stranded DNA (dsDNA) in the presence of 0.5 mM CaCl 2 . We also present evidence that recombinant PfRPA1L protein serves the function of the bacterial homologue single-stranded binding protein (SSB) in initiating homologous pairing and strand exchange activity. More importantly, the function of PfRPA1L was negatively regulated in a dose-dependent manner by PfRPA1S, another RPA homologue in P. falciparum . Finally, we present in vivo evidence through comet assays for methyl methane sulfonate-induced DNA damage in malaria parasites and accompanying upregulation of PfRad51, PfRad54, PfRPA1L, and PfRPA1S at the level of transcript and protein needed to repair DNA damage. This study provides new insights into the role of putative Rad51-interacting proteins involved in homologous recombination and emphasizes the physiological role of DNA damage repair during the growth of parasites. IMPORTANCE Homologous recombination plays a major role in chromosomal rearrangement, and Rad51 protein, aided by several other proteins, plays a central role in DNA strand exchange reaction during recombination and DNA repair. This study reports on the characterization of the role of P. falciparum Rad51 in homologous strand exchange and DNA repair and evaluates the functional contribution of PfRad54 and PfRPA1 proteins. Data presented here provide mechanistic insights into DNA recombination and DNA damage repair mechanisms in this parasite. The importance of these research findings in future work will be to investigate if Rad51-dependent mechanisms are involved in chromosomal rearrangements during antigenic variation in P. falciparum . A prominent determinant of antigenic variation, the extraordinary ability of the parasite to rapidly change its surface molecules, is associated with var genes, and antigenic variation presents a major challenge to vaccine development." @default.
• W2126649249 created "2016-06-24" @default.
• W2126649249 creator A5054514931 @default.
• W2126649249 creator A5055673972 @default.
• W2126649249 date "2013-07-01" @default.
• W2126649249 modified "2023-10-03" @default.
• W2126649249 title "Opposing Roles for Two Molecular Forms of Replication Protein A in Rad51-Rad54-Mediated DNA Recombination in Plasmodium falciparum" @default.
• W2126649249 cites W1539685331 @default.
• W2126649249 cites W1566072364 @default.
• W2126649249 cites W1571689585 @default.
• W2126649249 cites W1676891985 @default.
• W2126649249 cites W1963831720 @default.
• W2126649249 cites W1966407135 @default.
• W2126649249 cites W1969503178 @default.
• W2126649249 cites W1975020577 @default.
• W2126649249 cites W1983134926 @default.
• W2126649249 cites W1990586926 @default.
• W2126649249 cites W1997091164 @default.
• W2126649249 cites W2006364890 @default.
• W2126649249 cites W2014199526 @default.
• W2126649249 cites W2016215265 @default.
• W2126649249 cites W2017229649 @default.
• W2126649249 cites W2022100127 @default.
• W2126649249 cites W2023722203 @default.
• W2126649249 cites W2026933272 @default.
• W2126649249 cites W2028084970 @default.
• W2126649249 cites W2032394001 @default.
• W2126649249 cites W2035746057 @default.
• W2126649249 cites W2042164753 @default.
• W2126649249 cites W2046062984 @default.
• W2126649249 cites W2057072455 @default.
• W2126649249 cites W2057673816 @default.
• W2126649249 cites W2061406721 @default.
• W2126649249 cites W2068887263 @default.
• W2126649249 cites W2080443362 @default.
• W2126649249 cites W2080977505 @default.
• W2126649249 cites W2083147879 @default.
• W2126649249 cites W2089804282 @default.
• W2126649249 cites W2092629964 @default.
• W2126649249 cites W2095554063 @default.
• W2126649249 cites W2108244474 @default.
• W2126649249 cites W2113147636 @default.
• W2126649249 cites W2122816558 @default.
• W2126649249 cites W2130669550 @default.
• W2126649249 cites W2139977478 @default.
• W2126649249 cites W2144840612 @default.
• W2126649249 cites W2154317163 @default.
• W2126649249 cites W2168166289 @default.
• W2126649249 cites W2169741450 @default.
• W2126649249 cites W4241219595 @default.
• W2126649249 cites W4246651551 @default.
• W2126649249 cites W4321429217 @default.
• W2126649249 doi "https://doi.org/10.1128/mbio.00252-13" @default.
• W2126649249 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3648904" @default.
• W2126649249 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23631919" @default.
• W2126649249 hasPublicationYear "2013" @default.
• W2126649249 type Work @default.
• W2126649249 sameAs 2126649249 @default.
• W2126649249 citedByCount "27" @default.
• W2126649249 countsByYear W21266492492013 @default.
• W2126649249 countsByYear W21266492492014 @default.
• W2126649249 countsByYear W21266492492015 @default.
• W2126649249 countsByYear W21266492492016 @default.
• W2126649249 countsByYear W21266492492017 @default.
• W2126649249 countsByYear W21266492492018 @default.
• W2126649249 countsByYear W21266492492019 @default.
• W2126649249 countsByYear W21266492492020 @default.
• W2126649249 countsByYear W21266492492021 @default.
• W2126649249 countsByYear W21266492492022 @default.
• W2126649249 crossrefType "journal-article" @default.
• W2126649249 hasAuthorship W2126649249A5054514931 @default.
• W2126649249 hasAuthorship W2126649249A5055673972 @default.
• W2126649249 hasBestOaLocation W21266492491 @default.
• W2126649249 hasConcept C101434241 @default.
• W2126649249 hasConcept C102744134 @default.
• W2126649249 hasConcept C104317684 @default.
• W2126649249 hasConcept C113271649 @default.
• W2126649249 hasConcept C134935766 @default.
• W2126649249 hasConcept C135300049 @default.
• W2126649249 hasConcept C143425029 @default.
• W2126649249 hasConcept C153911025 @default.
• W2126649249 hasConcept C156695909 @default.
• W2126649249 hasConcept C187882448 @default.
• W2126649249 hasConcept C19924922 @default.
• W2126649249 hasConcept C203284983 @default.
• W2126649249 hasConcept C2779012473 @default.
• W2126649249 hasConcept C40767141 @default.
• W2126649249 hasConcept C54355233 @default.
• W2126649249 hasConcept C552990157 @default.
• W2126649249 hasConcept C73573662 @default.
• W2126649249 hasConcept C86339819 @default.
• W2126649249 hasConcept C86803240 @default.
• W2126649249 hasConcept C94966510 @default.
• W2126649249 hasConcept C95444343 @default.
• W2126649249 hasConceptScore W2126649249C101434241 @default.
• W2126649249 hasConceptScore W2126649249C102744134 @default.
• W2126649249 hasConceptScore W2126649249C104317684 @default.
• W2126649249 hasConceptScore W2126649249C113271649 @default.
• W2126649249 hasConceptScore W2126649249C134935766 @default.
• W2126649249 hasConceptScore W2126649249C135300049 @default.

• next