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• W2126985107 abstract "ABSTRACT Shiga-toxigenic Escherichia coli (STEC) O113:H21 strains that lack the locus of enterocyte effacement (LEE) efficiently invade eukaryotic cells in vitro , unlike LEE-positive O157:H7 strains. We used a fliC deletion mutant of the O113:H21 STEC strain 98NK2 (98NK2Δ fliC ) to show that invasion of colonic epithelial (HCT-8) cells is heavily dependent on production of flagellin, even though adherence to the cells was actually enhanced in the mutant. Flagellin binds and signals through Toll-like receptor 5 (TLR5), but there was no evidence that either TLR5, the adaptor protein myeloid differentiation primary response gene 88 (MyD88), or the serine kinase interleukin-1 receptor-associated kinase (IRAK) were required for invasion of HCT-8 cells by strain 98NK2, as judged by transfection, RNA knockdown, or inhibitor studies. However, pretreatment of cells with anti-asialo-GM1 significantly decreased 98NK2 invasion (by 40.8%), while neuraminidase treatment (which cleaves terminal sialic acid residues, thus converting GM1 into asialo-GM1) significantly increased invasion (by 70.7%). Pretreatment of HCT-8 cells with either the cholesterol-depleting agent methyl-β-cyclodextrin (MβCD) or the tyrosine kinase inhibitor genistein significantly decreased invasion by 98NK2, indicating a potential role for lipid rafts in the invasion mechanism. Confocal microscopy also showed invading 98NK2 colocalized with lipid raft markers caveolin-1 and GM1. Interestingly, anti-asialo-GM1, neuraminidase, MβCD, and genistein have similar effects on the vestigial level of STEC invasion seen for STEC strain 98NK2Δ fliC , indicating that lipid rafts mediate a common step in flagellin-dependent and flagellin-independent cellular invasion." @default.
• W2126985107 created "2016-06-24" @default.
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• W2126985107 date "2012-08-01" @default.
• W2126985107 modified "2023-10-16" @default.
• W2126985107 title "Role of Lipid Rafts and Flagellin in Invasion of Colonic Epithelial Cells by Shiga-Toxigenic Escherichia coli O113:H21" @default.
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• W2126985107 doi "https://doi.org/10.1128/iai.00336-12" @default.
• W2126985107 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3434589" @default.
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