FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2126996947> ?p ?o ?g. }

• W2126996947 endingPage "479" @default.
• W2126996947 startingPage "468" @default.
• W2126996947 abstract "Efficient protein synthesis in bacteria requires initiation factor 2 (IF2), elongation factors Tu (EF-Tu) and G (EF-G), and release factor 3 (RF3), each of which catalyzes a major step of translation in a GTP-dependent fashion. Previous reports have suggested that recruitment of factors to the ribosome and subsequent GTP hydrolysis involve the dimeric protein L12, which forms a flexible stalk on the ribosome. Using heteronuclear NMR spectroscopy we demonstrate that L12 binds directly to the factors IF2, EF-Tu, EF-G, and RF3 from Escherichia coli, and map the region of L12 involved in these interactions. Factor-dependent chemical shift changes show that all four factors bind to the same region of the C-terminal domain of L12. This region includes three strictly conserved residues, K70, L80, and E82, and a set of highly conserved residues, including V66, A67, V68 and G79. Upon factor binding, all NMR signals from the C-terminal domain become broadened beyond detection, while those from the N-terminal domain are virtually unaffected, implying that the C-terminal domain binds to the factor, while the N-terminal domain dimer retains its rotational freedom mediated by the flexible hinge between the two domains. Factor-dependent variations in linewidths further reveal that L12 binds to each factor with a dissociation constant in the millimolar range in solution. These results indicate that the L12-factor complexes will be highly populated on the ribosome, because of the high local concentration of ribosome-bound factor with respect to L12." @default.
• W2126996947 created "2016-06-24" @default.
• W2126996947 creator A5033757533 @default.
• W2126996947 creator A5041024225 @default.
• W2126996947 creator A5054484167 @default.
• W2126996947 creator A5063659458 @default.
• W2126996947 creator A5081141506 @default.
• W2126996947 creator A5088683254 @default.
• W2126996947 date "2007-01-01" @default.
• W2126996947 modified "2023-09-24" @default.
• W2126996947 title "The Ribosomal Stalk Binds to Translation Factors IF2, EF-Tu, EF-G and RF3 via a Conserved Region of the L12 C-terminal Domain" @default.
• W2126996947 cites W1539889956 @default.
• W2126996947 cites W1605208286 @default.
• W2126996947 cites W1607300871 @default.
• W2126996947 cites W1607795610 @default.
• W2126996947 cites W1965257507 @default.
• W2126996947 cites W1966651071 @default.
• W2126996947 cites W1966849099 @default.
• W2126996947 cites W1970827957 @default.
• W2126996947 cites W1975992580 @default.
• W2126996947 cites W1977052971 @default.
• W2126996947 cites W1977189709 @default.
• W2126996947 cites W1983527475 @default.
• W2126996947 cites W1983534198 @default.
• W2126996947 cites W1994924626 @default.
• W2126996947 cites W1999652589 @default.
• W2126996947 cites W2002195659 @default.
• W2126996947 cites W2009810332 @default.
• W2126996947 cites W2018988920 @default.
• W2126996947 cites W2020346411 @default.
• W2126996947 cites W2023864622 @default.
• W2126996947 cites W2026042608 @default.
• W2126996947 cites W2029119597 @default.
• W2126996947 cites W2034304629 @default.
• W2126996947 cites W2034984854 @default.
• W2126996947 cites W2048646541 @default.
• W2126996947 cites W2049292673 @default.
• W2126996947 cites W2054511300 @default.
• W2126996947 cites W2058371301 @default.
• W2126996947 cites W2068154138 @default.
• W2126996947 cites W2069417649 @default.
• W2126996947 cites W2073149987 @default.
• W2126996947 cites W2074324186 @default.
• W2126996947 cites W2081540493 @default.
• W2126996947 cites W2082583578 @default.
• W2126996947 cites W2083490941 @default.
• W2126996947 cites W2087679230 @default.
• W2126996947 cites W2087905945 @default.
• W2126996947 cites W2092617311 @default.
• W2126996947 cites W2093471887 @default.
• W2126996947 cites W2095011159 @default.
• W2126996947 cites W2096817012 @default.
• W2126996947 cites W2119966574 @default.
• W2126996947 cites W2122866176 @default.
• W2126996947 cites W2129096064 @default.
• W2126996947 cites W2135985251 @default.
• W2126996947 cites W2137081760 @default.
• W2126996947 cites W2137206793 @default.
• W2126996947 cites W2150417688 @default.
• W2126996947 cites W2152348402 @default.
• W2126996947 cites W2159758084 @default.
• W2126996947 cites W2169707043 @default.
• W2126996947 cites W2169821755 @default.
• W2126996947 doi "https://doi.org/10.1016/j.jmb.2006.10.025" @default.
• W2126996947 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17070545" @default.
• W2126996947 hasPublicationYear "2007" @default.
• W2126996947 type Work @default.
• W2126996947 sameAs 2126996947 @default.
• W2126996947 citedByCount "117" @default.
• W2126996947 countsByYear W21269969472012 @default.
• W2126996947 countsByYear W21269969472013 @default.
• W2126996947 countsByYear W21269969472014 @default.
• W2126996947 countsByYear W21269969472015 @default.
• W2126996947 countsByYear W21269969472016 @default.
• W2126996947 countsByYear W21269969472017 @default.
• W2126996947 countsByYear W21269969472018 @default.
• W2126996947 countsByYear W21269969472019 @default.
• W2126996947 countsByYear W21269969472020 @default.
• W2126996947 countsByYear W21269969472021 @default.
• W2126996947 countsByYear W21269969472022 @default.
• W2126996947 countsByYear W21269969472023 @default.
• W2126996947 crossrefType "journal-article" @default.
• W2126996947 hasAuthorship W2126996947A5033757533 @default.
• W2126996947 hasAuthorship W2126996947A5041024225 @default.
• W2126996947 hasAuthorship W2126996947A5054484167 @default.
• W2126996947 hasAuthorship W2126996947A5063659458 @default.
• W2126996947 hasAuthorship W2126996947A5081141506 @default.
• W2126996947 hasAuthorship W2126996947A5088683254 @default.
• W2126996947 hasConcept C104317684 @default.
• W2126996947 hasConcept C12554922 @default.
• W2126996947 hasConcept C139124968 @default.
• W2126996947 hasConcept C178790620 @default.
• W2126996947 hasConcept C185592680 @default.
• W2126996947 hasConcept C2779546866 @default.
• W2126996947 hasConcept C38062823 @default.
• W2126996947 hasConcept C515207424 @default.
• W2126996947 hasConcept C55493867 @default.
• W2126996947 hasConcept C57711820 @default.

• next