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- W2127076278 abstract "[URE3] is a prion (infectious protein) of the Ure2 protein of yeast. In vitro, Ure2p can form amyloid filaments, but direct evidence that these filaments constitute the infectious form is still missing. Here we demonstrate that recombinant Ure2p converted into amyloid can infect yeast cells lacking the prion. Infection produced a variety of [URE3] variants. Extracts of [URE3] strains, as well as amyloid of Ure2p formed in an extract-primed reaction could transmit to uninfected cells the [URE3] variant present in the cells from which the extracts were made. Infectivity and determinant of [URE3] variants resided within the N-terminal 65 amino acids of Ure2p. Notably, we could show that amyloid filaments of recombinant Ure2p are nearly as infectious per mass of Ure2p as extracts of [URE3] strains. Sizing experiments indicated that infectious particles in vitro and in vivo were >20 nm in diameter, suggesting that they were amyloid filaments and not smaller oligomeric structures. Our data indicate that there is no substantial difference between filaments formed in vivo and in vitro." @default.
- W2127076278 created "2016-06-24" @default.
- W2127076278 creator A5005541485 @default.
- W2127076278 creator A5022745225 @default.
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- W2127076278 date "2005-08-11" @default.
- W2127076278 modified "2023-09-30" @default.
- W2127076278 title "Prion generation in vitro: amyloid of Ure2p is infectious" @default.
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- W2127076278 doi "https://doi.org/10.1038/sj.emboj.7600772" @default.
- W2127076278 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1201353" @default.
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