Matches in SemOpenAlex for { <https://semopenalex.org/work/W2127233764> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2127233764 abstract "The early diagnosis of Alzheimer's disease (AD) is critical for the evaluation of new therapies and for patient follow-up. Aggregation of Aβ 1-40, Aβ 1-42 peptides in amyloid plaques, one of the major hallmarks of the pathology, begins more than 15years before neuropsychological symptoms. Few methods have been developed to detect amyloid plaques by MRI but they always required an invasive process to open the blood brain barrier (BBB). Heavy chain only antibody fragments (VHH), because of their small size and their basic isoelectric point, have the potential to cross the BBB. Hence, in order to detect amyloid plaques by MRI, we developed VHHs with high specificity against Aβ 1-40, Aβ 1-42. First, we evaluated the BBB crossing by in vivo multi-photon imaging after conjugation of our VHHs to a fluorochrome. Then, gadolinium (Gd) was conjugated to VHHs via a DOTA cage and the resulting contrast agent (VHH-DOTA/Gd) was evaluated by MRI. Conjugates were synthesized by conjugation of Alexa-Fluor488 or a DOTA/Gd synthon on VHHs. Conjugates were monitored by HPLC/MS, and binding properties were confirmed by ELISA and IHC. PS2APP mice aged of 90-100weeks were used (n=2/experiment). In vivo multi-photon experiments were realized after intravenous injections of VHH-AlexaFluor488. MRI acquisition were recorded following two different protocols 1) in vitro incubation of one hemisphere with VHH-DOTA/Gd (0.1mg/ml equivalent to 0.1mM of Gd) overnight, 2) Gd-staining, a reference technique to reveal amyloid plaques by soaking the hemisphere in a Gd-solution (2.5mM - 48h). Live multi-photon imaging following intravenous injection showed gradual extravasation of the fluorescent VHHs from blood vessels and penetration in brain parenchyma with an exquisite tropism for amyloid plaques, as confirmed by IHC (Figure 1). MR images obtained after in vitro incubation with VHH-DOTA/Gd showed numerous hypointense spots in the cortex (Figure 2A). Moreover, several hypointense spots were colocalised (red arrows) with amyloid plaques revealed by the reference technique of Gd-staining (Figure 2B)." @default.
- W2127233764 created "2016-06-24" @default.
- W2127233764 creator A5003701057 @default.
- W2127233764 creator A5004498679 @default.
- W2127233764 creator A5004984882 @default.
- W2127233764 creator A5019078890 @default.
- W2127233764 creator A5025330933 @default.
- W2127233764 creator A5038877213 @default.
- W2127233764 creator A5039383646 @default.
- W2127233764 creator A5050550539 @default.
- W2127233764 creator A5057540913 @default.
- W2127233764 creator A5063954532 @default.
- W2127233764 creator A5065043368 @default.
- W2127233764 creator A5082644366 @default.
- W2127233764 date "2014-07-01" @default.
- W2127233764 modified "2023-09-25" @default.
- W2127233764 title "IC-P-015: NEW TOOLS FOR AMYLOID PLAQUES DETECTION BY MRI: GADOLINIUM-VHH ANTIBODY CONJUGATES" @default.
- W2127233764 doi "https://doi.org/10.1016/j.jalz.2014.05.019" @default.
- W2127233764 hasPublicationYear "2014" @default.
- W2127233764 type Work @default.
- W2127233764 sameAs 2127233764 @default.
- W2127233764 citedByCount "0" @default.
- W2127233764 crossrefType "journal-article" @default.
- W2127233764 hasAuthorship W2127233764A5003701057 @default.
- W2127233764 hasAuthorship W2127233764A5004498679 @default.
- W2127233764 hasAuthorship W2127233764A5004984882 @default.
- W2127233764 hasAuthorship W2127233764A5019078890 @default.
- W2127233764 hasAuthorship W2127233764A5025330933 @default.
- W2127233764 hasAuthorship W2127233764A5038877213 @default.
- W2127233764 hasAuthorship W2127233764A5039383646 @default.
- W2127233764 hasAuthorship W2127233764A5050550539 @default.
- W2127233764 hasAuthorship W2127233764A5057540913 @default.
- W2127233764 hasAuthorship W2127233764A5063954532 @default.
- W2127233764 hasAuthorship W2127233764A5065043368 @default.
- W2127233764 hasAuthorship W2127233764A5082644366 @default.
- W2127233764 hasConcept C134306372 @default.
- W2127233764 hasConcept C142724271 @default.
- W2127233764 hasConcept C150903083 @default.
- W2127233764 hasConcept C153911025 @default.
- W2127233764 hasConcept C159654299 @default.
- W2127233764 hasConcept C178790620 @default.
- W2127233764 hasConcept C185592680 @default.
- W2127233764 hasConcept C197336794 @default.
- W2127233764 hasConcept C203014093 @default.
- W2127233764 hasConcept C207001950 @default.
- W2127233764 hasConcept C2777633098 @default.
- W2127233764 hasConcept C2777820669 @default.
- W2127233764 hasConcept C2779931791 @default.
- W2127233764 hasConcept C33923547 @default.
- W2127233764 hasConcept C557651011 @default.
- W2127233764 hasConcept C71924100 @default.
- W2127233764 hasConcept C86803240 @default.
- W2127233764 hasConceptScore W2127233764C134306372 @default.
- W2127233764 hasConceptScore W2127233764C142724271 @default.
- W2127233764 hasConceptScore W2127233764C150903083 @default.
- W2127233764 hasConceptScore W2127233764C153911025 @default.
- W2127233764 hasConceptScore W2127233764C159654299 @default.
- W2127233764 hasConceptScore W2127233764C178790620 @default.
- W2127233764 hasConceptScore W2127233764C185592680 @default.
- W2127233764 hasConceptScore W2127233764C197336794 @default.
- W2127233764 hasConceptScore W2127233764C203014093 @default.
- W2127233764 hasConceptScore W2127233764C207001950 @default.
- W2127233764 hasConceptScore W2127233764C2777633098 @default.
- W2127233764 hasConceptScore W2127233764C2777820669 @default.
- W2127233764 hasConceptScore W2127233764C2779931791 @default.
- W2127233764 hasConceptScore W2127233764C33923547 @default.
- W2127233764 hasConceptScore W2127233764C557651011 @default.
- W2127233764 hasConceptScore W2127233764C71924100 @default.
- W2127233764 hasConceptScore W2127233764C86803240 @default.
- W2127233764 hasIssue "4S_Part_5" @default.
- W2127233764 hasLocation W21272337641 @default.
- W2127233764 hasOpenAccess W2127233764 @default.
- W2127233764 hasPrimaryLocation W21272337641 @default.
- W2127233764 hasRelatedWork W1973804528 @default.
- W2127233764 hasRelatedWork W2003466282 @default.
- W2127233764 hasRelatedWork W2026093192 @default.
- W2127233764 hasRelatedWork W2026186063 @default.
- W2127233764 hasRelatedWork W2044676806 @default.
- W2127233764 hasRelatedWork W2066444468 @default.
- W2127233764 hasRelatedWork W2162597084 @default.
- W2127233764 hasRelatedWork W2343033335 @default.
- W2127233764 hasRelatedWork W2899084033 @default.
- W2127233764 hasRelatedWork W3116448221 @default.
- W2127233764 hasVolume "10" @default.
- W2127233764 isParatext "false" @default.
- W2127233764 isRetracted "false" @default.
- W2127233764 magId "2127233764" @default.
- W2127233764 workType "article" @default.