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- W2127235556 endingPage "1035" @default.
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- W2127235556 abstract "Background: Hyaluronic acid (HA) is the most abundant glycosaminoglycan of vertebrate extracellular spaces and is specifically degraded by a β-1,4 glycosidase. Bee venom hyaluronidase (Hya) shares 30% sequence identity with human hyaluronidases, which are involved in fertilization and the turnover of HA. On the basis of sequence similarity, mammalian enzymes and Hya are assigned to glycosidase family 56 for which no structure has been reported yet.Results: The crystal structure of recombinant (Baculovirus) Hya was determined at 1.6 Å resolution. The overall topology resembles a classical (β/α)8 TIM barrel except that the barrel is composed of only seven strands. A long substrate binding groove extends across the C-terminal end of the barrel. Cocrystallization with a substrate analog revealed the presence of a HA tetramer bound to subsites −4 to −1 and distortion of the −1 sugar.Conclusions: The structure of the complex strongly suggest an acid–base catalytic mechanism, in which Glu113 acts as the proton donor and the N-acetyl group of the substrate is the nucleophile. The location of the catalytic residues shows striking similarity to bacterial chitinase which also operates via a substrate-assisted mechanism." @default.
- W2127235556 created "2016-06-24" @default.
- W2127235556 creator A5002943998 @default.
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- W2127235556 creator A5069251556 @default.
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- W2127235556 date "2000-10-01" @default.
- W2127235556 modified "2023-10-12" @default.
- W2127235556 title "Crystal Structure of Hyaluronidase, a Major Allergen of Bee Venom" @default.
- W2127235556 cites W1554121416 @default.
- W2127235556 cites W1591680814 @default.
- W2127235556 cites W1830745998 @default.
- W2127235556 cites W1935712031 @default.
- W2127235556 cites W1968938754 @default.
- W2127235556 cites W1969237045 @default.
- W2127235556 cites W1971970683 @default.
- W2127235556 cites W1972367491 @default.
- W2127235556 cites W1974056949 @default.
- W2127235556 cites W1986191025 @default.
- W2127235556 cites W1987250042 @default.
- W2127235556 cites W1990762118 @default.
- W2127235556 cites W1992774855 @default.
- W2127235556 cites W1993404146 @default.
- W2127235556 cites W1994528810 @default.
- W2127235556 cites W1995017064 @default.
- W2127235556 cites W1995277973 @default.
- W2127235556 cites W2001641653 @default.
- W2127235556 cites W2004584911 @default.
- W2127235556 cites W2005433675 @default.
- W2127235556 cites W2007829111 @default.
- W2127235556 cites W2008469454 @default.
- W2127235556 cites W2012856015 @default.
- W2127235556 cites W2013083986 @default.
- W2127235556 cites W2015478270 @default.
- W2127235556 cites W2018456240 @default.
- W2127235556 cites W2020487872 @default.
- W2127235556 cites W2023037761 @default.
- W2127235556 cites W2023425732 @default.
- W2127235556 cites W2023602101 @default.
- W2127235556 cites W2027752086 @default.
- W2127235556 cites W2028533348 @default.
- W2127235556 cites W2030337803 @default.
- W2127235556 cites W2033071347 @default.
- W2127235556 cites W2034660356 @default.
- W2127235556 cites W2040628201 @default.
- W2127235556 cites W2044868973 @default.
- W2127235556 cites W2045014705 @default.
- W2127235556 cites W2051921179 @default.
- W2127235556 cites W2056186597 @default.
- W2127235556 cites W2059737849 @default.
- W2127235556 cites W2060183170 @default.
- W2127235556 cites W2061900269 @default.
- W2127235556 cites W2065893364 @default.
- W2127235556 cites W2066243047 @default.
- W2127235556 cites W2067887310 @default.
- W2127235556 cites W2068265376 @default.
- W2127235556 cites W2072144575 @default.
- W2127235556 cites W2073412555 @default.
- W2127235556 cites W2080476827 @default.
- W2127235556 cites W2095237995 @default.
- W2127235556 cites W2101446086 @default.
- W2127235556 cites W2105738898 @default.
- W2127235556 cites W2106121486 @default.
- W2127235556 cites W2106325630 @default.
- W2127235556 cites W2112185869 @default.
- W2127235556 cites W2113530505 @default.
- W2127235556 cites W2113964973 @default.
- W2127235556 cites W2115339056 @default.
- W2127235556 cites W2122932778 @default.
- W2127235556 cites W2151900576 @default.
- W2127235556 cites W2155628954 @default.
- W2127235556 cites W2160181867 @default.
- W2127235556 cites W2166048948 @default.
- W2127235556 cites W2170264257 @default.
- W2127235556 cites W4235079696 @default.
- W2127235556 cites W4243231451 @default.
- W2127235556 doi "https://doi.org/10.1016/s0969-2126(00)00511-6" @default.
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