Matches in SemOpenAlex for { <https://semopenalex.org/work/W2127248031> ?p ?o ?g. }
- W2127248031 endingPage "e52966" @default.
- W2127248031 startingPage "e52966" @default.
- W2127248031 abstract "To obtain an overall picture of the repair of DNA single and double strand breaks in a defined region of chromatin in vivo, we studied their repair in a ∼170 kb circular minichromosome whose length and topology are analogous to those of the closed loops in genomic chromatin. The rate of repair of single strand breaks in cells irradiated with γ photons was quantitated by determining the sensitivity of the minichromosome DNA to nuclease S1, and that of double strand breaks by assaying the reformation of supercoiled DNA using pulsed field electrophoresis. Reformation of supercoiled DNA, which requires that all single strand breaks have been repaired, was not slowed detectably by the inhibitors of poly(ADP-ribose) polymerase-1 NU1025 or 1,5-IQD. Repair of double strand breaks was slowed by 20–30% when homologous recombination was supressed by KU55933, caffeine, or siRNA-mediated depletion of Rad51 but was completely arrested by the inhibitors of nonhomologous end-joining wortmannin or NU7441, responses interpreted as reflecting competition between these repair pathways similar to that seen in genomic DNA. The reformation of supercoiled DNA was unaffected when topoisomerases I or II, whose participation in repair of strand breaks has been controversial, were inhibited by the catalytic inhibitors ICRF-193 or F11782. Modeling of the kinetics of repair provided rate constants and showed that repair of single strand breaks in minichromosome DNA proceeded independently of repair of double strand breaks. The simplicity of quantitating strand breaks in this minichromosome provides a usefull system for testing the efficiency of new inhibitors of their repair, and since the sequence and structural features of its DNA and its transcription pattern have been studied extensively it offers a good model for examining other aspects of DNA breakage and repair." @default.
- W2127248031 created "2016-06-24" @default.
- W2127248031 creator A5039442781 @default.
- W2127248031 creator A5041406070 @default.
- W2127248031 creator A5048268944 @default.
- W2127248031 creator A5090127426 @default.
- W2127248031 creator A5091610453 @default.
- W2127248031 date "2013-01-30" @default.
- W2127248031 modified "2023-10-16" @default.
- W2127248031 title "Repair of DNA Strand Breaks in a Minichromosome In Vivo: Kinetics, Modeling, and Effects of Inhibitors" @default.
- W2127248031 cites W1497011606 @default.
- W2127248031 cites W1504511808 @default.
- W2127248031 cites W1505536714 @default.
- W2127248031 cites W1517857558 @default.
- W2127248031 cites W1584549675 @default.
- W2127248031 cites W1592483093 @default.
- W2127248031 cites W164322137 @default.
- W2127248031 cites W1801738628 @default.
- W2127248031 cites W1966051132 @default.
- W2127248031 cites W1966599755 @default.
- W2127248031 cites W1970107317 @default.
- W2127248031 cites W1970225082 @default.
- W2127248031 cites W1971563221 @default.
- W2127248031 cites W1972222169 @default.
- W2127248031 cites W1973023456 @default.
- W2127248031 cites W1975025621 @default.
- W2127248031 cites W1976475623 @default.
- W2127248031 cites W1979532417 @default.
- W2127248031 cites W1982916566 @default.
- W2127248031 cites W1983863777 @default.
- W2127248031 cites W1984889638 @default.
- W2127248031 cites W1986273700 @default.
- W2127248031 cites W1998836233 @default.
- W2127248031 cites W2004553600 @default.
- W2127248031 cites W2004835054 @default.
- W2127248031 cites W2006858302 @default.
- W2127248031 cites W2007369689 @default.
- W2127248031 cites W2010594589 @default.
- W2127248031 cites W2011720663 @default.
- W2127248031 cites W2014259806 @default.
- W2127248031 cites W2014334302 @default.
- W2127248031 cites W2016860667 @default.
- W2127248031 cites W2017742066 @default.
- W2127248031 cites W2019072372 @default.
- W2127248031 cites W2023581362 @default.
- W2127248031 cites W2025899010 @default.
- W2127248031 cites W2027379868 @default.
- W2127248031 cites W2028287260 @default.
- W2127248031 cites W2033176523 @default.
- W2127248031 cites W2033668903 @default.
- W2127248031 cites W2033709687 @default.
- W2127248031 cites W2034730235 @default.
- W2127248031 cites W2039477833 @default.
- W2127248031 cites W2040840466 @default.
- W2127248031 cites W2043889898 @default.
- W2127248031 cites W2045466228 @default.
- W2127248031 cites W2046833562 @default.
- W2127248031 cites W2053549853 @default.
- W2127248031 cites W2061527580 @default.
- W2127248031 cites W2064151299 @default.
- W2127248031 cites W2073687761 @default.
- W2127248031 cites W2076591557 @default.
- W2127248031 cites W2079853060 @default.
- W2127248031 cites W2081383642 @default.
- W2127248031 cites W2081702062 @default.
- W2127248031 cites W2082125616 @default.
- W2127248031 cites W2091884040 @default.
- W2127248031 cites W2092503176 @default.
- W2127248031 cites W2092793423 @default.
- W2127248031 cites W2097573505 @default.
- W2127248031 cites W2098581690 @default.
- W2127248031 cites W2101988823 @default.
- W2127248031 cites W2103328294 @default.
- W2127248031 cites W2103825714 @default.
- W2127248031 cites W2113526912 @default.
- W2127248031 cites W2116804853 @default.
- W2127248031 cites W2118211359 @default.
- W2127248031 cites W2119346494 @default.
- W2127248031 cites W2120922349 @default.
- W2127248031 cites W2121103789 @default.
- W2127248031 cites W2121672398 @default.
- W2127248031 cites W2123813971 @default.
- W2127248031 cites W2130532443 @default.
- W2127248031 cites W2131978108 @default.
- W2127248031 cites W2139229198 @default.
- W2127248031 cites W2142729720 @default.
- W2127248031 cites W2148961549 @default.
- W2127248031 cites W2158819412 @default.
- W2127248031 cites W2161489869 @default.
- W2127248031 cites W2161560609 @default.
- W2127248031 cites W2163938614 @default.
- W2127248031 cites W2164170763 @default.
- W2127248031 cites W2164995160 @default.
- W2127248031 cites W2168033818 @default.
- W2127248031 cites W2169792163 @default.
- W2127248031 cites W2178647298 @default.
- W2127248031 cites W2180487778 @default.
- W2127248031 cites W2187342692 @default.