FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2127281632> ?p ?o ?g. }

• W2127281632 endingPage "225" @default.
• W2127281632 startingPage "213" @default.
• W2127281632 abstract "We characterized inward rectifier (KIR) currents in reactive astrocytes activated by CNS injury. We used primary cultures of reactive astrocytes obtained from gelatin sponge implants in adult rat brains, a system that yielded highly purified, homogeneous cultures with >95% of cells positive for GFAP, vimentin, and S-100β. Ionic channels were studied in 1–21-day-old primary cultures using a nystatin-perforated patch clamp technique. Fast Na+ currents were identified in <2% of cells. Most cells exhibited outward currents positive to −50 mV, with one component being sensitive to charybdotoxin, iberiotoxin, and tetraethylammonium chloride, and another component being sensitive to 4-aminopyridine. Two populations of cells were distinguished, based on presence or absence of Ba2+-sensitive KIR current negative to the K+ reversal potential (Ek), with >80% of cells expressing KIR currents. In contrast to previous reports on mammalian astrocytes, the current-voltage curve showed no appreciable current between Ek and −50 mV, reflecting strong rectification by KIR channels. The magnitude of KIR current at −130 mV (I−130) did not change significantly during 21 days in culture (123 cells), suggesting constitutive expression of KIR channels. The fraction of KIR-negative cells was not affected by serum-starvation for 16–24 h. In cells with I−130 ≥ −30 pA, the membrane potential was invariably near Ek and depolarized appreciably on addition of Ba2+, but in cells with I−130 < −30 pA, resting potentials ranged from −40 mV to −90 mV. We conclude that most adult reactive astrocytes constitutively express KIR channel(s) that exhibit strong rectification not previously observed in mammalian astrocytes. GLIA 27:213–225, 1999. © 1999 Wiley-Liss, Inc." @default.
• W2127281632 created "2016-06-24" @default.
• W2127281632 creator A5025751238 @default.
• W2127281632 creator A5032122274 @default.
• W2127281632 creator A5073844022 @default.
• W2127281632 date "1999-09-01" @default.
• W2127281632 modified "2023-10-11" @default.
• W2127281632 title "K+ inward rectifier currents in reactive astrocytes from adult rat brain" @default.
• W2127281632 cites W1445544398 @default.
• W2127281632 cites W1502290520 @default.
• W2127281632 cites W1826049628 @default.
• W2127281632 cites W1864765563 @default.
• W2127281632 cites W1966242116 @default.
• W2127281632 cites W1969613932 @default.
• W2127281632 cites W1971213127 @default.
• W2127281632 cites W1972355909 @default.
• W2127281632 cites W1972362243 @default.
• W2127281632 cites W1982954912 @default.
• W2127281632 cites W1984746596 @default.
• W2127281632 cites W1986748876 @default.
• W2127281632 cites W1991143203 @default.
• W2127281632 cites W1993029198 @default.
• W2127281632 cites W1993191598 @default.
• W2127281632 cites W2000112012 @default.
• W2127281632 cites W2006207066 @default.
• W2127281632 cites W2006289803 @default.
• W2127281632 cites W2009667219 @default.
• W2127281632 cites W2012290561 @default.
• W2127281632 cites W2012968262 @default.
• W2127281632 cites W2013781469 @default.
• W2127281632 cites W2015754274 @default.
• W2127281632 cites W2016282258 @default.
• W2127281632 cites W2022231093 @default.
• W2127281632 cites W2026886935 @default.
• W2127281632 cites W2031418289 @default.
• W2127281632 cites W2044995558 @default.
• W2127281632 cites W2046663174 @default.
• W2127281632 cites W2047225757 @default.
• W2127281632 cites W2048336419 @default.
• W2127281632 cites W2049477374 @default.
• W2127281632 cites W2051953163 @default.
• W2127281632 cites W2052299467 @default.
• W2127281632 cites W2055390759 @default.
• W2127281632 cites W2057689655 @default.
• W2127281632 cites W2059989940 @default.
• W2127281632 cites W2059994807 @default.
• W2127281632 cites W2067605764 @default.
• W2127281632 cites W2068685899 @default.
• W2127281632 cites W2072225758 @default.
• W2127281632 cites W2072672589 @default.
• W2127281632 cites W2073426624 @default.
• W2127281632 cites W2080042147 @default.
• W2127281632 cites W2082304034 @default.
• W2127281632 cites W2084761937 @default.
• W2127281632 cites W2089761854 @default.
• W2127281632 cites W2125821357 @default.
• W2127281632 cites W2127138727 @default.
• W2127281632 cites W2147997339 @default.
• W2127281632 cites W2156677955 @default.
• W2127281632 cites W2160902428 @default.
• W2127281632 cites W2163161737 @default.
• W2127281632 cites W2164554425 @default.
• W2127281632 cites W2169931873 @default.
• W2127281632 cites W2258821072 @default.
• W2127281632 cites W2278495047 @default.
• W2127281632 cites W2311237560 @default.
• W2127281632 cites W4238401914 @default.
• W2127281632 cites W4319720330 @default.
• W2127281632 doi "https://doi.org/10.1002/(sici)1098-1136(199909)27:3<213::aid-glia3>3.0.co;2-1" @default.
• W2127281632 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10457368" @default.
• W2127281632 hasPublicationYear "1999" @default.
• W2127281632 type Work @default.
• W2127281632 sameAs 2127281632 @default.
• W2127281632 citedByCount "17" @default.
• W2127281632 countsByYear W21272816322012 @default.
• W2127281632 countsByYear W21272816322015 @default.
• W2127281632 crossrefType "journal-article" @default.
• W2127281632 hasAuthorship W2127281632A5025751238 @default.
• W2127281632 hasAuthorship W2127281632A5032122274 @default.
• W2127281632 hasAuthorship W2127281632A5073844022 @default.
• W2127281632 hasConcept C105702510 @default.
• W2127281632 hasConcept C12554922 @default.
• W2127281632 hasConcept C134018914 @default.
• W2127281632 hasConcept C147944092 @default.
• W2127281632 hasConcept C158494493 @default.
• W2127281632 hasConcept C169760540 @default.
• W2127281632 hasConcept C170493617 @default.
• W2127281632 hasConcept C178790620 @default.
• W2127281632 hasConcept C181911157 @default.
• W2127281632 hasConcept C185263204 @default.
• W2127281632 hasConcept C185592680 @default.
• W2127281632 hasConcept C2777542381 @default.
• W2127281632 hasConcept C2778071365 @default.
• W2127281632 hasConcept C2779700409 @default.
• W2127281632 hasConcept C2780419564 @default.
• W2127281632 hasConcept C2780918503 @default.
• W2127281632 hasConcept C2781295685 @default.
• W2127281632 hasConcept C4141045 @default.

• next