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- W2127372435 endingPage "5760" @default.
- W2127372435 startingPage "5749" @default.
- W2127372435 abstract "Entry into mitosis is catalyzed by cdc2 kinase. Previous work identified the cdc2-activating phosphatase cdc25C and the cdc2-inhibitory kinase wee1 as targets of the incomplete replication-induced kinase Chk1. Further work led to the model that checkpoint kinases block mitotic entry by inhibiting cdc25C through phosphorylation on Ser287 and activating wee1 through phosphorylation on Ser549. However, almost all conclusions underlying this idea were drawn from work using recombinant proteins. Here, we report that in the early Xenopus egg cell cycles, phosphorylation of endogenous cdc25C Ser287 is normally high during interphase and shows no obvious increase after checkpoint activation. By contrast, endogenous wee1 Ser549 phosphorylation is low during interphase and increases after activation of either the DNA damage or replication checkpoints; this is accompanied by a slight increase in wee1 kinase activity. Blocking mitotic entry by adding the catalytic subunit of PKA also results in increased wee1 Ser549 phosphorylation and maintenance of cdc25C Ser287 phosphorylation. These results argue that in response to checkpoint activation, endogenous wee1 is indeed a critical responder that functions by repressing the cdc2-cdc25C positive feedback loop. Surprisingly, endogenous wee1 Ser549 phosphorylation is highest during mitosis just after the peak of cdc2 activity. Treatments that block inactivation of cdc2 result in further increases in wee1 Ser549 phosphorylation, suggesting a previously unsuspected role for wee1 in mitosis." @default.
- W2127372435 created "2016-06-24" @default.
- W2127372435 creator A5040169264 @default.
- W2127372435 creator A5090752514 @default.
- W2127372435 date "2005-12-01" @default.
- W2127372435 modified "2023-09-27" @default.
- W2127372435 title "Changes in Regulatory Phosphorylation of Cdc25C Ser287 and Wee1 Ser549 during Normal Cell Cycle Progression and Checkpoint Arrests" @default.
- W2127372435 cites W144470551 @default.
- W2127372435 cites W1494248298 @default.
- W2127372435 cites W1500562904 @default.
- W2127372435 cites W1501575423 @default.
- W2127372435 cites W1520947267 @default.
- W2127372435 cites W1524628399 @default.
- W2127372435 cites W1543273979 @default.
- W2127372435 cites W1554610725 @default.
- W2127372435 cites W1558906202 @default.
- W2127372435 cites W1599979091 @default.
- W2127372435 cites W1677118171 @default.
- W2127372435 cites W171936907 @default.
- W2127372435 cites W1744830096 @default.
- W2127372435 cites W1882231298 @default.
- W2127372435 cites W1914194999 @default.
- W2127372435 cites W1925896202 @default.
- W2127372435 cites W192673857 @default.
- W2127372435 cites W1963838671 @default.
- W2127372435 cites W1964511352 @default.
- W2127372435 cites W1964970359 @default.
- W2127372435 cites W1967938513 @default.
- W2127372435 cites W1968268257 @default.
- W2127372435 cites W1969562119 @default.
- W2127372435 cites W1974861254 @default.
- W2127372435 cites W1980294824 @default.
- W2127372435 cites W1981772689 @default.
- W2127372435 cites W1981774463 @default.
- W2127372435 cites W1982037319 @default.
- W2127372435 cites W1982607151 @default.
- W2127372435 cites W1988876917 @default.
- W2127372435 cites W1990390216 @default.
- W2127372435 cites W1991444138 @default.
- W2127372435 cites W1995294841 @default.
- W2127372435 cites W2004313732 @default.
- W2127372435 cites W2010414017 @default.
- W2127372435 cites W2012120238 @default.
- W2127372435 cites W2014445311 @default.
- W2127372435 cites W2016274030 @default.
- W2127372435 cites W2021509469 @default.
- W2127372435 cites W2029479878 @default.
- W2127372435 cites W2031557677 @default.
- W2127372435 cites W2039144927 @default.
- W2127372435 cites W2041266127 @default.
- W2127372435 cites W2045876990 @default.
- W2127372435 cites W2050039745 @default.
- W2127372435 cites W2050123232 @default.
- W2127372435 cites W2058262532 @default.
- W2127372435 cites W2062541978 @default.
- W2127372435 cites W2064919643 @default.
- W2127372435 cites W2070632817 @default.
- W2127372435 cites W2072385327 @default.
- W2127372435 cites W2073808766 @default.
- W2127372435 cites W2074633316 @default.
- W2127372435 cites W2075532350 @default.
- W2127372435 cites W2076314401 @default.
- W2127372435 cites W2078142614 @default.
- W2127372435 cites W2083154558 @default.
- W2127372435 cites W2087697646 @default.
- W2127372435 cites W2093877987 @default.
- W2127372435 cites W2097917250 @default.
- W2127372435 cites W2101173071 @default.
- W2127372435 cites W2102752109 @default.
- W2127372435 cites W2103143169 @default.
- W2127372435 cites W2105016405 @default.
- W2127372435 cites W2105592350 @default.
- W2127372435 cites W2107389581 @default.
- W2127372435 cites W2109408011 @default.
- W2127372435 cites W2111423678 @default.
- W2127372435 cites W2126686878 @default.
- W2127372435 cites W2126960106 @default.
- W2127372435 cites W2127849733 @default.
- W2127372435 cites W2130290124 @default.
- W2127372435 cites W2136277289 @default.
- W2127372435 cites W2136614026 @default.
- W2127372435 cites W2137922559 @default.
- W2127372435 cites W2143156516 @default.
- W2127372435 cites W2143404342 @default.
- W2127372435 cites W2147220557 @default.
- W2127372435 cites W2167193338 @default.
- W2127372435 cites W2168259899 @default.
- W2127372435 cites W2171251720 @default.
- W2127372435 cites W2187534084 @default.
- W2127372435 cites W2334440180 @default.
- W2127372435 cites W2498326899 @default.
- W2127372435 cites W296218498 @default.
- W2127372435 cites W41535097 @default.
- W2127372435 cites W4290260729 @default.
- W2127372435 cites W63001887 @default.
- W2127372435 cites W935855719 @default.
- W2127372435 doi "https://doi.org/10.1091/mbc.e05-06-0541" @default.
- W2127372435 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1289418" @default.