FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2127478904> ?p ?o ?g. }

• W2127478904 endingPage "753" @default.
• W2127478904 startingPage "750" @default.
• W2127478904 abstract "To evaluate a technique for culture of Helicobacter pylori in large quantities of liquid media and to determine the factors that could influence the results.Fifteen clinical isolates of H pylori and a reference strain of H pylori NCTC11637 were used to evaluate a method to cultivate the organism in 100 ml liquid medium comprising brain heart infusion broth with 5% horse serum and 0.25% yeast extract. Tissue culture flasks containing the inoculated liquid medium were placed in a CO2 incubator with 5% CO2 for 2 hours and then incubated in a shaking incubator at 120 rpm.All the clinical isolates and the reference strain grew in the broth, although only a moderate growth of the reference strain occurred. Inoculum size significantly influenced the kinetics of growth of H pylori in the liquid medium. Vancomycin, nalidixic acid, and amphotericin B, used to suppress contamination, did not affect growth of H pylori in the medium. CO2 was essential for H pylori to grow or survive in the liquid medium. Incubation with CO2 in a CO2 incubator for 30 minutes or 2 hours did not affect the results.H pylori can be cultivated in large quantities of brain heart infusion broth with 5% horse serum and 0.25% yeast extract. Initial inoculum concentrations influence the kinetics of H pylori growth in the liquid medium. Vancomycin, nalidixic acid, and amphotericin B can be used as selective antimicrobial agents. CO2 is essential for initial growth of H pylori in liquid media. The findings in this study may provide a useful, reproducible, and simple method for biochemical, molecular, and physiological studies of H pylori, when those require large quantities of the organism." @default.
• W2127478904 created "2016-06-24" @default.
• W2127478904 creator A5029412958 @default.
• W2127478904 creator A5077320760 @default.
• W2127478904 creator A5085662381 @default.
• W2127478904 creator A5086710487 @default.
• W2127478904 date "1993-08-01" @default.
• W2127478904 modified "2023-09-26" @default.
• W2127478904 title "Enhanced cultivation of Helicobacter pylori in liquid media." @default.
• W2127478904 cites W1489691634 @default.
• W2127478904 cites W1510177956 @default.
• W2127478904 cites W1523662718 @default.
• W2127478904 cites W1835489049 @default.
• W2127478904 cites W1838381970 @default.
• W2127478904 cites W1862122604 @default.
• W2127478904 cites W1895981287 @default.
• W2127478904 cites W2005124357 @default.
• W2127478904 cites W2009428792 @default.
• W2127478904 cites W2041805992 @default.
• W2127478904 cites W2103933842 @default.
• W2127478904 cites W2135972635 @default.
• W2127478904 cites W2137795821 @default.
• W2127478904 cites W2401803952 @default.
• W2127478904 cites W2414650534 @default.
• W2127478904 doi "https://doi.org/10.1136/jcp.46.8.750" @default.
• W2127478904 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/501463" @default.
• W2127478904 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8408702" @default.
• W2127478904 hasPublicationYear "1993" @default.
• W2127478904 type Work @default.
• W2127478904 sameAs 2127478904 @default.
• W2127478904 citedByCount "31" @default.
• W2127478904 countsByYear W21274789042012 @default.
• W2127478904 countsByYear W21274789042013 @default.
• W2127478904 countsByYear W21274789042017 @default.
• W2127478904 crossrefType "journal-article" @default.
• W2127478904 hasAuthorship W2127478904A5029412958 @default.
• W2127478904 hasAuthorship W2127478904A5077320760 @default.
• W2127478904 hasAuthorship W2127478904A5085662381 @default.
• W2127478904 hasAuthorship W2127478904A5086710487 @default.
• W2127478904 hasBestOaLocation W21274789041 @default.
• W2127478904 hasConcept C100544194 @default.
• W2127478904 hasConcept C145890019 @default.
• W2127478904 hasConcept C17741926 @default.
• W2127478904 hasConcept C180032290 @default.
• W2127478904 hasConcept C185592680 @default.
• W2127478904 hasConcept C203014093 @default.
• W2127478904 hasConcept C21905920 @default.
• W2127478904 hasConcept C25642318 @default.
• W2127478904 hasConcept C2775876089 @default.
• W2127478904 hasConcept C2776021129 @default.
• W2127478904 hasConcept C2776409635 @default.
• W2127478904 hasConcept C2778660310 @default.
• W2127478904 hasConcept C2778980435 @default.
• W2127478904 hasConcept C2779050283 @default.
• W2127478904 hasConcept C2779489039 @default.
• W2127478904 hasConcept C2779548794 @default.
• W2127478904 hasConcept C2779629538 @default.
• W2127478904 hasConcept C2780919596 @default.
• W2127478904 hasConcept C2991793871 @default.
• W2127478904 hasConcept C31903555 @default.
• W2127478904 hasConcept C43617362 @default.
• W2127478904 hasConcept C501593827 @default.
• W2127478904 hasConcept C523546767 @default.
• W2127478904 hasConcept C54355233 @default.
• W2127478904 hasConcept C55493867 @default.
• W2127478904 hasConcept C86803240 @default.
• W2127478904 hasConcept C89423630 @default.
• W2127478904 hasConceptScore W2127478904C100544194 @default.
• W2127478904 hasConceptScore W2127478904C145890019 @default.
• W2127478904 hasConceptScore W2127478904C17741926 @default.
• W2127478904 hasConceptScore W2127478904C180032290 @default.
• W2127478904 hasConceptScore W2127478904C185592680 @default.
• W2127478904 hasConceptScore W2127478904C203014093 @default.
• W2127478904 hasConceptScore W2127478904C21905920 @default.
• W2127478904 hasConceptScore W2127478904C25642318 @default.
• W2127478904 hasConceptScore W2127478904C2775876089 @default.
• W2127478904 hasConceptScore W2127478904C2776021129 @default.
• W2127478904 hasConceptScore W2127478904C2776409635 @default.
• W2127478904 hasConceptScore W2127478904C2778660310 @default.
• W2127478904 hasConceptScore W2127478904C2778980435 @default.
• W2127478904 hasConceptScore W2127478904C2779050283 @default.
• W2127478904 hasConceptScore W2127478904C2779489039 @default.
• W2127478904 hasConceptScore W2127478904C2779548794 @default.
• W2127478904 hasConceptScore W2127478904C2779629538 @default.
• W2127478904 hasConceptScore W2127478904C2780919596 @default.
• W2127478904 hasConceptScore W2127478904C2991793871 @default.
• W2127478904 hasConceptScore W2127478904C31903555 @default.
• W2127478904 hasConceptScore W2127478904C43617362 @default.
• W2127478904 hasConceptScore W2127478904C501593827 @default.
• W2127478904 hasConceptScore W2127478904C523546767 @default.
• W2127478904 hasConceptScore W2127478904C54355233 @default.
• W2127478904 hasConceptScore W2127478904C55493867 @default.
• W2127478904 hasConceptScore W2127478904C86803240 @default.
• W2127478904 hasConceptScore W2127478904C89423630 @default.
• W2127478904 hasIssue "8" @default.
• W2127478904 hasLocation W21274789041 @default.
• W2127478904 hasLocation W21274789042 @default.
• W2127478904 hasLocation W21274789043 @default.

• next