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- W2127486529 startingPage "342" @default.
- W2127486529 abstract "The rate of spontaneous lipid peroxidation, as measured by formation of malonaldehyde (MA), was determined as a function of O2 concentration and temperature in mouse and rabbit spermatozoa released from the cauda epididymidis. The peroxidation rate was linear in O2 concentration in the suspending medium up to 210 μM (the concentration at PO2 of ambient air at 34°C) for sperm from both species over the temperature range 34–40°C. This is the range over which the reaction is measurable for both species: below 34°C, the rates become too slow to be measured accurately for rabbit sperm by our methods, while above 40°C the rates for mouse sperm become too rapid. This narrow range is characteristic of a high activation energy (EA) for the peroxidation process. Values of EA were calculated from plots of kox versus (T)−1, where kox is a second order rate constant with the units (108 cells/ml)−1 min−1. It is defined by the equation: vma = kox (Sp) (O2), where vma is the rate of malonaldehyde production, (Sp) is concentration of sperm cells and (O2) is the O2 concentration in the suspending medium. For mouse sperm, EA was calculated to 78.7 kcal/mol (329 KJ/mol); for rabbit sperm, the value was 77.6 kcal/ml (324 KJ/mol). These high EAs and consequent steep dependence of the spontaneous lipid peroxidation rates on temperature favor long sperm life in the epididymis at around 32°C and low PO2 in these scrotal animals, while allowing for a relatively short life at 37°C at higher PO2 in the oviduct." @default.
- W2127486529 created "2016-06-24" @default.
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- W2127486529 date "1985-03-01" @default.
- W2127486529 modified "2023-09-27" @default.
- W2127486529 title "Spontaneous Lipid Peroxidation in Rabbit and Mouse Epididymal Spermatozoa: Dependence of Rate on Temperature and Oxygen Concentration" @default.
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- W2127486529 doi "https://doi.org/10.1095/biolreprod32.2.342" @default.
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