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• W2127629841 abstract "Research Article1 December 1990free access Splicing factor SF4 is dispensable for the assembly of a functional splicing complex and participates in the subsequent steps of the splicing reaction. U. Utans U. Utans Department of Cell Biology, University of Basel, Switzerland. Search for more papers by this author A. Krämer A. Krämer Department of Cell Biology, University of Basel, Switzerland. Search for more papers by this author U. Utans U. Utans Department of Cell Biology, University of Basel, Switzerland. Search for more papers by this author A. Krämer A. Krämer Department of Cell Biology, University of Basel, Switzerland. Search for more papers by this author Author Information U. Utans1 and A. Krämer1 1Department of Cell Biology, University of Basel, Switzerland. The EMBO Journal (1990)9:4119-4126https://doi.org/10.1002/j.1460-2075.1990.tb07634.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The splicing of nuclear messenger RNA precursors (pre-mRNA) can be reconstituted in vitro with factors partially purified from HeLa cell nuclear extracts. Splicing complexes are assembled in the presence of the small nuclear ribonucleoprotein particles (snRNPs) U1, U2, U4, U5 and U6 and the protein factors SF1, SF2, SF3 and U2AF. However, the complexes thus formed are inactive, i.e. they only contain unprocessed pre-mRNA. The intermediates and products of the splicing reaction are generated after addition of SF4. This splicing factor is a heat-labile protein which requires sulfhydryl groups for its activity. SF4 appears to participate, directly or indirectly, in the conversion of a functional but inactive splicing complex to the active spliceosome. Previous ArticleNext Article Volume 9Issue 121 December 1990In this issue RelatedDetailsLoading ..." @default.
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• W2127629841 date "1990-12-01" @default.
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• W2127629841 title "Splicing factor SF4 is dispensable for the assembly of a functional splicing complex and participates in the subsequent steps of the splicing reaction." @default.
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• W2127629841 doi "https://doi.org/10.1002/j.1460-2075.1990.tb07634.x" @default.
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