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• W2127691804 endingPage "3359" @default.
• W2127691804 startingPage "3355" @default.
• W2127691804 abstract "Both homologous and nonhomologous recombination events occur at high efficiency in DNA molecules transfected into mammalian cells. Both types of recombination occur with similar overall efficiencies, as measured by an endpoint assay, but their relative rates are unknown. In this communication, we measure the relative rates of homologous and nonhomologous recombination in DNA transfected into monkey cells. This measurement is made by using a linear simian virus 40 genome that contains a 131-base-pair duplication at its termini. Once inside the cell, this molecule must circularize to initiate lytic infection. Circularization can occur either by direct, nonhomologous end-joining or by homologous recombination within the duplicated region. Although the products of the two recombination pathways are different, they are equally infectious. Since homologous and nonhomologous recombination processes are competing for the same substrate, the relative amounts of the products of each pathway should reflect the relative rates of homologous and nonhomologous recombination. Analysis of individual recombinant genomes from 164 plaques indicates that the rate of circularization by nonhomologous recombination is 2- to 3-fold higher than the rate of homologous recombination. The assay system described here may prove to be useful for testing procedures designed to influence the relative rates of homologous and nonhomologous recombination." @default.
• W2127691804 created "2016-06-24" @default.
• W2127691804 creator A5049475981 @default.
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• W2127691804 date "1985-05-01" @default.
• W2127691804 modified "2023-10-18" @default.
• W2127691804 title "Relative rates of homologous and nonhomologous recombination in transfected DNA." @default.
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• W2127691804 doi "https://doi.org/10.1073/pnas.82.10.3355" @default.
• W2127691804 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/397774" @default.
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• W2127691804 hasPublicationYear "1985" @default.
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