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• W2127726997 endingPage "5070" @default.
• W2127726997 startingPage "5070" @default.
• W2127726997 abstract "Secretory leukocyte peptidase inhibitors (SLPI), elafin, squamous cell carcinoma antigen 1 and 2 (SCCA1 and SCCA2) are specific endogenous serine protease inhibitors expressed by epithelial cells that prevent tissue damage from excessive proteolytic enzyme activity due to inflammation. To determine the effects of various periopathogens on these protease inhibitors, we utilized human gingival epithelial cells (GECs) challenged with cell-free bacteria supernatants of various periopathogens Porphyromonas gingivalis, Tannerella forsythia, Aggregatibacter actinomycetemcomitans, and Fusobacterium nucleatum.The gene expression and secretion of SLPI, elafin, SCCA1, and SCCA2 were determined using real-time PCR and ELISA, respectively. The direct effects of periopathogens and P. gingivalis gingipain mutants on these inhibitors were examined in vitro by Western Blot. The effect on the innate immune response of GECs was measured by expression of antimicrobial peptides: human beta-defenisin-2 (hBD2) and chemokine (C-C motif) ligand 20 (CCL20).We found that SLPI, SCCA2, elafin, hBD2, and CCL20 gene expression levels were significantly induced (p<0.001) in response to P. gingivalis, whose virulence factors include cysteine proteases, but not in response to stimulation by other bacteria. P. gingivalis reduced the secretion of SLPI and elafin significantly in GECs, and degraded recombinant SLPI, elafin, SCCA1, and SCCA2. Differential degradation patterns of SLPI, elafin, SCCA1, and SCCA2 were observed with different bacteria as well as P. gingivalis mutants associated with the loss of specific gingipains secreted by P. gingivalis. In addition, pretreatment of GECs with SLPI, SCCA1, or SCCA2 partially blocked hBD2 and CCL20 mRNA expression in response to P. gingivalis, suggesting a protective effect.Our results suggest that different periopathogens affect the host protease inhibitors in a different manner, suggesting host susceptibility may differ in the presence of these pathogens. The balance between cellular protease inhibitors and their degradation may be an important factor in susceptibility to periodontal infection." @default.
• W2127726997 created "2016-06-24" @default.
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• W2127726997 date "2010-01-01" @default.
• W2127726997 modified "2023-10-17" @default.
• W2127726997 title "Differential effects of periopathogens on host protease inhibitors SLPI, elafin, SCCA1, and SCCA2" @default.
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• W2127726997 cites W1967433137 @default.
• W2127726997 cites W1974659792 @default.
• W2127726997 cites W1981004429 @default.
• W2127726997 cites W1983562760 @default.
• W2127726997 cites W1984276265 @default.
• W2127726997 cites W1985233421 @default.
• W2127726997 cites W1994999799 @default.
• W2127726997 cites W1999180967 @default.
• W2127726997 cites W1999564888 @default.
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• W2127726997 cites W2024249553 @default.
• W2127726997 cites W2024428337 @default.
• W2127726997 cites W2031965552 @default.
• W2127726997 cites W2032054488 @default.
• W2127726997 cites W2034352127 @default.
• W2127726997 cites W2039643144 @default.
• W2127726997 cites W2054724293 @default.
• W2127726997 cites W2061520837 @default.
• W2127726997 cites W2061766973 @default.
• W2127726997 cites W2064651323 @default.
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• W2127726997 cites W2088258179 @default.
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• W2127726997 cites W2095371879 @default.
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• W2127726997 cites W2109696752 @default.
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• W2127726997 doi "https://doi.org/10.3402/jom.v2i0.5070" @default.
• W2127726997 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3084571" @default.
• W2127726997 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21523231" @default.
• W2127726997 hasPublicationYear "2010" @default.
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