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• W2127730346 abstract "Spiramycin, a 16-membered macrolide antibiotic used in human medicine, is produced by Streptomyces ambofaciens; it comprises a polyketide lactone, platenolide, to which three deoxyhexose sugars are attached. In order to characterize the gene cluster governing the biosynthesis of spiramycin, several overlapping cosmids were isolated from an S. ambofaciens gene library, by hybridization with various probes (spiramycin resistance or biosynthetic genes, tylosin biosynthetic genes), and the sequences of their inserts were determined. Sequence analysis showed that the spiramycin biosynthetic gene cluster spanned a region of over 85 kb of contiguous DNA. In addition to the five previously described genes that encode the type I polyketide synthase involved in platenolide biosynthesis, 45 other genes have been identified. It was possible to propose a function for most of the inferred proteins in spiramycin biosynthesis, in its regulation, in resistance to the produced antibiotic or in the provision of extender units for the polyketide synthase. Two of these genes, predicted to be involved in deoxysugar biosynthesis, were inactivated by gene replacement, and the resulting mutants were unable to produce spiramycin, thus confirming their involvement in spiramycin biosynthesis. This work reveals the main features of spiramycin biosynthesis and constitutes a first step towards a detailed molecular analysis of the production of this medically important antibiotic." @default.
• W2127730346 created "2016-06-24" @default.
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• W2127730346 date "2007-12-01" @default.
• W2127730346 modified "2023-10-10" @default.
• W2127730346 title "Organization of the biosynthetic gene cluster for the macrolide antibiotic spiramycin in Streptomyces ambofaciens" @default.
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• W2127730346 cites W176996418 @default.
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• W2127730346 cites W1970712032 @default.
• W2127730346 cites W1971163671 @default.
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• W2127730346 cites W1985217466 @default.
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• W2127730346 cites W1998307795 @default.
• W2127730346 cites W2002684744 @default.
• W2127730346 cites W2004589024 @default.
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• W2127730346 cites W2063952111 @default.
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• W2127730346 doi "https://doi.org/10.1099/mic.0.2007/009746-0" @default.
• W2127730346 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18048924" @default.
• W2127730346 hasPublicationYear "2007" @default.
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