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• W2127754468 abstract "By making site-directed mutations in the avian retroviral oncogene v-rel, we created two temperature-sensitive (ts) transforming mutants; these changes were analogous to mutations previously shown to confer a ts function onto the Dorsal protein of Drosophila melanogaster. Chicken spleen cells infected with the ts v-rel mutants formed colonies in agar at 36.5 degrees C but not at 41.5 degrees C. In addition, spleen cells derived from the ts v-rel-transformed colonies could be propagated in liquid culture at 36.5 degrees C but rapidly senesced at 41.5 degrees C. Both mutant v-Rel proteins were also ts for DNA binding in vitro. These mutants may be valuable for identifying genes directly regulated by v-rel." @default.
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• W2127754468 date "1993-11-01" @default.
• W2127754468 modified "2023-10-16" @default.
• W2127754468 title "Temperature-sensitive transforming mutants of the v-rel oncogene" @default.
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• W2127754468 doi "https://doi.org/10.1128/jvi.67.11.6876-6881.1993" @default.
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