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- W2127757002 abstract "The availability of next-generation sequences of transcripts from prokaryotic organisms offers the opportunity to design a new generation of automated genome annotation tools not yet available for prokaryotes. In this work, we designed EuGene-P, the first integrative prokaryotic gene finder tool which combines a variety of high-throughput data, including oriented RNA-Seq data, directly into the prediction process. This enables the automated prediction of coding sequences (CDSs), untranslated regions, transcription start sites (TSSs) and non-coding RNA (ncRNA, sense and antisense) genes. EuGene-P was used to comprehensively and accurately annotate the genome of the nitrogen-fixing bacterium Sinorhizobium meliloti strain 2011, leading to the prediction of 6308 CDSs as well as 1876 ncRNAs. Among them, 1280 appeared as antisense to a CDS, which supports recent findings that antisense transcription activity is widespread in bacteria. Moreover, 4077 TSSs upstream of protein-coding or non-coding genes were precisely mapped providing valuable data for the study of promoter regions. By looking for RpoE2-binding sites upstream of annotated TSSs, we were able to extend the S. meliloti RpoE2 regulon by ∼3-fold. Altogether, these observations demonstrate the power of EuGene-P to produce a reliable and high-resolution automatic annotation of prokaryotic genomes." @default.
- W2127757002 created "2016-06-24" @default.
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- W2127757002 date "2013-04-18" @default.
- W2127757002 modified "2023-10-14" @default.
- W2127757002 title "Next-Generation Annotation of Prokaryotic Genomes with EuGene-P: Application to Sinorhizobium meliloti 2011" @default.
- W2127757002 cites W1530687839 @default.
- W2127757002 cites W1582203190 @default.
- W2127757002 cites W1585170874 @default.
- W2127757002 cites W1608640243 @default.
- W2127757002 cites W1772414580 @default.
- W2127757002 cites W1864542181 @default.
- W2127757002 cites W1868948876 @default.
- W2127757002 cites W1880436856 @default.
- W2127757002 cites W1966786968 @default.
- W2127757002 cites W1975277828 @default.
- W2127757002 cites W1976008137 @default.
- W2127757002 cites W1977828801 @default.
- W2127757002 cites W1978480609 @default.
- W2127757002 cites W1989895641 @default.
- W2127757002 cites W1994901531 @default.
- W2127757002 cites W1996974387 @default.
- W2127757002 cites W2004548026 @default.
- W2127757002 cites W2005721307 @default.
- W2127757002 cites W2010670581 @default.
- W2127757002 cites W2016170819 @default.
- W2127757002 cites W2022414939 @default.
- W2127757002 cites W2023270843 @default.
- W2127757002 cites W2024162926 @default.
- W2127757002 cites W2024349866 @default.
- W2127757002 cites W2025339718 @default.
- W2127757002 cites W2030445845 @default.
- W2127757002 cites W2034164729 @default.
- W2127757002 cites W2039539454 @default.
- W2127757002 cites W2039779035 @default.
- W2127757002 cites W2040100578 @default.
- W2127757002 cites W2046539662 @default.
- W2127757002 cites W2054933558 @default.
- W2127757002 cites W2059513932 @default.
- W2127757002 cites W2062896692 @default.
- W2127757002 cites W2063327774 @default.
- W2127757002 cites W2066114727 @default.
- W2127757002 cites W2075960471 @default.
- W2127757002 cites W2077460182 @default.
- W2127757002 cites W2078609524 @default.
- W2127757002 cites W2080693057 @default.
- W2127757002 cites W2082644333 @default.
- W2127757002 cites W2086240273 @default.
- W2127757002 cites W2092779913 @default.
- W2127757002 cites W2096549168 @default.
- W2127757002 cites W2098909650 @default.
- W2127757002 cites W2102637097 @default.
- W2127757002 cites W2105199251 @default.
- W2127757002 cites W2105423509 @default.
- W2127757002 cites W2107915254 @default.
- W2127757002 cites W2109457118 @default.
- W2127757002 cites W2112451705 @default.
- W2127757002 cites W2113296099 @default.
- W2127757002 cites W2116093291 @default.
- W2127757002 cites W2117843745 @default.
- W2127757002 cites W2118072443 @default.
- W2127757002 cites W2118522962 @default.
- W2127757002 cites W2118771542 @default.
- W2127757002 cites W2118970397 @default.
- W2127757002 cites W2120799151 @default.
- W2127757002 cites W2121891040 @default.
- W2127757002 cites W2125591244 @default.
- W2127757002 cites W2127820561 @default.
- W2127757002 cites W2128889957 @default.
- W2127757002 cites W2131330686 @default.
- W2127757002 cites W2133253129 @default.
- W2127757002 cites W2134864570 @default.
- W2127757002 cites W2137692880 @default.
- W2127757002 cites W2141847640 @default.
- W2127757002 cites W2143485490 @default.
- W2127757002 cites W2145385227 @default.
- W2127757002 cites W2152045787 @default.
- W2127757002 cites W2152239989 @default.
- W2127757002 cites W2152519010 @default.
- W2127757002 cites W2153268880 @default.
- W2127757002 cites W2153451605 @default.
- W2127757002 cites W2156095709 @default.
- W2127757002 cites W2157915336 @default.
- W2127757002 cites W2159880962 @default.
- W2127757002 cites W2161666385 @default.
- W2127757002 cites W2163403059 @default.
- W2127757002 cites W2166494042 @default.