FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2127788740> ?p ?o ?g. }

• W2127788740 endingPage "99" @default.
• W2127788740 startingPage "90" @default.
• W2127788740 abstract "Background The 5α-reduced pregnane neuroactive steroid (3α,5α)-3-hydroxypregnan-20-one (3α,5α-THP or allopregnanolone) is a potent positive modulator of GABAA receptors capable of modulating neuronal activity. In rats, systemic ethanol (EtOH) administration increases cerebral cortical and hippocampal levels of 3α,5α-THP, but the effects of EtOH on 3α,5α-THP levels in other brain regions are unknown. There is a large body of evidence suggesting that 3α,5α-THP enhances EtOH sensitivity, contributes to some behavioral effects of EtOH, and modulates EtOH reinforcement and motivation to drink. In this study, we used immunohistochemistry (IHC) to determine EtOH-induced changes in cellular 3α,5α-THP expression in brain regions associated with EtOH actions and responses. Methods Male Wistar rats were administered EtOH (2 g/kg) or saline intraperitoneally and after 60 minutes transcardially perfused. IHC was performed on free-floating sections (3 to 4 sections/animal/brain region) using an affinity purified anti-3α,5α-THP primary antibody, and immunoreactivity was visualized with 3,3′-diaminobenzidine. Results EtOH significantly increased 3α,5α-THP immunoreactivity by 24 ± 6% in the medial prefrontal cortex, 32 ± 12% in the hippocampal Cornu Ammonis area 1 (CA1) pyramidal cell layer, 52 ± 5% in the polymorph cell layer of the dentate gyrus (DG), 44 ± 15% in the bed nucleus of the stria terminalis, and 36 ± 6% in the paraventricular nucleus of the hypothalamus. In contrast, EtOH administration significantly reduced 3α,5α-THP immunoreactivity by 25 ± 5% in the nucleus accumbens “shore” and 21 ± 3% in the central nucleus of the amygdala. No changes were observed in the ventral tegmental area, dorsomedial striatum, granule cell layer of the DG, or the lateral and basolateral amygdala. Conclusions The results suggest acute EtOH (2 g/kg) produces divergent, brain region specific, effects on cellular 3α,5α-THP levels. Regional differences in the effects of EtOH suggest there may be regional brain synthesis of 3α,5α-THP independent of the adrenal glands and novel mechanisms that reduce cellular 3α,5α-THP. Regional differences in EtOH-induced changes in 3α,5α-THP levels likely contribute to EtOH effects on neuronal function in brain." @default.
• W2127788740 created "2016-06-24" @default.
• W2127788740 creator A5024081434 @default.
• W2127788740 creator A5069274866 @default.
• W2127788740 creator A5076168985 @default.
• W2127788740 creator A5078096912 @default.
• W2127788740 date "2013-07-26" @default.
• W2127788740 modified "2023-10-16" @default.
• W2127788740 title "Ethanol Administration Produces Divergent Changes in GABAergic Neuroactive Steroid Immunohistochemistry in the Rat Brain" @default.
• W2127788740 cites W1550399699 @default.
• W2127788740 cites W1570477602 @default.
• W2127788740 cites W1642619469 @default.
• W2127788740 cites W1814943294 @default.
• W2127788740 cites W1922154348 @default.
• W2127788740 cites W1973115552 @default.
• W2127788740 cites W1976707885 @default.
• W2127788740 cites W1982298951 @default.
• W2127788740 cites W1991772069 @default.
• W2127788740 cites W1992576862 @default.
• W2127788740 cites W1998585216 @default.
• W2127788740 cites W2000712333 @default.
• W2127788740 cites W2004439638 @default.
• W2127788740 cites W2004846311 @default.
• W2127788740 cites W2008920256 @default.
• W2127788740 cites W2009295364 @default.
• W2127788740 cites W2012435835 @default.
• W2127788740 cites W2013773564 @default.
• W2127788740 cites W2017227716 @default.
• W2127788740 cites W2024350454 @default.
• W2127788740 cites W2029002531 @default.
• W2127788740 cites W2031351244 @default.
• W2127788740 cites W2031458111 @default.
• W2127788740 cites W2033500251 @default.
• W2127788740 cites W2037605765 @default.
• W2127788740 cites W2047673684 @default.
• W2127788740 cites W2050844190 @default.
• W2127788740 cites W2052511485 @default.
• W2127788740 cites W2054920627 @default.
• W2127788740 cites W2057387160 @default.
• W2127788740 cites W2058404259 @default.
• W2127788740 cites W2060043050 @default.
• W2127788740 cites W2061979625 @default.
• W2127788740 cites W2063059318 @default.
• W2127788740 cites W2065760031 @default.
• W2127788740 cites W2067260885 @default.
• W2127788740 cites W2072990514 @default.
• W2127788740 cites W2083257590 @default.
• W2127788740 cites W2084243951 @default.
• W2127788740 cites W2084775431 @default.
• W2127788740 cites W2110450709 @default.
• W2127788740 cites W2143178821 @default.
• W2127788740 cites W2159584029 @default.
• W2127788740 cites W2160829410 @default.
• W2127788740 cites W2163215105 @default.
• W2127788740 cites W4298239848 @default.
• W2127788740 cites W4322697749 @default.
• W2127788740 doi "https://doi.org/10.1111/acer.12223" @default.
• W2127788740 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4196317" @default.
• W2127788740 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23906006" @default.
• W2127788740 hasPublicationYear "2013" @default.
• W2127788740 type Work @default.
• W2127788740 sameAs 2127788740 @default.
• W2127788740 citedByCount "36" @default.
• W2127788740 countsByYear W21277887402014 @default.
• W2127788740 countsByYear W21277887402015 @default.
• W2127788740 countsByYear W21277887402016 @default.
• W2127788740 countsByYear W21277887402017 @default.
• W2127788740 countsByYear W21277887402018 @default.
• W2127788740 countsByYear W21277887402019 @default.
• W2127788740 countsByYear W21277887402020 @default.
• W2127788740 countsByYear W21277887402022 @default.
• W2127788740 crossrefType "journal-article" @default.
• W2127788740 hasAuthorship W2127788740A5024081434 @default.
• W2127788740 hasAuthorship W2127788740A5069274866 @default.
• W2127788740 hasAuthorship W2127788740A5076168985 @default.
• W2127788740 hasAuthorship W2127788740A5078096912 @default.
• W2127788740 hasBestOaLocation W21277887402 @default.
• W2127788740 hasConcept C126322002 @default.
• W2127788740 hasConcept C134018914 @default.
• W2127788740 hasConcept C148762608 @default.
• W2127788740 hasConcept C168258287 @default.
• W2127788740 hasConcept C170493617 @default.
• W2127788740 hasConcept C185592680 @default.
• W2127788740 hasConcept C204232928 @default.
• W2127788740 hasConcept C2776464000 @default.
• W2127788740 hasConcept C2776552330 @default.
• W2127788740 hasConcept C2776801781 @default.
• W2127788740 hasConcept C2777003273 @default.
• W2127788740 hasConcept C2778338918 @default.
• W2127788740 hasConcept C2778652331 @default.
• W2127788740 hasConcept C2780715579 @default.
• W2127788740 hasConcept C2781161787 @default.
• W2127788740 hasConcept C44796713 @default.
• W2127788740 hasConcept C62525196 @default.
• W2127788740 hasConcept C71924100 @default.
• W2127788740 hasConcept C86803240 @default.
• W2127788740 hasConceptScore W2127788740C126322002 @default.
• W2127788740 hasConceptScore W2127788740C134018914 @default.

• next