FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2127924870> ?p ?o ?g. }

• W2127924870 endingPage "2069" @default.
• W2127924870 startingPage "2059" @default.
• W2127924870 abstract "TREX1 is the major exonuclease in mammalian cells, exhibiting the highest level of activity with a 3'-->5' activity. This exonuclease is responsible in humans for Aicardi-Goutières syndrome and for an autosomal dominant retinal vasculopathy with cerebral leukodystrophy. In addition, this enzyme is associated with systemic lupus erythematosus. TREX1 belongs to the exonuclease DEDDh family, whose members display low levels of sequence identity, while possessing a common fold and active site organization. For these exonucleases, a catalytic mechanism has been proposed that involves two divalent metal ions bound to the DEDD motif. Here we studied the interaction of TREX1 with the monovalent cations lithium and sodium. We demonstrate that these metals inhibit the exonucleolytic activity of TREX1, as measured by the classical gel method, as well as by a new technique developed for monitoring the real-time exonuclease reaction. The X-ray structures of the enzyme in complex with these two cations and with a nucleotide, a product of the exonuclease reaction, were determined at 2.1 A and 2.3 A, respectively. A comparison with the structures of the active complexes (in the presence of magnesium or manganese) explains that the inhibition mechanism is caused by the noncatalytic metals competing with distinct affinities for the two metal-binding sites and inducing subtle rearrangements in active centers. Our analysis also reveals that a histidine residue (His124), highly conserved in the DEDDh family, is involved in the activity of TREX1, as confirmed by mutational studies. Our results shed further light on the mechanism of activity of the DEDEh family of exonucleases." @default.
• W2127924870 created "2016-06-24" @default.
• W2127924870 creator A5008527409 @default.
• W2127924870 creator A5024300705 @default.
• W2127924870 creator A5034077469 @default.
• W2127924870 creator A5039868810 @default.
• W2127924870 creator A5041780643 @default.
• W2127924870 creator A5078239155 @default.
• W2127924870 date "2008-12-01" @default.
• W2127924870 modified "2023-10-06" @default.
• W2127924870 title "Structural and biochemical studies of TREX1 inhibition by metals. Identification of a new active histidine conserved in DEDDh exonucleases" @default.
• W2127924870 cites W1494661462 @default.
• W2127924870 cites W1503256903 @default.
• W2127924870 cites W1509550144 @default.
• W2127924870 cites W1539796472 @default.
• W2127924870 cites W1583621573 @default.
• W2127924870 cites W1591131153 @default.
• W2127924870 cites W1969824361 @default.
• W2127924870 cites W1969888906 @default.
• W2127924870 cites W1972746334 @default.
• W2127924870 cites W1975769646 @default.
• W2127924870 cites W1976781795 @default.
• W2127924870 cites W1976862545 @default.
• W2127924870 cites W1986191025 @default.
• W2127924870 cites W1987216874 @default.
• W2127924870 cites W1988239052 @default.
• W2127924870 cites W1992897184 @default.
• W2127924870 cites W1996263865 @default.
• W2127924870 cites W1997327174 @default.
• W2127924870 cites W2019324992 @default.
• W2127924870 cites W2032842297 @default.
• W2127924870 cites W2036634480 @default.
• W2127924870 cites W2037632892 @default.
• W2127924870 cites W2041421251 @default.
• W2127924870 cites W2043959346 @default.
• W2127924870 cites W2062080272 @default.
• W2127924870 cites W2063020364 @default.
• W2127924870 cites W2063335878 @default.
• W2127924870 cites W2068992336 @default.
• W2127924870 cites W2070196801 @default.
• W2127924870 cites W2070677737 @default.
• W2127924870 cites W2075172390 @default.
• W2127924870 cites W2076690763 @default.
• W2127924870 cites W2086906135 @default.
• W2127924870 cites W2145812105 @default.
• W2127924870 cites W2146690493 @default.
• W2127924870 cites W2147019339 @default.
• W2127924870 cites W2158521317 @default.
• W2127924870 cites W2164340098 @default.
• W2127924870 cites W2171946670 @default.
• W2127924870 doi "https://doi.org/10.1110/ps.036426.108" @default.
• W2127924870 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2590924" @default.
• W2127924870 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18780819" @default.
• W2127924870 hasPublicationYear "2008" @default.
• W2127924870 type Work @default.
• W2127924870 sameAs 2127924870 @default.
• W2127924870 citedByCount "26" @default.
• W2127924870 countsByYear W21279248702012 @default.
• W2127924870 countsByYear W21279248702013 @default.
• W2127924870 countsByYear W21279248702014 @default.
• W2127924870 countsByYear W21279248702015 @default.
• W2127924870 countsByYear W21279248702017 @default.
• W2127924870 countsByYear W21279248702018 @default.
• W2127924870 countsByYear W21279248702019 @default.
• W2127924870 countsByYear W21279248702020 @default.
• W2127924870 countsByYear W21279248702021 @default.
• W2127924870 countsByYear W21279248702022 @default.
• W2127924870 crossrefType "journal-article" @default.
• W2127924870 hasAuthorship W2127924870A5008527409 @default.
• W2127924870 hasAuthorship W2127924870A5024300705 @default.
• W2127924870 hasAuthorship W2127924870A5034077469 @default.
• W2127924870 hasAuthorship W2127924870A5039868810 @default.
• W2127924870 hasAuthorship W2127924870A5041780643 @default.
• W2127924870 hasAuthorship W2127924870A5078239155 @default.
• W2127924870 hasBestOaLocation W21279248702 @default.
• W2127924870 hasConcept C107824862 @default.
• W2127924870 hasConcept C12554922 @default.
• W2127924870 hasConcept C178790620 @default.
• W2127924870 hasConcept C181199279 @default.
• W2127924870 hasConcept C185592680 @default.
• W2127924870 hasConcept C2777501222 @default.
• W2127924870 hasConcept C2778460671 @default.
• W2127924870 hasConcept C41183919 @default.
• W2127924870 hasConcept C54415193 @default.
• W2127924870 hasConcept C55493867 @default.
• W2127924870 hasConcept C71240020 @default.
• W2127924870 hasConcept C74884574 @default.
• W2127924870 hasConcept C82381507 @default.
• W2127924870 hasConcept C86803240 @default.
• W2127924870 hasConceptScore W2127924870C107824862 @default.
• W2127924870 hasConceptScore W2127924870C12554922 @default.
• W2127924870 hasConceptScore W2127924870C178790620 @default.
• W2127924870 hasConceptScore W2127924870C181199279 @default.
• W2127924870 hasConceptScore W2127924870C185592680 @default.
• W2127924870 hasConceptScore W2127924870C2777501222 @default.
• W2127924870 hasConceptScore W2127924870C2778460671 @default.
• W2127924870 hasConceptScore W2127924870C41183919 @default.
• W2127924870 hasConceptScore W2127924870C54415193 @default.

• next