FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2128009076> ?p ?o ?g. }

• W2128009076 endingPage "4538" @default.
• W2128009076 startingPage "4527" @default.
• W2128009076 abstract "Because the pathogenesis of enterovirus 71 (EV71) remains mostly ambiguous, identifying the factors that mediate viral binding and entry to host cells is indispensable to ultimately uncover the mechanisms that underlie virus infection and pathogenesis. Despite the identification of several receptors/attachment molecules for EV71, the binding, entry, and infection mechanisms of EV71 remain unclear. Herein, we employed glycoproteomic approaches to identify human nucleolin as a novel binding receptor for EV71. Glycoproteins purified by lectin chromatography from the membrane extraction of human cells were treated with sialidase, followed by immunoprecipitation with EV71 particles. Among the 16 proteins identified by tandem mass spectrometry analysis, cell surface nucleolin attracted our attention. We found that EV71 interacted directly with nucleolin via the VP1 capsid protein and that an antinucleolin antibody reduced the binding of EV71 to human cells. In addition, the knockdown of cell surface nucleolin decreased EV71 binding, infection, and production in human cells. Furthermore, the expression of human nucleolin on the cell surface of a mouse cell line increased EV71 binding and conferred EV71 infection and production in the cells. These results strongly indicate that human nucleolin can mediate EV71 binding to and infection of cells. Our findings also demonstrate that the use of glycoproteomic approaches is a reliable methodology to discover novel receptors for pathogens.Outbreaks of EV71 have been reported in Asia-Pacific countries and have caused thousands of deaths in young children during the last 2 decades. The discovery of new EV71-interacting molecules to understand the infection mechanism has become an emergent issue. Hence, this study uses glycoproteomic approaches to comprehensively investigate the EV71-interacting glycoproteins. Several EV71-interacting glycoproteins are identified, and the role of cell surface nucleolin in mediating the attachment and entry of EV71 is characterized and validated. Our findings not only indicate a novel target for uncovering the EV71 infection mechanism and anti-EV71 drug discovery but also provide a new strategy for virus receptor identification." @default.
• W2128009076 created "2016-06-24" @default.
• W2128009076 creator A5006218473 @default.
• W2128009076 creator A5007401599 @default.
• W2128009076 creator A5009319194 @default.
• W2128009076 creator A5016157527 @default.
• W2128009076 creator A5016600846 @default.
• W2128009076 creator A5057646751 @default.
• W2128009076 creator A5068654910 @default.
• W2128009076 creator A5075501159 @default.
• W2128009076 creator A5077247151 @default.
• W2128009076 creator A5078798958 @default.
• W2128009076 date "2015-04-15" @default.
• W2128009076 modified "2023-10-17" @default.
• W2128009076 title "Cell Surface Nucleolin Facilitates Enterovirus 71 Binding and Infection" @default.
• W2128009076 cites W1497883298 @default.
• W2128009076 cites W1517556208 @default.
• W2128009076 cites W1533516721 @default.
• W2128009076 cites W1536030981 @default.
• W2128009076 cites W1925850732 @default.
• W2128009076 cites W1936364165 @default.
• W2128009076 cites W1964374817 @default.
• W2128009076 cites W1965004469 @default.
• W2128009076 cites W1967780926 @default.
• W2128009076 cites W1971547467 @default.
• W2128009076 cites W1972881749 @default.
• W2128009076 cites W1975544839 @default.
• W2128009076 cites W1976420412 @default.
• W2128009076 cites W1978042026 @default.
• W2128009076 cites W1980609283 @default.
• W2128009076 cites W1980758890 @default.
• W2128009076 cites W1982516347 @default.
• W2128009076 cites W1988204994 @default.
• W2128009076 cites W1990439433 @default.
• W2128009076 cites W1991823190 @default.
• W2128009076 cites W1993400795 @default.
• W2128009076 cites W1994364053 @default.
• W2128009076 cites W1994466698 @default.
• W2128009076 cites W1995625848 @default.
• W2128009076 cites W2003542279 @default.
• W2128009076 cites W2004474576 @default.
• W2128009076 cites W2009356080 @default.
• W2128009076 cites W2012994594 @default.
• W2128009076 cites W2013566223 @default.
• W2128009076 cites W2016624762 @default.
• W2128009076 cites W2017402027 @default.
• W2128009076 cites W2024062560 @default.
• W2128009076 cites W2030493864 @default.
• W2128009076 cites W2032679727 @default.
• W2128009076 cites W2035255507 @default.
• W2128009076 cites W2035883892 @default.
• W2128009076 cites W2038314679 @default.
• W2128009076 cites W2039483976 @default.
• W2128009076 cites W2044502794 @default.
• W2128009076 cites W2047809394 @default.
• W2128009076 cites W2049807989 @default.
• W2128009076 cites W2050063411 @default.
• W2128009076 cites W2053347879 @default.
• W2128009076 cites W2071765876 @default.
• W2128009076 cites W2074257358 @default.
• W2128009076 cites W2082800992 @default.
• W2128009076 cites W2083705247 @default.
• W2128009076 cites W2085169891 @default.
• W2128009076 cites W2086443735 @default.
• W2128009076 cites W2087100112 @default.
• W2128009076 cites W2087293936 @default.
• W2128009076 cites W2099288944 @default.
• W2128009076 cites W2099315132 @default.
• W2128009076 cites W2100008113 @default.
• W2128009076 cites W2105078721 @default.
• W2128009076 cites W2107053547 @default.
• W2128009076 cites W2110005473 @default.
• W2128009076 cites W2126266785 @default.
• W2128009076 cites W2127383168 @default.
• W2128009076 cites W2134403134 @default.
• W2128009076 cites W2137471638 @default.
• W2128009076 cites W2140427533 @default.
• W2128009076 cites W2141292446 @default.
• W2128009076 cites W2146059615 @default.
• W2128009076 cites W2155657787 @default.
• W2128009076 cites W2158040058 @default.
• W2128009076 cites W2158547927 @default.
• W2128009076 cites W2160298781 @default.
• W2128009076 cites W2166648201 @default.
• W2128009076 cites W2169120688 @default.
• W2128009076 cites W85890770 @default.
• W2128009076 doi "https://doi.org/10.1128/jvi.03498-14" @default.
• W2128009076 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4442404" @default.
• W2128009076 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25673703" @default.
• W2128009076 hasPublicationYear "2015" @default.
• W2128009076 type Work @default.
• W2128009076 sameAs 2128009076 @default.
• W2128009076 citedByCount "82" @default.
• W2128009076 countsByYear W21280090762015 @default.
• W2128009076 countsByYear W21280090762016 @default.
• W2128009076 countsByYear W21280090762017 @default.
• W2128009076 countsByYear W21280090762018 @default.
• W2128009076 countsByYear W21280090762019 @default.

• next