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• W2128049245 abstract "Respiratory syncytial (RS) virus infects the epithelium of the respiratory tract. We examined the replication and maturation of RS virus in two polarized epithelial cell lines, Vero C1008 and MDCK. Electron microscopy of RS virus-infected Vero C1008 cells revealed the presence of pleomorphic viral particles budding exclusively from the apical surface, often in clusters. The predominant type of particle was filamentous, 80 to 100 nm in diameter, and 4 to 8 microns in length, and evidence from filtration studies indicated that the filamentous particles were infectious. Cytopathology produced by RS virus infection of polarized Vero C1008 cells was minimal, and syncytia were not observed, consistent with the maintenance of tight junctions and the exclusively apical maturation of the virus. Infectivity assays with MDCK cells confirmed that in this cell line, RS virus was released into the apical medium but not into the basolateral medium. In addition, the majority of the RS virus transmembrane fusion glycoprotein on the cell surface was localized to the apical surface of the Vero C1008 cells. Taken together, these results demonstrate that RS virus matures at the apical surface of polarized epithelial cell lines." @default.
• W2128049245 created "2016-06-24" @default.
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• W2128049245 creator A5039211320 @default.
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• W2128049245 date "1995-04-01" @default.
• W2128049245 modified "2023-10-16" @default.
• W2128049245 title "Respiratory syncytial virus matures at the apical surfaces of polarized epithelial cells" @default.
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• W2128049245 doi "https://doi.org/10.1128/jvi.69.4.2667-2673.1995" @default.
• W2128049245 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/188952" @default.
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