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- W2128049336 abstract "ABSTRACT Grb14 is a member of the Grb7 family of adapters and acts as a negative regulator of insulin-mediated signaling. Here we found that the protein kinase Cζ (PKCζ) interacting protein, ZIP, interacted with Grb14. Coimmunoprecipitation experiments demonstrated that ZIP bound to both Grb14 and PKCζ, thereby acting as a link in the assembly of a PKCζ-ZIP-Grb14 heterotrimeric complex. Mapping studies indicated that ZIP interacted through its ZZ zinc finger domain with the phosphorylated insulin receptor interacting region (PIR) of Grb14. PKCζ phosphorylated Grb14 under in vitro conditions and in CHO-IR cells as demonstrated by in vivo labeling experiments. Furthermore, Grb14 phosphorylation was increased under insulin stimulation, suggesting that the PKCζ-ZIP-Grb14 complex is involved in insulin signaling. The PIR of Grb14, which also interacts with the catalytic domain of the insulin receptor (IR) and inhibits its activity, was preferentially phosphorylated by PKCζ. Interestingly, the phosphorylation of Grb14 by PKCζ increased its inhibitory effect on IR tyrosine kinase activity in vitro. The role of ZIP and Grb14 in insulin signaling was further investigated in vivo in Xenopus laevis oocytes. In this model, ZIP potentiated the inhibitory action of Grb14 on insulin-induced oocyte maturation. Importantly, this effect required the recruitment of PKCζ and the phosphorylation of Grb14, providing in vivo evidences for a regulation of Grb14-inhibitory action by ZIP and PKCζ. Together, these results suggest that Grb14, ZIP, and PKCζ participate in a new feedback pathway of insulin signaling." @default.
- W2128049336 created "2016-06-24" @default.
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- W2128049336 date "2002-10-01" @default.
- W2128049336 modified "2023-10-18" @default.
- W2128049336 title "The Adapter Protein ZIP Binds Grb14 and Regulates Its Inhibitory Action on Insulin Signaling by Recruiting Protein Kinase Cζ" @default.
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- W2128049336 cites W1769997374 @default.
- W2128049336 cites W1967235396 @default.
- W2128049336 cites W1967776802 @default.
- W2128049336 cites W1968322499 @default.
- W2128049336 cites W1971498717 @default.
- W2128049336 cites W1973556619 @default.
- W2128049336 cites W1973724537 @default.
- W2128049336 cites W1973945575 @default.
- W2128049336 cites W1974075629 @default.
- W2128049336 cites W1976578056 @default.
- W2128049336 cites W1977795081 @default.
- W2128049336 cites W1981023296 @default.
- W2128049336 cites W1982573599 @default.
- W2128049336 cites W1983024391 @default.
- W2128049336 cites W1983374628 @default.
- W2128049336 cites W1993995347 @default.
- W2128049336 cites W2001717986 @default.
- W2128049336 cites W2004844986 @default.
- W2128049336 cites W2012678244 @default.
- W2128049336 cites W2013549179 @default.
- W2128049336 cites W2020346602 @default.
- W2128049336 cites W2024153969 @default.
- W2128049336 cites W2025781389 @default.
- W2128049336 cites W2030848654 @default.
- W2128049336 cites W2031082821 @default.
- W2128049336 cites W2031879122 @default.
- W2128049336 cites W2035230751 @default.
- W2128049336 cites W2046013104 @default.
- W2128049336 cites W2046124930 @default.
- W2128049336 cites W2046462167 @default.
- W2128049336 cites W2051294804 @default.
- W2128049336 cites W2051973322 @default.
- W2128049336 cites W2057036002 @default.
- W2128049336 cites W2059571622 @default.
- W2128049336 cites W2061965961 @default.
- W2128049336 cites W2065290052 @default.
- W2128049336 cites W2067471234 @default.
- W2128049336 cites W2069794424 @default.
- W2128049336 cites W2073913152 @default.
- W2128049336 cites W2077397396 @default.
- W2128049336 cites W2077971702 @default.
- W2128049336 cites W2078427707 @default.
- W2128049336 cites W2080115585 @default.
- W2128049336 cites W2082067420 @default.
- W2128049336 cites W2084817678 @default.
- W2128049336 cites W2085021339 @default.
- W2128049336 cites W2089519022 @default.
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- W2128049336 cites W2126203507 @default.
- W2128049336 cites W2141755885 @default.
- W2128049336 cites W2155288742 @default.
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- W2128049336 doi "https://doi.org/10.1128/mcb.22.20.6959-6970.2002" @default.
- W2128049336 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/139806" @default.
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