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• W2128083417 abstract "Peripheral blood cytopenias present a major problem in the management of patients with HIV infection. Their pathophysiology is likely to be multifactorial. although there is controversy as to whether haemopoietic progenitors are a target for HIV. In order to investigate the haemopoietic defect in HIV infection, we looked at bone marrow culture characteristics of marrow from eight HIV+ patients compared to normal controls. We performed long-term liquid culture (LTC) and colony forming assays for granulocytemacrophage (CFU-GM) and granulocyte. erythroid. megakaryocyte. macrophage (CFU-GEMM). In LTC we found normal stromal appearance and haemopoietic focus formation. There was no difference in colony assays of CFU-GM and CFU-GEMM between HIV+ and normal controls. Colonies taken from CFU-GM and CFU-GEMM were analysed for HIV DNA sequences, and we were able to detect HIV DNA in colonies from all HIV+ patients. Our results indicate that despite infection of haemopoietic progenitor cells by HIV, bone marrow function is preserved. This suggests that HIV-related cytopenias may be due to alternative mechanisms not present in our in vitro system." @default.
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• W2128083417 date "1992-12-01" @default.
• W2128083417 modified "2023-10-14" @default.
• W2128083417 title "Detection of HIV in haemopoietic progenitors" @default.
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• W2128083417 doi "https://doi.org/10.1111/j.1365-2141.1992.tb06956.x" @default.
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