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- W2128085280 abstract "A radioimmunoassay (RIA) method for alpha 1-fetoprotein (AFP) in the serum of rats is reported. The method is based on the preparation of purified and radiolabeled AFP, monospecific anti-AFP antiserum, and a modification of the coprecipitation-inhibition technique in 50% saturated ammonium sulfate. A combination of immunochemical and physiocochemical methods are used in the purification of the AFP. The purified AFP is virtually identical to the native, circulating AFP by immunological criteria and contains, at most, trace contamination with other materials. The RIA has a sensitivity that allows 25 ng AFP/ml of serum to be detected with reproducibility. This assay requires 20 mcl of serum and can be completed within a few hours. It is concluded that this RIA, dependent upon the preparation of monospecific anti-AFP, radioiodination of highly purified rat AFP and a modified Farr procedure, is a sensitive and reproducible RIA." @default.
- W2128085280 created "2016-06-24" @default.
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- W2128085280 date "1972-12-01" @default.
- W2128085280 modified "2023-09-23" @default.
- W2128085280 title "Radioimmunoassay for 1 -fetoprotein in the serum of rats." @default.
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