FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2128232666> ?p ?o ?g. }

• W2128232666 endingPage "4355" @default.
• W2128232666 startingPage "4343" @default.
• W2128232666 abstract "Cerebral cavernous malformations (CCM) are sporadic or inherited vascular lesions of the central nervous system characterized by dilated, thin-walled, leaky vessels. Linkage studies have mapped autosomal dominant mutations to three loci: ccm1 (KRIT1), ccm2 (OSM), and ccm3 (PDCD10). All three proteins appear to be scaffolds or adaptor proteins, as no enzymatic function can be attributed to them. Our previous results demonstrated that OSM is a scaffold for the assembly of the GTPase Rac and the MAPK kinase kinase MEKK3, for the hyperosmotic stress-dependent activation of p38 MAPK. Herein, we show that the three CCM proteins are members of a larger signaling complex. To define this complex, epitope-tagged wild type OSM or OSM harboring the mutation of F217-->A, which renders the OSM phosphotyrosine binding (PTB) domain unable to bind KRIT1, were stably introduced into RAW264.7 mouse macrophages. FLAG-OSM or FLAG-OSMF217A and the associated complex members were purified by immunoprecipitation using anti-FLAG antibody. OSM binding partners were identified by gel-based methods combined with electrospray ionization-MS or by multidimensional protein identification technology (MudPIT). Previously identified proteins that associate with OSM including KRIT1, MEKK3, Rac, and the KRIT1-binding protein ICAP-1 were found in the immunoprecipitates. In addition, we show for the first time that PDCD10 binds to OSM and is found in cellular CCM complexes. Other prominent proteins that bound the CCM complex include EF1A1, RIN2, and tubulin, with each interaction disrupted with the OSMF217A mutant protein. We further show that PDCD10 binds phosphatidylinositol di- and triphosphates and OSM binds phosphatidylinositol monophosphates. The findings define the targeting of the CCM complex to membranes and to proteins regulating trafficking and the cytoskeleton." @default.
• W2128232666 created "2016-06-24" @default.
• W2128232666 creator A5015664157 @default.
• W2128232666 creator A5023749711 @default.
• W2128232666 creator A5043226629 @default.
• W2128232666 creator A5047612906 @default.
• W2128232666 creator A5059164947 @default.
• W2128232666 creator A5060313679 @default.
• W2128232666 creator A5068904849 @default.
• W2128232666 date "2007-09-27" @default.
• W2128232666 modified "2023-10-13" @default.
• W2128232666 title "Proteomic Identification of the Cerebral Cavernous Malformation Signaling Complex" @default.
• W2128232666 cites W1560901632 @default.
• W2128232666 cites W1597678601 @default.
• W2128232666 cites W1965098419 @default.
• W2128232666 cites W1974616763 @default.
• W2128232666 cites W1989286785 @default.
• W2128232666 cites W1991872394 @default.
• W2128232666 cites W1993928278 @default.
• W2128232666 cites W1999032458 @default.
• W2128232666 cites W2005252392 @default.
• W2128232666 cites W2006986579 @default.
• W2128232666 cites W2009268510 @default.
• W2128232666 cites W2011874398 @default.
• W2128232666 cites W2013410321 @default.
• W2128232666 cites W2027052842 @default.
• W2128232666 cites W2031615571 @default.
• W2128232666 cites W2035293726 @default.
• W2128232666 cites W2043020898 @default.
• W2128232666 cites W2045153659 @default.
• W2128232666 cites W2046006262 @default.
• W2128232666 cites W2047433704 @default.
• W2128232666 cites W2051691391 @default.
• W2128232666 cites W2052385316 @default.
• W2128232666 cites W2052394197 @default.
• W2128232666 cites W2056005326 @default.
• W2128232666 cites W2058028915 @default.
• W2128232666 cites W2059572899 @default.
• W2128232666 cites W2065155413 @default.
• W2128232666 cites W2068719237 @default.
• W2128232666 cites W2072000480 @default.
• W2128232666 cites W2075108450 @default.
• W2128232666 cites W2088454405 @default.
• W2128232666 cites W2099043491 @default.
• W2128232666 cites W2110537012 @default.
• W2128232666 cites W2112958616 @default.
• W2128232666 cites W2117713381 @default.
• W2128232666 cites W2121850322 @default.
• W2128232666 cites W2132811240 @default.
• W2128232666 cites W2165023373 @default.
• W2128232666 cites W2167212627 @default.
• W2128232666 cites W2255636874 @default.
• W2128232666 cites W2273663913 @default.
• W2128232666 cites W2402594376 @default.
• W2128232666 doi "https://doi.org/10.1021/pr0704276" @default.
• W2128232666 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17900104" @default.
• W2128232666 hasPublicationYear "2007" @default.
• W2128232666 type Work @default.
• W2128232666 sameAs 2128232666 @default.
• W2128232666 citedByCount "127" @default.
• W2128232666 countsByYear W21282326662012 @default.
• W2128232666 countsByYear W21282326662013 @default.
• W2128232666 countsByYear W21282326662014 @default.
• W2128232666 countsByYear W21282326662015 @default.
• W2128232666 countsByYear W21282326662016 @default.
• W2128232666 countsByYear W21282326662017 @default.
• W2128232666 countsByYear W21282326662018 @default.
• W2128232666 countsByYear W21282326662019 @default.
• W2128232666 countsByYear W21282326662020 @default.
• W2128232666 countsByYear W21282326662021 @default.
• W2128232666 countsByYear W21282326662022 @default.
• W2128232666 countsByYear W21282326662023 @default.
• W2128232666 crossrefType "journal-article" @default.
• W2128232666 hasAuthorship W2128232666A5015664157 @default.
• W2128232666 hasAuthorship W2128232666A5023749711 @default.
• W2128232666 hasAuthorship W2128232666A5043226629 @default.
• W2128232666 hasAuthorship W2128232666A5047612906 @default.
• W2128232666 hasAuthorship W2128232666A5059164947 @default.
• W2128232666 hasAuthorship W2128232666A5060313679 @default.
• W2128232666 hasAuthorship W2128232666A5068904849 @default.
• W2128232666 hasConcept C104317684 @default.
• W2128232666 hasConcept C104397665 @default.
• W2128232666 hasConcept C143065580 @default.
• W2128232666 hasConcept C153911025 @default.
• W2128232666 hasConcept C170493617 @default.
• W2128232666 hasConcept C174510640 @default.
• W2128232666 hasConcept C183786373 @default.
• W2128232666 hasConcept C185592680 @default.
• W2128232666 hasConcept C53645450 @default.
• W2128232666 hasConcept C55493867 @default.
• W2128232666 hasConcept C62478195 @default.
• W2128232666 hasConcept C69125201 @default.
• W2128232666 hasConcept C71829478 @default.
• W2128232666 hasConcept C86803240 @default.
• W2128232666 hasConcept C95444343 @default.
• W2128232666 hasConceptScore W2128232666C104317684 @default.
• W2128232666 hasConceptScore W2128232666C104397665 @default.
• W2128232666 hasConceptScore W2128232666C143065580 @default.

• next